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Kerala Veterinary and Animal Sciences University, Wayanad

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20161
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Subject: Veterinary Gynaecology and Obstetrics × Author: ALANTEENA, JACOB × End date: 2019 × Start date: 2015 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    IN VITRO FERTILITY ASSESSMENT OF FROZEN BOVINE SPERMATOZOA AS INFLUENCED BY BREED AND SEASON
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES-MANNUTHY,THRISSUR, 2016) ALANTEENA, JACOB; Metilda Joseph
    Frozen semen of four animals of each breed- crossbred Holstein Friesian (CBHF- Holstein Friesian X Gir), crossbred Jersey (CBJY- Jersey X Red Sindhi), Vechur (VR) and Kasargod Dwarf (KD)- produced from August 2015 to May 2016 during South west monsoon, Post-monsoon, Winter and Summer were procured from semen bank of KLDB, Dhoni. Minimum two samples of each bull (n=16) processed during each season were subjected to different in vitro fertility tests like routine semen quality tests (post-thaw motility, viability and abnormality), sperm function tests (acrosome integrity and hypo-osmotic swelling test), fertility tests (bovine cervical mucus penetration test and in vitro capacitation test with heparin). In vitro capacitation was assessed indirectly by inducing acrosome reaction using Lysophosphatidyl choline and it was evaluated by Trypan Blue-Giemsa staining. Statistical analysis of collected data revealed that season had significant (P<0.05) effect on post-thaw motility, sperm abnormality, response to hypoosmotic swelling and induced acrosome reaction whereas, breed had significant (P<0.05) effect only on post-thaw motility and bovine cervical mucus penetration distance of frozen bull semen. Among four seasons, winter was found to be better for frozen semen production and among breeds CBHF and KD semen exhibited better frozen semen quality than that of other two breeds. The crossbred semen samples studied did not show any significant reduction in quality during summer season in midlands of Kerala and also they showed better performance during winter compared to the indigenous cattle. This may be attributed to the combination of genetic makeup in the crossbreds. But during humid seasons, monsoon and post monsoon, frozen semen of indigenous bulls were better in quality. The results of present study indicate that crossbreeding policy of cattle in Kerala can be continued by employing the most adaptable superior bulls along with the conservation of indigenous animals to overcome the effects of global warming. Also distribution of frozen semen can be reviewed with alterations in supply chain by substituting quality semen produced during favourable seasons for AI during adverse climatic conditions based on the geographic location to enhance the fertility in female animals.