Loading...
Kerala Veterinary and Animal Sciences University, Wayanad
Browse
425 results
Search Results
ThesisItem Open Access LACTATION PERFORMANCE AND MILK COMPOSITION OF ATTAPPADY BLACK GOATS(COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2017-08-30) SUDHARSAN M.; A. KannanThesisItem Open Access COMPARISON OF DIFFERENT MILK REPLACERS ON GROWTH PERFORMANCE OF MALABARI KIDS(COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2018-12-30) N. SASIKALA; Sabin GeorgeAn experiment was conducted to study the effect of feeding different milk replacers in Malabari kids on their growth performance for the period of two months. Twenty four healthy Malabari kids of four days of age were selected and divided into four groups of six each, as uniformly as possible with regard to age and body weight and were allotted randomly to different experimental rations. Experimental rations were T1 (control, dam’s milk), T2 (Milk replacer based on whey powder), T3 (Milk replacer based on groundnut oilcake) and T4 (Milk replacer based on coconut oilcake). Dam’s milk and milk replacers were fed to kids at the rate of 1/6th and 1/8th of the body weight during first month and second month respectively. Kid starter and fresh green grass were offered to the kids from 15th day onwards to all the experimental kids throughout the experimental period. Data on body weight gain, body measurements, daily dry matter intake, feed conversion efficiency, haematological and serum biochemical parameters such as haemoglobin, plasma total protein, serum glucose, urea nitrogen, serum calcium and phosphorus, faecal consistency score, incidence of diseases and techno economics of production were employed for evaluation of different treatments. Average daily gain and total weight gain were 63 g and 3.98 kg, 62 g and 3.91 kg, 49 g and 3.07 kg and 47 g and 2.96 kg, respectively for kids fed T1, T2, T3 and T4. Average daily gain and total weight gain were significantly higher in T1 and T2 compared to the kid fed T3 and T4. Over all mean of body length at two months ranged from 37.75 to 39.92 cm. Mean heart girth ranged from 35.50 to 36.33 cm. Mean height at withers ranged from 36.83 to 39.92 cm. Body measurements showed no significant difference between the treatments. Total dry matter intake of kids was 6.93, 5.23, 4.80 and 4.88 kg, respectively for T1, T2, T3 and T4. Total dry matter intake was significantly higher in T1 than T2, T3 and T4. Feed conversion efficiency for kids fed T1, T2, T3 and T4 was 1.73, 1.34, 1.56 and 1.65, respectively. The haematological and serum biochemical parameters such as haemoglobin, plasma total protein, serum calcium, phosphorus, glucose, blood urea nitrogen did not show any significant difference between the treatments and the values were within the normal range reported for the species. Faecal consistency score was comparatively higher in the first fortnight than the following fortnights and the values were similar between the treatments. Out of the total cases of diarrhea, incidence was more in kids fed T4 (30 per cent). Respiratory illness was noticed higher in kids fed T3 and T4 than T1 and T2. Majority of the cases of bloat was noticed in T4 group. The feed cost per kg body weight gain for the kids fed T1, T2, T3 and T4 were Rs. 580.02, 139.60, 176.94, 179.78, respectively. Feed cost / kg gain was significantly lower in kids fed with T2 followed by T3, T4 and T1 groups. From the results obtained on the present study it could be inferred that kids in T2 had similar growth performance as that of control (T1) group. Feed cost per kg gain in T2, T3 and T4 was better than T1. Therefore it could be concluded that milk replacer based on whey powder can be given to the kids as an alternative to milk without compromising the growth performance.ThesisItem Open Access HEPATOPROTECTIVE EFFECT OF ECLIPTA PROSTRATA (L.) L. LEAVES ON EXPERIMENTALLY INDUCED AFLATOXICOSIS IN BROILER CHICKEN(COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, 2018-11-30) PRIYA. K; Preethy JohnThe study was aimed to investigate the hepatoprotective effect of E. prostrata (Kayyonni) leaf powder on experimentally induced aflatoxicosis in broiler chicken. The leaf powder was subjected to preliminary phytochemical screening to find out the active principles present in it. Aflatoxin was produced in maize using the culture Aspergillus flavus NRRL 6513. The maize culture powder yielded 143.48 ppm of aflatoxin. This mouldy maize