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Bihar Agricultural University, Sabour

Bihar Agricultural University, Sabour established on 5th August, 2010 is a basic and strategic institution supporting more than 500 researchers and educationist towards imparting education at graduate and post graduate level, conducting basic, strategic, applied and adaptive research activities, ensuring effective transfer of technologies and capacity building of farmers and extension personnel. The university has 6 colleges (5 Agriculture and 1 Horticulture) and 12 research stations spread in 3 agro-ecological zones of Bihar. The University also has 21 KVKS established in 20 of the 25 districts falling under the jurisdiction of the University. The degree programmes of the university and its colleges have been accredited by ICAR in 2015-16. The university is also an ISO 9000:2008 certified organisation with International standard operating protocols for maintaining highest standards in teaching, research, extension and training.VisionThe Bihar Agricultural University was established with the objective of improving quality of life of people of state especially famers constituting more than two third of the population. Having set ultimate goal of benefitting society at large, the university intends to achieve it by imparting word-class need based agricultural education, research, extension and public service.

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2018 - 201912020 - 20223
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Subject: Genetics and Plant Breeding × Type: Thesis ×
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Now showing 1 - 4 of 4
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    ThesisItemOpen Access
    Genetic study for grain yield quality traits in maize (Zea mays L.)
    (Department of Plant Breeding and Genetics, BAU, Sabour, 2020) Rishavnandan; Rashmi, Kumari
    The present study was aimed to assess the general combining ability of parents and specific combining ability of their crosses for yield and yield related traits. Nine inbred lines were crossed with two testers using line × tester mating design. Nine lines, two testers and 18 experimental hybrids were evaluated alongwith two checks in this study for grain yield and protein content, besides kernel micronutrients (Phosphorous, Calcium and Iron) during kharif 2019 in a randomized block design at experimental farm of BAC, Sabour.The parents CLQRCY44 and HKI1532 were found as good general combiner for grain yield & quality traits followed by DHOLI Inbred 55 and CLO2450. Parents DHOLI Inbred 55 and CML451 were found as good general combiner for earliness. Parents CLQRCY44, CLO2450 & DHOLI 55 were found as good general combiner for quality traits like protein content, calcium content, phosphorous and iron content. Among the crosses, HKI1532 x 95IOWA (67.43q/ha), LM13 x SUWAN (65.87q/ha) and CML425 x SUWAN (63.30q/ha) were the promising crosses for grain yield. CLO2450 x SUWAN (7.77) and LM13 x SUWAN (6.77) exhibited highly significant and desirable SCA effects for grain yield. CLO2450 x 95IOWA (0.53) exhibited highly significant and desirable SCA effects for protein content. HKI1532 x 95IOWA (67.43q/ha) was the promising hybrid for grain yield and micronutrient content. Therefore, these crosses can be utilized for developing high yielding micronutrient enriched hybrid varieties in maize.
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    ThesisItemOpen Access
    Genetic Studies on Quality Protein Maize (Zea mays L.)
    (Department of Plant Breeding and Genetics, BAU, Sabour, 2022) Swapnil; Rashmi, Kumari
    An investigation was carried out with fourteen inbred lines (females) and three testers (male) and these were crossed to produce 42 F1s using the line x tester mating design during rabi, 2020-21. In kharif 2021, 61 genotypes (42 crosses, 14 lines, 3 testers and 2 checks) were evaluated in Randomized Block Design with three replications in three different environments i.e., pre-kharif (date of sowing: 15th May)- E1, kharif (date of sowing: 30th June)- E2, post-kharif (date of sowing: 15th August)- E3. The data were recorded for eighteen characters viz., days to 50% tasseling, days to 50% silking, anthesis silking interval, days to 75% brown husk, plant height, ear height, kernel rows per ear, kernels per row, 1000- grain weight, cob length, cob girth, grain yield (kg ha-1), kernel starch content (%), kernel oil content (%), kernel protein content (%), tryptophan content (%), lysine content (%) and kernel vitreosity (%) to study genetic variability, correlation coefficient analysis, path coefficient analysis, combining ability, heterosis and stability analysis for yield and yield attributing traits. High genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) were recorded for traits viz., 1000- grain weight and grain yield which reveals the presence of a higher magnitude of variability in the population for these traits. High heritability accompanied by high genetic advance was recorded for the characters viz., ear height, kernel row per ear, kernels per row, 1000- grain weight, cob length and grain yield. These traits are governed by additive genes and selection may be rewarding for the improvement of such traits. Correlation coefficient analysis revealed that the traits viz., ear height, kernel rows per ear, kernels per row, 1000- grain weight, cob length, cob girth, and kernel vitreosity displayed a positive and highly significant correlation with grain yield at both genotypic and phenotypic levels. The positive correlation between the desirable characters leads to simultaneous improvement of both the characters. High direct effect on grain yield was recorded for traits viz., ear height, kernel rows per ear, kernels per row, 1000- grain weight and cob length. The direct selection of these traits will be rewarding for yield improvement. The pooled analysis of variance for the design of experiment over three environments revealed highly significant mean squares due to environments, genotypes and genotype x environment interactions for all the traits under study. The promising experimental hybrids with high sca and high gca effects were L2 x T2, L5 x T3, L6 x T3, L8 x T2, L10 x T3 and L12 x T3 for both the parents for grain yield and the hybrids L5 x T1, L6 x T1, L11 x T1 and L12 x T1 had high sca and high gca effects for at least female parent. Sca variances were higher than the gca variances for all the traits under study which depicted the preponderance of non-additive gene action in the inheritance of these traits. The five best hybrids viz., L14 x T3, L12 x T1, L5 x T1, L7 x T3 and L5 x T2 were identified on the basis of high sca effects as well as high economic heterosis on the pooled basis over the best check, HQPM-5 for grain yield. The estimates of stability parameters for grain yield revealed seven hybrids, L5 x T3, L6 x T2, L6 x T3, L7 x T1, L13 x T3, L14 x T1, L14 x T2 showed non-significant (S2di), high mean value than the population mean and regression coefficient around unity (bi≃1). It revealed the stability of this hybrid for high grain yield under varying environmental conditions Hence, it is suggested from this study that the parents identified could be utilized for the development of either the synthetic varieties or elite breeding population and the crosses identified could be directly used for heterosis breeding in maize.
