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Bihar Agricultural University, Sabour

Bihar Agricultural University, Sabour established on 5th August, 2010 is a basic and strategic institution supporting more than 500 researchers and educationist towards imparting education at graduate and post graduate level, conducting basic, strategic, applied and adaptive research activities, ensuring effective transfer of technologies and capacity building of farmers and extension personnel. The university has 6 colleges (5 Agriculture and 1 Horticulture) and 12 research stations spread in 3 agro-ecological zones of Bihar. The University also has 21 KVKS established in 20 of the 25 districts falling under the jurisdiction of the University. The degree programmes of the university and its colleges have been accredited by ICAR in 2015-16. The university is also an ISO 9000:2008 certified organisation with International standard operating protocols for maintaining highest standards in teaching, research, extension and training.VisionThe Bihar Agricultural University was established with the objective of improving quality of life of people of state especially famers constituting more than two third of the population. Having set ultimate goal of benefitting society at large, the university intends to achieve it by imparting word-class need based agricultural education, research, extension and public service.

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20191
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Subject: Plant Breeding × Author: Kumar, Rishav × End date: 2019 × Start date: 2019 ×
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    ThesisItemOpen Access
    Marker assisted introgression of a major QTL (qDTY1.1) for grain yield under drought at reproductive stage in Oryza sativa L. cv. Sita
    (Department of Plant Breeding and Genetics, BAU, Sabour, 2019-02-11) Kumar, Rishav; Singh, P. K.
    Rice is the second largest crop in the world in terms of area and production which is grown in all the continents except Antartica. It is the primary source of food, nutrients, energy and provides employment to more than 3.5 billion people. But being one of the staple food crops, it is also affected by biotic and abiotic stress. With the change in environmental condition, drought is emerging as one of the major threats to rice. As per India Meteorological Department, there was an early departure of monsoon in 2018 which resulted in failure of Hathiya Nakshtra, causing drought coincide with the reproductive stage. Last but not the least is the susceptibility of most of the mega varieties of rice like Sita during reproductive stage. Hence, there is need to improve such varieties which can cope up such harsh period. Previously, conventional methods were used for improvement of crop variety but it takes a lot of time. With the advancement in the field of molecular biology, MAS acts as a milestone in this field. Now with the discovery of QTLs efficiency of MABB has surpassed the conventional breeding. With this view, marker assisted introgression of a major QTL (qDTY1.1) for grain yield under drought at reproductive stage in Oryza sativa L. cv. Sita has been taken under consideration. To incorporate drought tolerance, the variety ‘Sita’ was introgressed with QTL qDTY1.1 from Nagina-22. The present research work started from Kharif 2015 and Sita and Nagina-22 were sown for availability of pollens. Twenty five F1 plants obtained were sown in Kharif 2016 and validated with RM431 which identified ten heterozygous plants were identified on which further backcrossing was done with Sita to obtain sixty seven BC2F1 plants that were sown in Kharif 2017 and validated with RM431. A total of sixty seven heterozygous plants were obtained which were again backcrossed with Sita to produce 179 BC2F1 plants. Through foreground selected marker RM431 identified 98 plants which were found heterozygous. The selected plants were subjected to recombinant selection with RM3825 and RM12091. RM3825 identified three plants (plant number 23, 65, 94) sowing band size similar to Sita (130 bp), while through RM12091 a total of six recombinant plants were identified (plant number 23, 63, 65, 94, 97 and 119). Three plants were common when checked with both the recombinant markers. So, six recombinant plants (plant number 23, 63, 65, 94, 97, 119) were selected for carrying out background selection with 49 polymorphic markers. Recipient allele percentage ranged from 69.38 to 79.59%. Plant number 119 showed maximum background recovery (79.59%) followed by plant number 94, 97, 65 and 23 with 77.55%, 75.51%, 74.48%, 73.46% recovery respectively. Plant number 63 exhibited minimum recovery (69.38%). Morphological observations (days to anthesis, plant height, panicle length, number of panicles, grains per panicle and grain yield per plant) were taken during drought and it was found that recombinants obtained were superior with respect to recurrent parent Sita for all the characters. Biochemical observations (proline content, relative water content and total chlorophyll content) also revealed that recombinants were superior with respect to recurrent parent. On the basis of molecular, morphological and biochemical observations the plant number 119 should be carried forward for further backcrossing to improve its recipient allele percentage.