Thumbnail Image

Kerala Veterinary and Animal Sciences University, Wayanad

Browse

20221
Reset filters
Subject: Veterinary Public Health × Author: HEMA PERSIS ANDREWS × End date: 2023 × Start date: 2020 × Thesis Type: M.V.Sc. ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    OCCURRENCE OF CARBAPENEMASE AND EXTENDED SPECTRUM β-LACTAMASE PRODUCING MAJOR ENTEROPATHOGENS IN POULTRY FARMS OF WAYANAD
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE,WAYANAD, KERALA VETERINARY AND ANIMAL SCIENECES UNIVERSITY, 2022-09-22) HEMA PERSIS ANDREWS; Dr. Asha K
    Antimicrobial resistance is a global health emergency which leads to therapeutic failure of existing antibiotics and increases the health cost. The presence of antimicrobial resistant zoonotic pathogens such as E. coli and Salmonella spp. in broiler poultry and farm environments act as a potential source of transmission of AMR genes to humans which are of critical public health concern. Hence, the present study is envisaged to evaluate the occurrence of the above pathogens from organised broiler farms in the Wayanad district and to characterize the ESBL and carbapenem resistance of the recovered isolates. A total of 211 cloacal swabs from broiler chicken (189), Japanese quail (22) and 60 farm environmental samples were analysed for E. coli and Salmonella spp. by conventional microbiological, biochemical and molecular methods and the occurrence was observed as 65.68 and 14.76 per cent, respectively. Further, the characterization of isolates for ESBLproduction through the phenotypic disc diffusion method, confirmed 39.88 per cent of E. coli isolates and 25 per cent of Salmonella isolates as ESBL producers and PCR- basedgenotypic characterisation of ESBL revealed 73.59 per cent of E. coli and 57.5 per cent of Salmonella isolates were harbouring at least one of the selected ESBL genes i.e, blaCTX M, blaSHV and blaTEM. The ascending order of carbapenem resistance by the isolates upon phenotypic assay was Doripenem, Ertapenem, Meropenem and Imipenem for E. coliwhereas the the order was Doripenem, Ertapenem, Imipenem and Meropenem in case ofSalmonella isolates. None of the isolates was found positive for the carbapenem resistant genes blaNDM, blaOXA-48, blaIMP and blaVIM when screened by PCR. Thus, a diversephenotypic and genotypic pattern of ESBL and carbapenem resistance was observed among isolates. The present study envisages the occurrence of E. coli and Salmonella among broiler poultry and farm environment and the ability of these pathogens to harbour and disseminate ESBL and carbapenem resistance genes