was incorporated in experimental feed to arrive 500 ppb of aflatoxin. Sixty Cobb400 day old broiler chicks weighing 50 ± 5 g were randomly divided into six groups comprising 10 birds in each group. The birds were maintained under deep litter system and provided with ad libitum water and feed throughout the experimental period. All the birds were vaccinated as per the standard schedule. Aflatoxicosis was experimentally induced in all groups except T1 and T3 by giving 500 ppb of aflatoxin B1 (AFB1) from eighth day of age onwards. The group T1 was kept as normal control and T2 as toxic control. T3 was fed with E. prostata leaf powder at 0.2 per cent level. The leaf powder of E. prostrata was given to T4, T5 and T6 at dose rates of 0.05, 0.1 and 0.2 per cent respectivelyBody weight was recorded at weekly intervals and the blood was collected from the wing vein on days 7, 21 and 42. Serum was separated and used for the estimation of biochemical parameters such as aspartate transaminase (AST), creatine kinase (CK), cholesterol and total proteins. On the day 42, all the birds were sacrificed; detailed post- mortem examination was conducted. Liver samples were taken to estimate antioxidant parameters such as lipid peroxidation (LPO) and reduced glutathione (GSH). Representative liver samples were also taken and preserved with 10 per cent neutral buffered formalin for histopathological examinationThe preliminary phytochemical screening of E. prostrata leaf powder revealed the presence of steroid, tannins, flavonoids, diterpenes, tripterpenes and saponin.Treatment with E. prostrata leaves powder revealed hepatoprotection in dose dependent manner which is indicated by significant (P<0.05) reduction in the level of serum AST and increase in the level of cholesterol and total protein. The oxidative stress induced by aflatoxin in liver was reduced to a great extend as indicated by the increased level of reduced glutathione and decrease in the lipid peroxidation. Histopathological examination of liver showed regenerative changes in a dose dependent manner when compared with that of normal control group. Thus, it could be concluded that E .prostrata leaf powder had marked antioxidant and hepatoprotective effect on experimentally induced aflatoxicosis in broiler chickenThesisItem Open Access Development of protocol for management of hoof problems in crossbred cattle(COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2018-09-03) POOJA U; Anil K.SThe present study was conducted to identify predisposing factors of hoof problems in crossbred dairy cattle and to develop a protocol for the management of hoof problems in crossbred cattle of Kerala. A total of 45 private farms in Thrissur and Palakkad districts and 2 farms under KVASU were taken for the study. The study covered small scale farms(1-2 animal), medium scale farms(3-10 animals) and large scale farms(>10animals) of both districts and two organized farms viz, University livestock farm, Mannuthy, and Cattle breeding farm, Thumburmuzhy. Hoof problems were identified using In stall lameness indicators and severity was assessed using locomotion score and body condition score of the animal. The large scale farms had maximum incidence (41.2 per cent) followed by medium (26.7 per cent) and small scale farms (20.0 per cent). The incidence in organized farms was thirteen per cent. The incidence of hoof problem was more in large-medium land holdings (56.3 per cent) farms where as in marginal-small farms incidence was less (10.3 per cent). The incidence of hoof problem in crossbred HF and crossbred Jersey were 31.7 per cent and 16.7 per cent respectively. The effect of age and body weight of the animal was found to be significant (p0.05). The animal reared on concrete floor had more incidence of hoof problems (46.4 per cent) compared to stone pavement which was (20 per cent). In slippery/pitted floor hoof disorders for cattle was 36.8 per (p<0.05). The incidence of hoof problem was more with asbestos as roof material (59.1 per cen). As frequency of animal bathing increases the incidence of hoof problem was found more (46.7 per cent). The average lactation yield per day was less than five litres in all animals with hoof problem and also in early stage of lactation hoof problem was found more. The animal with parity two (61.5%) is found to have more incidence hoof disorders compared to one and three. The 75 percent of animal having hoof problems was also