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Govind Ballabh Pant University of Agriculture and Technology, Pantnagar

After independence, development of the rural sector was considered the primary concern of the Government of India. In 1949, with the appointment of the Radhakrishnan University Education Commission, imparting of agricultural education through the setting up of rural universities became the focal point. Later, in 1954 an Indo-American team led by Dr. K.R. Damle, the Vice-President of ICAR, was constituted that arrived at the idea of establishing a Rural University on the land-grant pattern of USA. As a consequence a contract between the Government of India, the Technical Cooperation Mission and some land-grant universities of USA, was signed to promote agricultural education in the country. The US universities included the universities of Tennessee, the Ohio State University, the Kansas State University, The University of Illinois, the Pennsylvania State University and the University of Missouri. The task of assisting Uttar Pradesh in establishing an agricultural university was assigned to the University of Illinois which signed a contract in 1959 to establish an agricultural University in the State. Dean, H.W. Hannah, of the University of Illinois prepared a blueprint for a Rural University to be set up at the Tarai State Farm in the district Nainital, UP. In the initial stage the University of Illinois also offered the services of its scientists and teachers. Thus, in 1960, the first agricultural university of India, UP Agricultural University, came into being by an Act of legislation, UP Act XI-V of 1958. The Act was later amended under UP Universities Re-enactment and Amendment Act 1972 and the University was rechristened as Govind Ballabh Pant University of Agriculture and Technology keeping in view the contributions of Pt. Govind Ballabh Pant, the then Chief Minister of UP. The University was dedicated to the Nation by the first Prime Minister of India Pt Jawaharlal Nehru on 17 November 1960. The G.B. Pant University is a symbol of successful partnership between India and the United States. The establishment of this university brought about a revolution in agricultural education, research and extension. It paved the way for setting up of 31 other agricultural universities in the country.

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  • ThesisItemOpen Access
    Isolation, cloning and in silico analysis of drought responsive EcMyb1 transcription factor gene from Eleusine coracana
    (G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand), 2021-01) Bhatt, Megha; Lohani, Pushpa
    Myb genes are members of a family of transcription factors that play an important role in cell division, differentiation, response to abiotic stress etc. It has been reported that MYB transcription factor play an important role as a master regulator and modulates the expression of various downstream genes involved in drought stress response. The partial sequence of Ecmyb1 gene was isolated and submitted to NCBI with accession number JN107890.1. In present study, full length Ecmyb1 gene was isolated and cloned. The cloned fragment was sequenced and submitted to NCBI with accession number MT312253. For expression analysis of Ecmyb1 gene, finger millet drought tolerant variety PRM 6107 was given three different abiotic stress treatment i.e. drought, salt and heat stress. The expression of the gene increased from 12 to 28 fold under drought stress as compared to control. Similarly, in salt and heat treated plants, expression increased from 7 to 29 fold and 19 to 46 fold respectively. The cloned gene was introgressed into Arabidopsis thaliana plant by floral dip method. The transformed plants were selected by hygromysin screening and confirmed by PCR using hygromycin primers. Insilco analysis of Ecmyb1 gene was carried out using various softwares. Promoter analysis of Ecmyb1 gene revealed the presence of various regulatory elements like ABA responsive elements (CACT GG), stress responsive elements (AAGG GG), Myb recognition sites (CAACAG), Myb binding sites (CAAC TG), CpG island and tandem repeats. Homology and phylogenetic analysis of Ecmyb1 gene and amino acid sequences showed similarity with other MYB proteins such as Sateria italica, Hordeum vulgare, Saccharum barberi and Oryza sativa etc. Motif analysis predicted the presence of HTH domain in EcMYB1 protein. Subsequently, it was computed that EcMYB1 protein is water soluble, thermostable and slightly acidic in nature. Additionally, it was observed that EcMYB1 protein contains 28% alpha helix, 5.43% extended strand, 2.57% beta turn and 64% random coil. 3-D structure of EcMYB1 using swiss model predicted that 90% aminoacid residues were in Ramachandran favored region with no bad angles. It indicated that EcMYB1 protein may exist in nature.