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Bihar Agricultural University, Sabour

Bihar Agricultural University, Sabour established on 5th August, 2010 is a basic and strategic institution supporting more than 500 researchers and educationist towards imparting education at graduate and post graduate level, conducting basic, strategic, applied and adaptive research activities, ensuring effective transfer of technologies and capacity building of farmers and extension personnel. The university has 6 colleges (5 Agriculture and 1 Horticulture) and 12 research stations spread in 3 agro-ecological zones of Bihar. The University also has 21 KVKS established in 20 of the 25 districts falling under the jurisdiction of the University. The degree programmes of the university and its colleges have been accredited by ICAR in 2015-16. The university is also an ISO 9000:2008 certified organisation with International standard operating protocols for maintaining highest standards in teaching, research, extension and training.VisionThe Bihar Agricultural University was established with the objective of improving quality of life of people of state especially famers constituting more than two third of the population. Having set ultimate goal of benefitting society at large, the university intends to achieve it by imparting word-class need based agricultural education, research, extension and public service.

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2017 - 201912020 - 20211
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Subject: Fruit Science ×
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    ThesisItemOpen Access
    In vitro mutagenesis in banana (Musa paradisiaca L.) cv. Grand Naine for its improvement against salinity
    (Department of Horticulture (Fruit Science), BAU, Sabour, 2021-01) Rajan, Rajni; Ahmad, Mohammad Feza
    The present investigation entitled “In vitro mutagenesis in banana (Musa paradisiaca L.) cv. Grand Naine for its improvement against salinity” was carried out in the Division of Horticulture (Fruits and Fruit Technology), BAU, Sabour during 2017-2020 with the aim to develop salinity tolerant mutant in banana cv. Grand Naine using gamma irradiation and chemical mutagens. Six different experiments were laid out under in vitro and in vivo condition. The irradiation experiment was carried out in 5×5 factorial complete randomized design consisting days of subculture (DAS) (4 DAS, 7 DAS, 10 DAS, 13 DAS and 16 DAS) and irradiation doses (0 Gy, 10 Gy, 20 Gy, 30 Gy and 40 Gy) replicated five times. The chemical mutagen experiment was conceded in 5×7 factorial completely randomized design comprising days of subculture (4 DAS, 7 DAS, 10 DAS, 13 DAS, 16 DAS) and chemical mutagen doses (Control @ 0 mM, EMS @ 3 mM, EMS @ 5mM, DES @ 3 mM, DES @ 3 mM, NaN3 @ 1mM, NaN3 @ 2 mM) replicated three times. The observations with regards morphological characters were taken up to M1V4 generation (up to 120 days from M1V1 to M1V4 at 30 days interval). Under in vivo condition, ten mutant lines (SM1, SM2, SM3, SM4, SM5, SM6, SM7, SM8, SM9, SM10, SM11 and SM12) were used for evaluating salinity tolerance with the application of NaCl (0, 100 and 250 mM) under factorial completely randomized design. Results revealed that 39.5 Gy as the LD50 dose among gamma irradiated shoot tips. 10 Gy treated over 16 DAS explants was found most effective in terms of number of new shoots (8.86), survival (80-90 %), shoot length (5.54 cm), root length (5.44 cm). The LD50 of EMS, DES and NaN3 for shoot tip culture of banana was recorded at 5.5, 5.1 and 2.1 mM respectively. EMS and DES retained dormant condition until M1V2. EMS was found most effective in new shoot development. Morphological characters like shoot length (4.40 cm) and root length (5.30 cm) found maximum in NaN3 @ 1 mM and DES @ 3 mM respectively. All the morphological characters increased with increase in DAS. Explant of 16 DAS exposed with EMS @ 3mM improved survival rate in the range of 60-90 % and rooting by 70-75 %. EMS @ 3mM and EMS @ 5 mM performed best in the development of visible mutant when subculture was done at 10 and 13 DAS. As many as seven microsatellite markers were selected for identification of salinity, three have been found most suitable for screening of mutant tolerant to salinity. Ma2/3 showed no amplification whereas Ma1/3 and STMS 1fp/1rp showed amplification at 160 bp and 250 bp were selected for screening of salinity. 