suffering from anestrum (p<0.05). The incidence of hoof problem was only 13.6 per cent in farms with provision of foot bath whereas it was 44 per cent in farms without footbath although. The occurrence of hoof problem was more in rainy season (70.2 per cent) than in summer and post monsoon. In 2.3 per cent of farms culling was done due to hoof problems. The incidence of hoof problem was 20.7 per cent in farms where hoof trimming was carried out and 44.4 per cent incidence in farms without hoof trimmi. The average locomotion score in private farms was 4.31±0.96 whereas in organized farms it was 3.97±0.133. The average body condition score was 1.82±0.099 for private farms and for organized farms was 2.38±0.140 (p<0.05) . From this study it can be concluded that hoof disorders are a serious problem in crossbred cattle under field conditions and all efforts are needed to control by providing adequate inputs.ThesisItem Open Access A COMPREHENSIVE APPROACH FOR DIAGNOSIS OF LEPTOSPIROSIS IN DOMESTIC PIGS(COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2018-09-30) P. S. RESHMA; M. MiniLeptospirosis is a worldwide zoonotic disease that in pigs primarily causes reproductive disturbances. Samples collected from Centre for Pig Production and Research (CPPR), Mannuthy and selected private farms in Thrissur were used for the present study. Serum samples (n=103) were tested using microscopic agglutination test (MAT) and an overall seropositivity of 35.92 per cent could be detected. The serogroup Pomona (45.95 per cent) was the most prevalent among the 37 positive samples followed by serogroups Grippotyphosa (24.32 per cent), Canicola (13.51 per cent), Icterohaemorrhagiae (10.81 per cent) and Tarassovi (5.41 per cent). Samples of serum (n=56), whole blood (n=52) and aborted foetus (n=7) when tested using PCR for the presence of lipl32 gene of Leptospira, DNA of three whole blood samples and one aborted foetus amplified the gene producing an expected 767 bp amplicon. Latex agglutination test (LAT) and indirect fluorescent antibody test (FAT) was standardised using seven-day-old reference cultures of Leptospira interrogans serovar Pomona as positive controls at 10-fold dilutions. However, none of the abortion samples tested using LAT and FAT were positive. Attempts for isolation from positive samples were also made, but Leptospira could not be isolated from any of the foetal membrane, liver, kidney samples from the PCR positive animals. Hence in the present study, a seroprevalence of 35.92 per cent for leptospirosis among pigs in Thrissur district was detected using MAT, and PCR was found to be the most sensitive method for directly detecting the presence of the organism in clinical samples, compared to LAT, FAT and culture.ThesisItem Open Access VIRULENCE GENE PROFILING OF Escherichia coli FROM BOVINE MASTITIS AND THERAPEUTIC MANAGEMENT OF COLIFORM MASTITIS(Kerala Veterinary and Animal Sciences Mannuthy, Thrissur, 2019-12-30) M.REVATHI; S. SulficarColiforms are the major etiological agents of bovine mastitis which is an economically devastating disease causing substantial loss to the dairy farmers through reduction in the milk production. The present study was conducted to detect the virulence genes of E. coli by Polymerase Chain Reaction (PCR), to study the therapeutic efficacy of various antibiotics in E. coli mastitis and to identify the risk factors associated with coliform mastitis. Epidemiological investigations revealed that affected cows were in younger age and early stage of lactation having average milk yield. Absence of hygienic practices for clean milk production such as teat dipping resulted in poor udder hygiene which led to the occurrence of coliform mastitis. The affected animals had elevated temperature with varying degree of udder oedema and pale yellow coloured milk. In the present study, out of 168 animals affected with clinical mastitis, 123 bacterial isolates could be isolated of which 26 constituted coliforms such as Escherichia coli (14), Klebsiella spp. (10), Enterobacter spp. (1) and Citrobacter spp. (1) with the prevalence of 21.13 per cent and the remaining were Gram positive isolates. In vitro antibacterial sensitivity test of coliform isolates revealed that ceftizoxime was the most sensitive drug. Treatment of affected cases was done with sensitive antibiotics according to the antibiogram, fluid therapy, flunixin meglumine and trisodium citrate were administered based