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University of Agricultural Sciences, Dharwad

The University of Agricultural Sciences, Dharwad was established on October 1, 1986. The University has 5 Colleges, 27 Research Stations, 6 Agriculture Extension Education Centers, 6 Krishi Vigyan Kendras and ATIC. The University has its jurisdiction over 7 districts namely Bagalkot, Belgaum, Bijapur, Dharwad, Gadag, Haveri, and Uttar Kannada in northern Karnataka. Greater diversity exists in soil types, climate, topography cropping and farming situations. The jurisdiction includes dry-farming to heavy rainfall and irrigated area. Important crops of the region include sorghum, cotton, rice, pulses, chilli, sugarcane, groundnut, sunflower, wheat, safflower etc. The region is also known for many horticultural crops. Considerable progress has been registered in the field of education, research and extension from this University.

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20131
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Subject: Molecular Biology and Biotechnology × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Induced systemic resistance and validation of post transcriptional gene silencing in tomato against tomato leaf curl virus
    (UAS Dharwad, 2013) Sarita V. Gund; Krishnaraj P. U.
    The efficacy of transgenic plants capable of PTGS was assessed in available T4 seeds of four transgenic events viz., A, C, D and E carrying ihp-TRP subjected for PCR based screening and gene segregation analysis. The bio-efficacy test of transgenic plants from three events viz., ‘A’,’C’ and ‘E’ in T4 and T5 generation indicated moderate resistance against ToLCV infection. The site of insertion of T-DNA carrying ihp-TRP genes was identified by recovering the genomic sequences flanking right border of T-DNA obtained from TAILPCR, identified the point of insertion on chromosome 1, chromosome 12 and chromosome 2 for ‘A’, ‘C’ and ‘E’ events respectively. Thirty Pseudomonas and five actinobacteria isolates were functionally characterized for ability of phosphate solubilization, nitrogen fixation, siderophore, indole-acetic acid and gibberellic acid production. Out of thirty five, twenty isolates were prospected for control of tomato leaf curl viral disease through seed priming, soil and foliar application to tomato plants. Inoculation of five isolates, viz., AUDP326(4), AUDP360(2), AUDP139, AUDT217 and AUDT152 resulted in significant reduction in disease up to 60-80% and also could significantly promote plant growth. Significant increase in the level of PAL, PO, chitinase and phenolics were observed in inoculated plants compared to disease control. The SSH studies at early (32-36 DAS) and late stage (40-46 DAS) in AUDT217 inoculated tomato plants compared to uninoculated plants identified sixteen well annotated genes such as protease inhibitor, threonine deaminase and other. However, SSH studies involving inoculation of AUDT217 in the presence of ToLCV realized in the identification of twelve well annotated transcripts of genes in the plant such as metallothionein-like protein, ferredoxin-thioredoxin-reductase, metallocarboxypeptidase inhibitor and other which were further validated through real time PCR studies.