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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    In-silico characterization and comparative study of iron and zinc regulatory gene families NAS, YSL and ZIP in cereal crops
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-02) Bhattacharya, Tithi; Sundip Kumar
    Micronutrient deficiency, especially iron and zinc deficiency in diet is a major reason of malnutrition in developing countries. Most of the population in developing countries is dependent on their staple crops as a source for their nutrition. Hence, developing biofortified crop varieties can be a promising way to overcome malnutrition of these minerals. Lack of genetic variation in major staples like rice and wheat is a major drawback in biofortification by conventional breeding method. Bioinformatics tools can be used as an alternative in biofortification of food grains. Iron homeostasis is a process by which the availability of iron is properly maintained in the plant body. In order to regulate this process a number of genes play a key role. Among these nicotinamine synthase (NAS), yellow stripe like (YSL) protein and zinc transporter protein (ZIP) are the most important and largely reported genes in cereal crops for iron and zinc uptake, transport and its storage. The comparative study of these iron homeostasis genes in related species helps to describe the evolutionary relationship, their nucleotide and amino acid profiling along with motif regions. During the present investigation in silico identification, characterization and comparative study of three iron homeostasis gene families viz. NAS, YSL and ZIP is conducted in five major cereal crops namely rice, wheat, maize, barley and barn yard millet. Various physico-chemical parameters such as molecular weight, isoelectric point, and aliphatic index are computed. Nicotinamine synthase gene (NAS), yellow- stripe like (YSL) protein and ZIP (ZRT-IRT) like proteins are involved in metal transport in plants. The respective sequences are retrieved from NCBI database in fasta format and different analysis are carried out. Phylogenetic tree is constructed using neighbour joining method for all the three groups together (NAS, YSL and ZIP genes) and individually for the three groups and the results are analyzed. Multiple sequence alignment study showed that amino acids namely glycine and phenylalanine are evolutionary conserved among these gene families. Phylogenetic analysis revealed that NAS and YSL gene family divided into two clusters A and B. On the other hand, the genes belonging to ZIP family divided into three clusters namely A, B and C. Conserved domain search explained that AdoMet_MTases superfamily with seven domain hits found in NAS gene family, YSL gene family contain OPT superfamily having four domain hits and ZIP gene family having five domain hits of ZIP superfamily. Physicochemical analysis is done using PROTPARAM tool. Target P-1.1 is used to determine the sub – cellular location of proteins. Some of the proteins are signal peptide, few are mitochondrial transfer peptide, while the analysis of most of the proteins show that they belong to some other location other than signal peptide, mitochondrial transfer peptide, chloroplast transfer peptide and thylakoid luminal transfer peptide. Secondary structure is predicted using Chou- Fasman secondary structure prediction software. Comparing the average percentage of secondary structure it was found that, alpha helix is the most predominant conformation revealing the higher level of conservation and stability of protein structure. Highest percentage of alpha helix was detected in ZIP family and beta strand found in YSL whereas highest percentage of turn was found in NAS gene family. The information generated out of this comparative study of iron homeostasis regulatory genes in cereal crops may be helpful for better understanding of iron homeostasis in cereals which may subsequently be utilized for iron and zinc biofortification in cereal crops.