on the resolution of clinical signs. After treatment, clinical recovery with increase in milk yield was noticed in all the cases but for the resolution of udder oedema. Haematological analysis prior and after treatment revealed significant increase in total blood count and reduction in leukocyte counts. Polymerase chain reaction was performed to identify the virulence genes of E.coli viz., traT, stx 1, stx 2, eaeA and aerobactin (iucD) which revealed that among 14 isolates, two were positive for traT gene, two were positive for aerobactin gene and one was positive for stx 2 gene. Thus, the present study revealed the presence of virulence genes among E.coli isolates causing bovine mastitis.ThesisItem Open Access VIRULENCE GENE PROFILING OF Escherichia coli FROM BOVINE MASTITIS AND THERAPEUTIC MANAGEMENT OF COLIFORM MASTITIS(Kerala Veterinary and Animal Sciences Mannuthy, Thrissur, 2019-09-30) M.REVATHI; S. SulficarColiforms are the major etiological agents of bovine mastitis which is an economically devastating disease causing substantial loss to the dairy farmers through reduction in the milk production. The present study was conducted to detect the virulence genes of E. coli by Polymerase Chain Reaction (PCR), to study the therapeutic efficacy of various antibiotics in E. coli mastitis and to identify the risk factors associated with coliform mastitis. Epidemiological investigations revealed that affected cows were in younger age and early stage of lactation having average milk yield. Absence of hygienic practices for clean milk production such as teat dipping resulted in poor udder hygiene which led to the occurrence of coliform mastitis. The affected animals had elevated temperature with varying degree of udder oedema and pale yellow coloured milk. In the present study, out of 168 animals affected with clinical mastitis, 123 bacterial isolates could be isolated of which 26 constituted coliforms such as Escherichia coli (14), Klebsiella spp. (10), Enterobacter spp. (1) and Citrobacter spp. (1) with the prevalence of 21.13 per cent and the remaining were Gram positive isolates. In vitro antibacterial sensitivity test of coliform isolates revealed that ceftizoxime was the most sensitive drug. Treatment of affected cases was done with sensitive antibiotics according to the antibiogram, fluid therapy, flunixin meglumine and trisodium citrate were administered based on the resolution of clinical signs. After treatment, clinical recovery with increase in milk yield was noticed in all the cases but for the resolution of udder oedema. Haematological analysis prior and after treatment revealed significant increase in total blood count and reduction in leukocyte counts. Polymerase chain reaction was performed to identify the virulence genes of E.coli viz., traT, stx 1, stx 2, eaeA and aerobactin (iucD) which revealed that among 14 isolates, two were positive for traT gene, two were positive for aerobactin gene and one was positive for stx 2 gene. Thus, the present study revealed the presence of virulence genes among E.coli isolates causing bovine mastitis.ThesisItem Open Access ASSESSMENT OF PULMONARY FUNCTION IN RESPIRATORY AND CARDIAC DISORDERS OF DOG(COLLEGE OF VETRINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2019-09-30) SWATHI S; S. AjithkumarEighteen adult Labrador retriever dogs of either sex above 1 year of age presented to the University Veterinary hospital, Mannuthy with history of clinical signs suggestive of cardio-pulmonary disease were considered for the study. Based on clinical, radiographic, electrocardiographic and echocardiographic examination, respiratory and cardiac diseases were confirmed in 18 dogs. The study consisted of four groups. Group A: Control (Apparently healthy animals) (n=6), Group B: Animals with upper respiratory tract diseases (n=6), Group C: Animals with lower respiratory tract diseases (n=6) and Group D: Animals with cardiac involvement showing respiratory signs (n=6). These animals were subjected to pulmonary function tests (spirometry and arterial blood gas analysis). The different types of upper respiratory tract diseases diagnosed in canine patients under study were tracheal stenosis (33.33 %), tracheitis (33.33 %), tracheal collapse (16.66 %) and pharyngitis (16.66 %). The diseases were more common in middle aged male dogs. The clinical signs included nasal discharge, respiratory distress, dyspnoea, cough, syncope, snoring and anorexia. Auscultation