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    ThesisItemOpen Access
    Effect of terminal heat stress on genetic responses of biochemical and yield contributing parameters in wheat (Triticum aestivum L.)
    (Department of Plant Breeding and Genetics, BAU, Sabour, 2021) Rashmi, Deep; De, Nitish
    Heat stress is a matter of a great concern for the wheat crop. Breeding of heat-tolerant cultivars requires knowledge of the genetic behaviour of morpho-physiological traits. Genetic analysis through Generation Mean Analysis helps to find out the nature of gene actions involved in a concerned trait by providing an estimate of main gene effects (additive and dominance) along with their digenic interactions (additive × additive, additive × dominance, and dominance × dominance. Thus, six generations (P1, P2, F1, BC1.1, BC1.2 and F2) of six crosses were sown in timely (normal sown, NS) and late (heat stress, LS) conditions in a Randomized Block Design with three replications in each. Result of scaling test revealed that additive-dominance model is inadequate for explaining the inheritance of most of the studied characters under both environments indicating the presence of non-allelic gene interaction. The traits i.e., Grain filling duration (GFD), canopy temperature (CT), chlorophyll content (CC) and catalase activity (CAT) under heat stress condition was found under the control of additive gene action with dominance x dominance interaction, additive gene action with additive x additive interaction, dominance gene action with additive x additive interaction and dominance gene action with additive x additive and dominance x dominance interaction respectively. Heritability estimates in broad senses were generally moderate to high for most studied characters in six crosses except for grain yield and chlorophyll content. The expected genetic advance from selection estimates in the F2 were high for CT, MSI, CAT, S/MS and 1000GW. Significant genotypic correlation coefficients appeared among grain yield, canopy temperature, relative water content, chlorophyll content and catalase activity under late sown conditions. This suggests useful indirect selection criteria to improve simultaneously these traits, which showed significant heritability along with genetic advance. Moreover, canopy temperature can be considered a significant trait to indicate relative tolerance to terminal heat stress. It can be concluded that the traits investigated showed a complex genetic behaviour, which implies that early selection would be less efficient; therefore, it is recommended delaying the selection to advanced generations to benefit from the reduction of non-fixable genetic variation and exploit transgressive segregants.
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    ThesisItemOpen Access
    Identification and functional analysis of calmodulin binding protein gene(s) in disease resistance responses in rice (Oryza sativa L.)
    (Department of Molecular Biology and Genetic Engineering, BAU, Sabour, 2018-06) Kumari, Diksha; Prasad, Bishun Deo
    Plants are usually subjected to one or more biotic stresses which reduce agricultural output significantly, leading to a huge yield-gap. To cope with the various stimuli generated by various environmental stresses, plants have evolved with complex mechanism of signal perception and transduction. Several phytohormones and secondary messengers are the main players in mediating cellular responses to various stimuli. Of the various secondary messengers in eukaryotes, the role of Ca2+ has been most extensively studied. The calcium ion (Ca2+) is a well-known universal second messenger in plants regulating the responses of growth and development as well as different environmental stimuli in plant. Changing cytosolic-free calcium concentration ([Ca2+]cyt) is one of the earliest responses to biotic stresses. These changes in cellular Ca2+ level are being mediated by different Ca2+ binding proteins like calmodulin (CaM). CaM interacts with calmodulin binding protein (CBP) and activates downstream defense response. CBP is family of protein, among them, CBP60g also known as SARD1 (systemic acquired resistance deficient 1) is well characterized which involved in defense responses via systemic acquired resistance (SAR). However, no rice CBP60 (OsCBP60) has been identified in relation to pathogen infection till date. In this study we have identified 15 OsCBP60 gene using bioinformatics studies. The expression of different OsCDB60s were analysed in Xanthomonas oryzae pv. oryzae and Magnaporthe oryzae treated rice samples. We analysed the expression of OsCBP60s in defence hormone (Salicylic acid, Jasmonic acid and Brassinosterois) treatment in rice. Our studies showed that 3 OsCBP60s (LOC_Os09g13890, LOC_Os11g44680, LOC_Os03g32160) genes were upregulated consistently in all the time point studied after pathogen treatment. Two genes (LOC_Os11g44680 and LOC_Os12g36910) were found to be upregulated consistently in SA and EBR treated sample. Most of OsCBP60s genes were downregulated in JA treated sample. Interestingly, LOC_Os12g36910 was consistently upregulated in all after hormone treatment. Further, a candidate gene (LOC_Os11g44680) was cloned in cloning vector pJET1.2.