10 Gy when exposed over 13 DAS explants distinguished positive for markers whereas higher doses i.e. 20, 30 and 40 Gy were found screened positive at 4 DAS and 7 DAS. EMS @ 3 mM, EMS @ 5 mM and DES @ 5 mM at the 4, 7 and 10 DAS was found positive for salinity markers while NaN3 generated explants didn’t identified salinity tolerant line using molecular markers. There was no evidence of salinity positive lines in all the treatments at above13 DAS. Out of 1100 putative mutant line derived, 134 lines confirmed through molecular markers. Out of molecularly confirmed line 22 mutant lines were found effective based on survival, plant height and root length for further screening under in vitro salinity condition. The 12 most effectively performed lines were again screened for in vivo salinity experiments. Under in vivo condition among irradiated mutant lines SM1 and SM2 obtained from 10 Gy and 20 Gy and chemical mutagen treated lines SM7 and SM8 obtained from EMS @ 3 mM, EMS @ 5 mM respectively were found most effective treatments among mutant lines with respect to per cent change in plant height, leaf area, necrosis, number of leaves, MSI, RWC and proline. SM11 and SM12 obtained from treatment of NaN3 @ 1 mM and NaN3 @ 2 mM performed higher in terms of per cent change in salinity damage, chlorophyll, MSI and proline. SM9 (DES @ 3 mM) showed the improved per cent change in no. of leaves, proline and chlorophyll then other mutant lines. The entire set of experiment suggested that under in vitro condition lower gamma irradiation doses (10 Gy, 20 Gy) exposed over 13 DAS explants was found to be most effective to generate salinity tolerant mutant lines. Among chemical mutagens, EMS provided maximum number of visible mutant and salinity tolerant mutant line from 4 to 10 DAS. Under in vivo condition, 10 Gy and 20 Gy derived mutant lines were most effective to improve plant height, leaf area, necrosis, no. of leaves, MSI, RWC and proline under salinity condition. However, EMS @ 3 mM was found effective in improving plant height, leaf area, no. of leaves, MSI and proline. Also, DES @ 3 mM improved RWC and NaN3 @ 1 mM enhanced chlorophyll content of the mutant lines against salinity.
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    ThesisItemOpen Access
    Comparative performance of different genotypes of peach based on their rooting response of hardwood cuttings
    (Department of Horticulture (Fruit & Fruit Technology), BAU, Sabour, 2017-07) Kumar, Shashank; Prasad, Muneshwar
    Considering the unavailability of information of the effect of rooting hormones in combination with modified growing conditions on the rooting characteristics of Peach (Prunus persica (L.) Batsch) cuttings under agro climate conditions of Bihar, the experiment was undertaken at the Horticulture Garden, Bihar Agricultural University, Sabour, Bhagalpur, India. Besides being cultivated in the temperate climate in the hills, it is also cultivated in the North Indian plains (subtropical climate) in the states of Punjab, Haryana, Rajasthan and Uttar Pradesh with the introduction of low chilled peaches. The subtropical peaches come in the market early in season (mid-April), growers can get higher returns due to scarcity of other fresh fruits. As some varieties of Peach (Shan-i-Punjab, Pratap, Prabhat, Florda Prince & Early Grande) are available in Bihar and these are well responding in terms of fruiting. But its cultivation is limited in Bihar due to lack of its planting material. Properly prepared hardwood cuttings of about 15-20cm length and 0.8-1.2cm diameter having 5-6 buds were prepared from the dormant twigs of the 4-5 years old peach plants in the second fortnight of November. The basal portion of the cuttings was dipped in different doses of Nine treatments viz. IBA (1500 ppm, and 3000 ppm), NAA (1000 ppm, and 2000 ppm) and combinations of IBA and NAA (IBA 1500 ppm + NAA 1000 ppm, IBA 1500 ppm + NAA 2000 ppm, IBA 3000 ppm + NAA 1000 ppm and IBA 3000 ppm + NAA 2000 ppm) and control were evaluated. On the basis of data recorded on rooting parameters after 45 days of planting, it is concluded that highest survival percentage (90.63%) was recorded in Shan-e-Punjab followed by Early Grande (86.16%) and then by Florda Prince (80.54%), Pratap (72.35%), and Prabhat (40.62%) by IBA-3000ppm. Highest rooting percentage (60.12%) was recorded in Shan-e-Punjab followed by Early Grande (58.86%) and then by Florda Prince (56.32%), Pratap (43.87%), and Prabhat (31.12%) treated with IBA-3000ppm. Highest average root length (9.48cm) was recorded in Early Grande followed by Shan-e-Punjab (8.51cm), Florda Prince (8.23cm), Pratap (5.00cm) and Prabhat (4.55cm) by IBA-3000ppm. Highest root thickness (3.08mm) was recorded in Early Grande followed by Shan-e-Punjab (2.99mm), Florda Prince (2.89mm), Pratap (2.08mm) and Prabhat (1.69mm) by IBA-3000ppm. The highest callusing percentage (87.68%) was recorded in Early Grande followed by Shan-e-Punjab (84.51%), Florda Prince (78.67%), Pratap (60.54%), and Prabhat (57.31%) by treatment IBA-3000ppm. The highest number of roots/cutting (13.00) was recorded in Early Grande followed by Shan-e-Punjab (12.00), Florida Prince (10.60), Pratap (5.00) and Prabhat (4.50) by the treatment IBA-3000ppm. It is concluded that IBA at 3000 ppm was found to be the best treatment for propagation of peach through hardwood cuttings.