of trachea revealed inspiratory dyspnoea, stridor and stertor. The radiographic findings were evidence of narrowing of trachea in the cervical region and thoracic inlet, mild to moderate thickening of tracheal membrane and enlargement of pharyngeal region. In Group B, pneumonia, tracheobronchitis, pleural effusion and metastasis were noted in 50, 16.66, 16.66 and 16.66 per cent of the cases, respectively. The diseases were more common in middle aged male dogs. Respiratory abnormalities like dyspnoea, tachypnoea, cough (productive or non productive), fever, cyanosis and wheezing, crackles and harsh respiratory sounds on auscultation of lungs were the common clinical examination findings recorded. Radiographic appearance of lung pathologies in dogs with lower respiratory tract diseases showed bronchial, alveolar, vascular and interstitial or mixed patterns, peribronchial markings, increased soft tissue opacity, obscured cardiac silhouette and diaphragm, air bronchograms, lobar sign, lung consolidation and multiple miliary lesions depending upon the involvement of the anatomical structures. The cardiac disorders diagnosed were dilated cardiomyopathy (66.66 %), mitral valvular disease (16.66 %) and idiopathic pericardial effusion (16.66 %). The main clinical signs included anorexia, dyspnoea, cough, exercise intolerance, weak femoral pulse, ascites, limb oedema, syncope, tachycardia, murmurs, pulmonary crackles and muffled heart sounds on thoracic auscultation. Electrocardiographic and radiographic findings suggested of chamber enlargement and pericardial effusion was confirmed by echocardiography. The shape of the spirometric flow-volume loops obtained from control group was similar to the alphabet ‘D’ in appearance. Flattening and prolonged phase of inspiratory and expiratory portion of the loops were seen in dogs with upper respiratory tract and lower respiratory tract diseases, respectively. Flattening of both inspiratory and expiratory portion of the loops was observed in dogs with cardiac diseases. Significant decrease was noticed in the mean values of tidal volume in Group B, C and D in comparison to normal healthy animals. A significantly higher values of respiratory rate were recorded in group C and D in comparison to healthy animals and no difference was observed between Group A and B. There was significant decrease in the mean values of inspiratory time in Group C and D, whereas the mean values of inspiratory time in Group B was significantly increased compared to healthy animals. The mean values of expiratory to inspiratory time ratio in Group C and D showed significant increase, whereas mean values of expiratory to inspiratory time ratio in Group B showed significant decrease when compared to Group A. Arterial blood gas analysis revealed significant evidence of hypoxemia in affected dogs. Statistically significant decrease was noticed in the mean values of partial pressure of oxygen and saturated oxygen and significant increase was noticed in the mean values of Alveolar-arterial (A-a) gradient in Group B, C and D compared to apparently healthy animals.ThesisItem Open Access EVALUATION OF EFFLUENT TREATMENT METHODS IN DAIRY FARM(Kerala Veterinary and Animal Sciences Mannuthy, Thrissur, 2018-09-30) JONATHAN JAMES MOONNUMACKAL; A. KannanA study was conducted to evaluate the efficacy of different effluent treatment methods in dairy farm and to analyze cost effectiveness of effluent treatments. An initial survey was conducted among hundred farms of Thrissur district using a well structured questionnaire and data was collected regarding the farming system and their effluent management. The farms were categorised into small, medium and large. Six farms from each category were selected randomlyfor effluent collection in two different season namely summer and monsoon. Effluent was collected in the morning at 8am and evening at 5pm and pooled together. Representative sample (15 litres) of pooled effluent was taken from each farm and was subjected for different treatment protocols. After the end of each treatment water quality parameters were analysed. From the data of survey, it was found that there was a positive correlation between number of animals (96 per cent) and total water used (98 per cent) with effluent output. Effluent subjected with different treatment protocols (C- control, T1- Screening, T2- sedimentation, T3- Filtration and T4- chlorination). After the end of each