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Kerala Veterinary and Animal Sciences University, Wayanad

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20192
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Subject: Veterinary Physiology × End date: 2019 × Start date: 2019 ×
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    ThesisItemOpen Access
    EXPRESSION OF GONADOTROPINS AND THEIR RECEPTORS IN ATTAPPADY BLACK AND MALABARI GOATS
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR,, 2019-12-31) BHUVANA PLAKKOT; K. Raji
    The difference in prolificacy between high prolific Malabari and low prolific Attappady Black goat breeds of Kerala has been evaluated by gonadotropins and their receptors gene expression studies, and analysis of steroid hormones. Six numbers of experimental animals, each from Malabari and Attappady Black breeds were selected for the study from the culling list of University Sheep and Goat Farm, Kerala Veterinary and Animal Sciences University, Mannuthy. Ovaries, pituitary and serum samples were collected from animals slaughtered at the proestrus stage of the oestrus cycle. By radioimmunoassay technique, a significant difference was observed in serum oestradiol concentrations, with Malabari having a higher mean (44.22±7.00 pg/mL) when compared to the Attappady Black breed (13.98±4.35pg/mL). The ovarian antral follicles were isolated and classified into small (1-3 mm) and large (>3 mm) follicles. RNA isolated from the follicles and pituitaries were used for expression studies. By performing quantitative real-time PCR technique, it was observed that between the breeds, there was no significant difference in the level of FSHβ expression in the pituitary, FSHR expression and LHR expression in follicles. However, there was a significant difference in the pituitary LHβ expression between breeds, with a significantly (P<0.01) high level in Attappady Black compared to Malabari breed. A non-significant decrease in (fold-change of 0.64) FSHR expression and a non-significant increase in (fold-change of 1.4) LHR expression were observed from small to large follicles in goats. Irrespective of breeds, a non-significant negative correlation (-0.49) was observed between oestradiol and FSHβ expression. Also, a non-significant negative correlation (-0.15) was observed between LHβ expression and progesterone concentration. A positive correlation although not very significant was found between FSHβ expression and FSHR expression in goats irrespective of breeds. Similarly, between FSHβ expression and LHR expression in goat antral follicles, a significant positive (P<0.05) correlation was observed. The antral follicle FSHR expression irrespective of breeds, showed a significant (P<0.05) positive correlation (0.46) with the oestradiol concentration. No correlation was found between pituitary overall LHβ and follicle LHR expression in antral follicles irrespective of the breed. A significant (P<0.05) positive correlation was also observed between oestradiol and oestrus signs and Malabari breed exhibited prominent oestrus signs compared to Attappady Black breed.
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    ThesisItemOpen Access
    EXPRESSION OF ANGIOPOIETIN-1 (ANPT-1) AND AROMATASE (CYP19) IN OVARIAN ANTRAL FOLLICLES OF MALABARI AND ATTAPPADY BLACK BREEDS OF GOATS.
    (College of Veterinary and animal Science,Mannuthy, 2019) SHIJI S. SAJU; V. Babitha
    The expressions of ANPT-1 and CYP19 genes in small and large follicles were studied in Malabari and Attappady Black breeds of goat. Animals (n=6) were selected from each breed and given PGF2α, after confirming oestrous by detecting visible external heat signs and open cervix using vaginal speculum, and ultra sound scanning was done in the animals for one entire cycle and observed the ovarian activities. We noticed a significant difference in the exhibition of heat signs between two breeds with a greater mean [(± S.E) 12±1.88] in Malabari than Attappady black breed [with a mean (± S.E.) 5.67 ±1.12]. On detecting the next heat, scanning was done from day 15 onwards and the animals were slaughtered on reaching pro-oestrous stage and the ovaries and blood samples were collected. Studied the expression pattern of ANPT-1 and CYP19 in different categories of ovarian antral follicles and estimated the serum oestradiol and progesterone concentration by RIA. Significant difference was found in serum oestradiol concentration between Malabari [mean (± S.E) 44.23 ± 7.00 pg/mL] and Attappady black breed [mean (± S.E) 13.95 ± 4.35 ng/mL], with no significant difference detected in serum progesterone concentration between breeds. The expression of ANPT-1 and CYP19 in follicles at different developmental stages within and between breeds failed to show any significant difference. The expression of ANPT1 was found to be decreased in large follicles of both breeds compared to small follicles whereas the expression of CYP19 was found to be increased in the large follicles of both breeds compared to small follicles. Hence it is concluded that changing pattern of ANPT-1 and CYP19 expression have a definite role in controlling the follicular development in these two breeds.