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Kerala Veterinary and Animal Sciences University, Wayanad

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20171
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Subject: Animal Reproduction, Gynaecology and Obstetrics × Author: AKHILA V V × End date: 2023 ×
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    ThesisItemOpen Access
    FREEZABILITY OF MALABARI BUCK SEMEN SUPPLEMENTED WITH CURCUMIN AND ASCORBIC ACID
    (COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD, 2017) AKHILA V V; K. Promod
    The aim of this study was to determine the cryopreservation induced oxidative damages and the effects of curcumin and ascorbic acid supplementation on post thaw quality of Malabari buck semen. Sixty ejaculates were collected from five adult Malabari buck were used for the study. After removal of seminal plasma, the sperm pellets in Tris buffer were pooled and then divided into five equal parts. The semen sample belonging to C-1 and C-2 were extended with Tris based extender containing 0.5 mM and 2.5 mM curcumin, respectively. Semen samples of A-1 and A-2 were extended with Tris based extender containing 2.5 mg/ml and 8.5 mg/ml ascorbic acid, respectively and the final group without antioxidants as control. The extended semen was packed in French mini straws and manual freezing was carried out after an equilibration period of two hours at 5°C. The pre freeze motility and viability was found to significantly (p<0.01) higher in groups with 0.5 mM curcumin and 2.5 mg/ml ascorbic acid than that of control. There was no significant difference (p>0.01) in sperm morphological abnormalities, malondialdehyde concentration and DNA integrity between the groups. A significantly (p<0.01) higher percentage of acrosome integrity was observed with 2.5 mg/ml ascorbic acid supplementation. The functional membrane integrity was significantly higher in groups with 2.5 mM curcumin and 2.5 mg/ml ascorbic acid. The supplementation of 0.5 mM curcumin and 2.5 mg/ml ascorbic acid resulted in significantly (p<0.01) higher post thaw motility and viability. The post thaw sperm abnormality per cent with supplementation of 8.5mg/ml ascorbic acid was significantly (p<0.01) higher than that of other treatment groups and control. The groups containing 2.5 mM curcumin and 2.5 mg/ml ascorbic acid showed significantly (p<0.01) higher functional membrane integrity. Acrosome integrity was found to be significantly (p<0.01) higher with 2.5 mg/ml ascorbic acid supplementation whereas 2.5 mM curcumin supplementation significantly reduced the malondialdehyde production than the other groups. There was no significant difference (p>0.01) in the DNA integrity with supplementation of antioxidants than that of control. Thus supplementation of 2.5 mg/ml ascorbic acid resulted in better post thaw sperm quality. The two curcumin supplemented groups showed different effect on the sperm parameters under study. Further in vivo fertility trial are required before recommending 2.5 mg/ml ascorbic acid for field level artificial insemination.