treatment, the water were subjected to evaluation of quality parameters like colour, Total Dissolved Solids (TDS), pH, turbidity, Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Coliform count (TCC) and E. coli count. Data were compared with Indian Standards for reuse in dairy farm.The colour (Hazen) of water indicated that control (958.50 ± 4.18) was significantly higher than the rest of treatments. There was no significant difference between colour value of water from T3 (266.80 ± 5.8) and T4 (249.13 ± 8.66). T3 (filtered water) and T4 (chlorinated water) were lesser due to the respective treatments. The TDS (mg/L)of water from treatments T3 (1404.47 ± 31.68), T2 (2358.63 ± 34.52), T4 (2408.05 ±48.23) and T1 (3354.55 ± 29.65) with increasing trend were significantly lower than that of control (5161.22 ± 28.94). T2 (2358.63 ± 34.52) and T4 (2408.05 ± 48.23) were not significantly different from each other and T4 was significantly higher than that of T3. The pH of water from control (8.20 ± 0.07)and T1 (8.19 ± 0.05), T3 (7.40 ± 0.05)and T4 (7.26 ± 0.06)were significantly not different from each other where T2 (6.98 ± 0.10)was significantly different from all other treatments. The turbidity (NTU) of water from control (718.80 ± 6.86)and T1 (669.36 ± 10.16), T2 (76.38 ± 2.02)and T3 (42.26 ± 1.53) were significantly different from each other where T3 (42.26 ± 1.53) and T4 (38.00 ± 1.74)were not significantly different from each other. The BOD 5 (mg/L) of water from control (154.30 ± 1.31), T1 (131.75 ± 1.31), T2 (77.16 ± 1.31), T3 (52.41 ± 1.31) and T4 (41.44 ± 1.31) were significantly different from each other. The COD (mg/L) of water from control (1447.83 ± 15.04), T1 (1222.08 ± 19.40), T2 (672.00 ± 6.62), T3 (426.80 ± 10.37) and T4 (316.50 ± 8.51) were significantly different from each other. The TCC (MPN/100 ml) of water for control, T1, T2, T3 and T4 were 22.25 ± 0.27, 21.44 ± 0.27, 12.93 ± 0.27, 11.12 ± 0.27 and < 1.80 ± 0.27respectively.Control and T1 were having highest and T4 was having least TCC. The E. coli count (MPN/100 ml) of water for control, T1, T2, T3 and T4 were 22.22 ± 0.27, 21.13 ± 0.27, 12.93 ± 0.27, 11.12 ± 0.27and <1.80 ± 0.27respectively.Control and T1 were having highest and T4 was having least E. coli count.Effect of effluent treatments between the farm size revealed that parameters like pH, turbidity, COD and BOD 5 showed significant difference between treatment and farms and parameters like colour, TDS, TCC and E. coli had no significant difference between treatment and farms. The pH value of water from small farm (8.40 ± 0.12) was not significantly different from medium farm (8.32 ± 0.12)but significantly different from large farm (7.94 ± 0.12) in the control group. The turbidity (NTU) of water was significant different between treatments and farms. There was no significant difference between T3 and T4 in small, medium and large farms. The COD (mg/L) of water in small, medium and large farms, control (small- 1401.08 ± 26.05, medium- 1378.05 ± 26.05 and large- 1564.16 ± 26.05)were significantly higher (p<0.05) than that of T4, T3, T2 and T1. The BOD 5 (mg/L) was significant difference between treatments and farms. In small, medium and large farms, control was significantly higher (p<0.05) than that of T4, T3, T2 and T1.`Effect of effluent treatment between the seasons revealed that for all the water quality parameters like colour, TDS, pH, turbidity, COD, BOD5, TCC and E. coli count had no significant difference between treatment and seasons. The water quality parameters colour, TDS, pH, turbidity, Total Coliform Count and E. coli count were within the limits of Indian standards IS 10500. COD and BOD 5 were slightly higher than the limits of the Indian standards (IS 10500), so this water must be treated for reuse. The overall results in the study indicated that T4 having better efficacy compared to all other treatments. The results of the study indicated that the wastewater generated in the dairy farm can be effectively treated using the four stage treatment (C- control, T1- Screening, T2- sedimentation, T3- Filtration and T4- chlorination) in this study and the reclaimed water can be reused in the dairy farms for cleaning the shed, toconserve water and to reduce the water body contamination. The cost of the fourth treatment is comparatively higher however effectiveness of effluent treatment indicated that T4 is better in comparison with other treatments.
