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Kerala Veterinary and Animal Sciences University, Wayanad

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20211
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Subject: Veterinary Pathology × Author: CHRISTY MARGRAT JOY × End date: 2023 × Start date: 2020 ×
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    ThesisItemOpen Access
    IMMUNOHISTOCHEMICAL ANALYSIS OF p70S6 KINASE PROTEIN IN MAMMARY TUMOURS OF DOGS
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSUR, 2021-08-30) CHRISTY MARGRAT JOY; Sajitha I.S
    The present study entitled “Immunohistochemical analysis of p70S6 kinase protein in mammary tumours of dogs” was conducted to analysis the gross features, classification and histopathology of canine mammary tumours. The study also envisaged for localization of p70S6kinase protein with respect to the grade of tumours. A total of 25 canine mammary tumour cases presented to the University Veterinary Hospitals at Mannuthy and Kokkalai during the period from March 2019 to March 2020 were included in the present study. Out of these, only one case was diagnosed as benign mammary tumour. A higher risk for mammary tumour occurrence was observed in dogs aged between 10-15 years (36 per cent) and 4-6 years (36 per cent) with a mean age of 8.04±0.60 years. Out of twenty five cases, only one case was reported in a male dog. Dachshund (24 per cent) represented the majority of the cases followed by Labrador (16 per cent) and Cross breed (16 per cent). Gross pathological features like shape, size, colour and consistency showed variations with a mean tumour size of 6.12±0.72 cm. Simple carcinomas were classified into ductal carcinoma, tubulopapillary carcinoma, solid carcinoma, comedocarcinoma, cribriform carcinoma and 65 per cent of them were either grade II or III whereas all the mixed tumours were classified as grade II. Immunohistochemistry was performed to study the expression of p70S6K protein in all the cases of canine mammary tumours. Twelve per cent (3/25) of the tumours were strongly positive (+++) and sixty per cent (15/25) were moderately positive (++). Rest twenty eight per cent (7 cases) have shown weak immunostaining (+). On statistical analysis, it was found that, there was correlation between the grade of the tumour and p70S6K expression (p<0.05). High grade malignant tumours showed strong to moderate expression of p70S6K protein. Benign mammary tumours in the present study were having weak expression of the protein. It has been reported that mTOR pathway and its downstream molecules including p70S6K are aberrantly expressed and have significant role in the progression of many human cancers. But no systematic studies are there with respect to canine mammary tumours. The present study thus is a preliminary work that could identify a novel biomarker protein in canine mammary tumours which could be used as a suitable target for diagnosis and therapy. A systematic study with higher sample size is warranted to identify the key regulators of the pathway in canine mammary tumours.