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Kerala Veterinary and Animal Sciences University, Wayanad

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20161
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Subject: Veterinary Microbiology × Author: RINSHA BALAN × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    MOLECULAR DETECTION OF ROTAVIRUS IN POULTRY OF KERALA
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCE-MANNUTHY,THRISSUR, 2016-06-30) RINSHA BALAN; MINI, M
    The present research was undertaken to detect the presence of rotavirus in faecal samples of birds with diarrhoea by RT-PCR. The RNA profile of the isolate was analysed by RNA-PAGE for grouping. One hundred and forty three faecal samples of poultry were collected from University poultry and duck farm, Kerala Veterinary and Animal Sciences University, Mannuthy, Poultry Farm under Instructional Livestock Farm Complex (ILFC), Pookode, four Regional poultry farms in Kerala and University Veterinary Hospital, Mannuthy. All the samples were screened for the presence of avian rotavirus by RT-PCR and RNA-PAGE and the samples have been studied with the focus on VP6 gene of rotavirus. Among 143 samples collected, five (3.49 per cent) were found to be positive by RNA-PAGE. In PAGE, the 11 segments of rota viral genome were observed revealing mammalian type group A rotavirus electropherotype (4:2:3:2). None of the samples displayed the avian type electropherotype A and D rotavirus. The Chi square test revealed that RT PCR was found to be more sensitive, specific and accurate than RNA-PAGE. Hence from the present study, RT-PCR could be recommended for screening of faecal samples suspected for avian rotavirus.