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Kerala Veterinary and Animal Sciences University, Wayanad

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Subject: Veterinary Microbiology × Author: DIWAKAR DATTATRAYRAO KULKARNI × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    ASSESSMENT OF IMMUNITY TO DUCK PLAGUE VIRUS (DUCK VIRUS ENTERlTlS) ON VACCINATION
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR, 1993) DIWAKAR DATTATRAYRAO KULKARNI; P.c. James
    During 1991, six outbreaks clinically duck plague (DP) with 33 per cent morbidity mortality were investigated. Duck plague from each outbreak. The isolates were able lesions and death of the duck embryos but fai chicken embryos during initial passages. suspected to be and 26 per cent was isolated to produce the led to kill the One of the strain, named DP-S was par by 10 passages in chicken embryos following duck embryos. Though the attenuated strain did pathogenicity index was reduced from 1.9 to 1. DP-S under transmission electron microscope of herpes virus morphology. Two DP vaccines - commercial vaccine vaccine having virus titres 0.74 and 3.5 respectively, were separately inoculated into ducklings respectively, two groups receiving two receiving double dose of corresponding interval of four weeks. Another group of duckl as control without vaccination. tially attenuated 20 passages in kill ducks, its 23. The isolate revealed virions and lab-adapted log 10 ELD 50/ml four groups of single dose and vaccines at an ings was kept (ii) challenged with Thre e ducks in each group were virulent DPV at four,eight and 20 weeks post-vaccination. The birds in all the five groups were screened at regular intervals for studying the immune response by virus neutralization (VN), leucocyte migration-inhibition (LMI) and passive haemagglutination (PHA) test. The challenged and survived birds were the carrier status of DPV by examination of their for virus isolation. In an organized farm, 180 ducks were gi vaccine at one year of age and were scre antibodies, LMI response and PHA titres before post-vaccination. Randomly selected two birds six weeks post-vaccination. The findings of the study are briefly l Six duck plague outbreaks were investigat isolated, and characterized. It was in duck and chicken embryos. partially The commercial vaccine could elicit very response as compared to laboratory adapted immunity could not last long even upto ei single vaccination and 20 weeks in double screened for rectal swabs ven commercial ened for VN and eight weeks were challenged isted as under: ed, the virus attenuated poor immune vaccine. The ght weeks in vaccination. (iii) * Single vaccination is not effective as c vaccination given four weeks apart. The assessment of antibody-mediated (AMI) and cell mediate d (CMI) immune responses indicated that both and CMI are involved in protection of ducks against duck plague. The vaccinated or vaccinated and infected show carrier status as attempts to isolate rectal swabs collected after vaccination were unsuccessful. The PHA has been standardized for diagnosis of duck plague.