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Kerala Veterinary and Animal Sciences University, Wayanad

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Subject: Wildlife Studies × Author: Vishnu, O × Start date: 2020 ×
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    ThesisItemOpen Access
    ASSESSING GENETIC DIVERSITY OF TIGER (Panthera tigiris tigiris) IN WAYANAD WILDLIFE SANCTUARY BY USING NONINVASIVE TECHNOLOGIES
    (Kerala Veterinary and animal science university, Pookode, 42502) Vishnu, O; Arun Zachariah
    The Royal Bengal Tiger is the most charismatic and adaptable large cat species of India and holds about 70% of the world’s wild tiger population. This study aims to look into the genetic diversity of tigers in Wayanad Wildlife Sanctuary, which is an integral part of the largest tiger population in the world, by analyzing allelic diversity in six microsatellite loci by extracting DNA from the scat samples. In total sixty scat samples were collected from eighteen sampling blocks of four ranges from Wayanad Wildlife Sanctuary and DNA was extracted. All the samples were screened with the tiger specific cytochrome b primer set (TIF/TIR) for separating tiger scat from its sympatric carnivores. The forty percentage of the DNA isolated from the scat has been identified as of tiger origin. The samples positive for tiger DNA was amplified for the six microsatellite loci i.e. D10, E7, C34, E21, 3E6 and D15. The mean amplification rate and allelic dropout were observed to be 91% and nine percentage respectively. The observed heterozygosity and an expected heterozygosity were 0.48 and 0.72 respectively. The samples/ loci anallysed in the present study were deviated from Hardy Weinberg Equilibrium.