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Kerala Veterinary and Animal Sciences University, Wayanad

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Author: Ambily, T R ×
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    ThesisItemOpen Access
    ASSESSMENT AND ALLEVIATION OF TRANSITION STRESS FROM PREGNANCY TO LACTATION IN CROSSBRED DAIRY CATTLE
    (College of Veterinary and animal Science,Mannuthy, 2017) Ambily, T R; Beena, V
    The study was conducted to assess the transition stress in 24 crossbred dairy cattle from 220 days of pregnancy to 30 days post-partum with supplementation of Se and/or vitamin E. The animals were randomly allocated to four groups (G0, G1, G2 and G3) with six animals in each group and were subjected to four dietary treatments as G0- adult cattle ration, G1- adult cattle ration + 0.3 ppm Se, G2 - adult cattle ration + vitamin E @ 1000 IU and G3- adult cattle ration + 0.3 ppm Se+ vitamin E @ 1000 IU. Supplementation started at 220 days of pregnancy and continued up to 30 days post-partum. Blood and serum samples were collected on 220, 250 days of pregnancy, day of calving and on 30 days post-partum. Data on physiological parameters, haematological parameters, biochemical parameters and environmental parameters were evaluated. The results obtained after statistical analysis for physiological parameters like birth weight of calves, 30 days milk yield and SCC in milk did not show significant difference between supplemented and control animals. Haematological parameters indicated significant difference in TEC and VPRC. For G3, TEC was significantly high on 250 days of pregnancy (p<0.05), day of calving (p<0.01) and on 30 days post-partum (p<0.01) compared to control. The VPRC values were significantly high (p<0.05) for G3 on day of calving compared to control. No significant differences observed on Hb concentration and TLC between supplemented and control group. Blood GSH-Px activity was significantly (p<0.05) high for G1 on day of calving and was significantly (p<0.05) high for G3 on 30 days post-partum. Serum catalase activity was significantly high for G0 on 30 days post-partum. Blood SOD does not show any significant difference in activity between supplemented and control animals. Serum MDA levels were significantly (p<0.01) different on 30 days post-partum with highest value shown by G0 and lowest value by G2. Within group between period analysis revealed significantly high MDA values on 30 day post-partum for G0. On the day of calving, serum total protein and globulin concentration were significantly (p<0.01) low for G3. No significant difference was observed in serum albumin, albumin : globulin ratio and serum selenium concentration. The results of the study revealed that dietary supplementation of Se @0.3 ppm and/or vitamin E @ 1000 IU could improve the antioxidant status in transition dairy cows significantly.