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Bihar Agricultural University, Sabour

Bihar Agricultural University, Sabour established on 5th August, 2010 is a basic and strategic institution supporting more than 500 researchers and educationist towards imparting education at graduate and post graduate level, conducting basic, strategic, applied and adaptive research activities, ensuring effective transfer of technologies and capacity building of farmers and extension personnel. The university has 6 colleges (5 Agriculture and 1 Horticulture) and 12 research stations spread in 3 agro-ecological zones of Bihar. The University also has 21 KVKS established in 20 of the 25 districts falling under the jurisdiction of the University. The degree programmes of the university and its colleges have been accredited by ICAR in 2015-16. The university is also an ISO 9000:2008 certified organisation with International standard operating protocols for maintaining highest standards in teaching, research, extension and training.VisionThe Bihar Agricultural University was established with the objective of improving quality of life of people of state especially famers constituting more than two third of the population. Having set ultimate goal of benefitting society at large, the university intends to achieve it by imparting word-class need based agricultural education, research, extension and public service.

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20181
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Subject: Genetics and Plant Breeding × End date: 2019 × Start date: 2010 ×
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    ThesisItemOpen Access
    Identification and functional analysis of calmodulin binding protein gene(s) in disease resistance responses in rice (Oryza sativa L.)
    (Department of Molecular Biology and Genetic Engineering, BAU, Sabour, 2018-06) Kumari, Diksha; Prasad, Bishun Deo
    Plants are usually subjected to one or more biotic stresses which reduce agricultural output significantly, leading to a huge yield-gap. To cope with the various stimuli generated by various environmental stresses, plants have evolved with complex mechanism of signal perception and transduction. Several phytohormones and secondary messengers are the main players in mediating cellular responses to various stimuli. Of the various secondary messengers in eukaryotes, the role of Ca2+ has been most extensively studied. The calcium ion (Ca2+) is a well-known universal second messenger in plants regulating the responses of growth and development as well as different environmental stimuli in plant. Changing cytosolic-free calcium concentration ([Ca2+]cyt) is one of the earliest responses to biotic stresses. These changes in cellular Ca2+ level are being mediated by different Ca2+ binding proteins like calmodulin (CaM). CaM interacts with calmodulin binding protein (CBP) and activates downstream defense response. CBP is family of protein, among them, CBP60g also known as SARD1 (systemic acquired resistance deficient 1) is well characterized which involved in defense responses via systemic acquired resistance (SAR). However, no rice CBP60 (OsCBP60) has been identified in relation to pathogen infection till date. In this study we have identified 15 OsCBP60 gene using bioinformatics studies. The expression of different OsCDB60s were analysed in Xanthomonas oryzae pv. oryzae and Magnaporthe oryzae treated rice samples. We analysed the expression of OsCBP60s in defence hormone (Salicylic acid, Jasmonic acid and Brassinosterois) treatment in rice. Our studies showed that 3 OsCBP60s (LOC_Os09g13890, LOC_Os11g44680, LOC_Os03g32160) genes were upregulated consistently in all the time point studied after pathogen treatment. Two genes (LOC_Os11g44680 and LOC_Os12g36910) were found to be upregulated consistently in SA and EBR treated sample. Most of OsCBP60s genes were downregulated in JA treated sample. Interestingly, LOC_Os12g36910 was consistently upregulated in all after hormone treatment. Further, a candidate gene (LOC_Os11g44680) was cloned in cloning vector pJET1.2.