Thumbnail Image

Kerala Veterinary and Animal Sciences University, Wayanad

Browse

20161
Reset filters
Subject: Veterinary Microbiology × Author: AISHWARYA J × End date: 2019 × Start date: 2010 ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    DETECTION AND MOLECULAR CHARACTERIZATION OF EMERGING VIRAL PATHOGENS OF PIGS
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD, 2016) AISHWARYA J; Chintu Ravishankar
    An array of viruses cause economically important diseases in pigs. The important viruses affecting pigs include classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), African swine fever virus, transmissible gastro enteritis virus, porcine circovirus 2 (PCV2), porcine parvovirus (PPV), and porcine rotavirus (PRV) to name few. A comprehensive study was done to detect the presence of CSFV, PCV2, PPV and PRV in pigs in Kerala. A total of 52 samples, collected from domestic and wild pigs of different districts of the State, were utilized for the study. Of these, 38 samples were tested for CSFV, PPV and PCV2. For CSFV detection nested reverse transcription polymerase chain reaction (RT PCR) targeting E2 gene region was carried out. Of the samples tested, five samples were detected as positive giving a percentage positivity of 13.56 per cent. These samples were from wild pigs. Sequencing and subsequent phylogenetic analysis of the isolates revealed that it belonged to serotype 2.2 which was having close sequence similarity to other Indian isolates and to isolates from Thailand. For PPV detection PCR targeting NS1 gene region was done and two samples were detected as positive with percentage positivity of 5.26. Of the two positive samples, one was from a wild pig. For PCV2 detection PCR and TaqMan real time PCR targeting ORF2 gene were carried out. Only two samples were detected as positive in PCR while real time PCR detected 15 samples (39.47 per cent) as positive. In one of the samples, mixed infection with PCV2 and PPV was detected. On phylogenetic analysis, isolates of PCV2 and PPV were found to be more similar to Chinese strains indicating a probable entry of these viruses from that country. For PRV detection RT-PCR targeting Seg 6 gene was carried out and none of the 14 samples were detected as positive. Attempts were carried out to isolate CSFV, PPV and PCV2 in PK-15 cell lines. The presence of the virus in the infected cells could not be detected by RT PCR and direct immunofluorescence test for CSFV and by PCR for PCV2 and PPV after three blind passages.