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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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Author: PRAJAPATI RONAKKUMAR RAMESHBHAI × End date: 2017 × Start date: 2017 × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    CONSORTIUM DEVELOPMENT FROM PHYLLOSPHERIC AND RHIZOSPHERIC METHYLOTROPHIC BACTERIA OF PADDY AS LIQUID PLANT PROBIOTICS AND ITS EFFICACY ON CV. GURJARI
    (AAU, Anand, 2017) PRAJAPATI RONAKKUMAR RAMESHBHAI; Dr. R. V. Vyas
    Phyllospheric methylotrophic bacteria were isolated from leaf surface of paddy from Anand, Thasra and Nawagam Tehshil on NMS medium supplemented with methanol (1%) by Leaf imprinting technique. Three native phyllospheric efficient methylotrophic bacterial isolates (M 3, M 10 and M 15) selected for detailed studies and found compatible with three rhizospheric methylotrophic bacteria (AAU reference strains) viz. Bacillus aerius AAU M 8, Panibacillus illinoisensis AAU M 17 and B. megaterium AAU M 29, and were used for consortium development to test its efficacy on paddy cv. Gurjari. Screening and characterization through 16S rRNA sequencing was carried out to identify three selected isolates. Beneficial native phyllospheric methylotrophic bacteria have additional ability to promote plant growth through one or more mechanisms (PGPR traits) viz. phosphorus and potash solubilization; ii production of phytohormones like indole-3-acetic acid (IAA) and siderophores production; tolerance to high pH and protection against pathogenic fungi as antagonist and possessing nifH gene for nitrogen fixation. Production of useful enzymes by isolates like methane degradation sMMO and MDH, as well as lipase and protease were confirmed in the laboratory. Plant growth promotion activity of phyllospheric methylotrophic bacterial isolates was studied in vitro through seed treatment of rice seeds were found to improve germination with better root and shoot. The experiment laid out in Randomized block design with four replication at Agricultural Research Station for irrigated crops, Thasra during 2014 and 2015. The treatments comprised of (T1: 100% RDF, T2: 80% RDF, T3: 60% RDF, T4: 80% RDF + consortium, T5: 60% RDF + consortium, T6: Only consortium and T7: Absolute control). The results indicated that T4 treatment showed significantly higher growth parameters viz., plant height, chlorophyll content, number of effective tiller/plant, root length, shoot fresh weight, shoot dry weight, yield attributing component viz. number of penicle/hill, length of penicle, number of grain/penicle, grain weight, grain yield, straw yield and higher methylobacterium count with better net return and saving of NPK chemical fertilizers (20%) followed by T1, T2 and T5 treatments over control.
  • Thumbnail Image
    ThesisItemOpen Access
    CONSORTIUM DEVELOPMENT FROM PHYLLOSPHERIC AND RHIZOSPHERIC METHYLOTROPHIC BACTERIA OF PADDY AS LIQUID PLANT PROBIOTICS AND ITS EFFICACY ON CV. GURJARI
    (AAU, Anand, 2017) PRAJAPATI RONAKKUMAR RAMESHBHAI; Dr. R. V. Vyas
    Phyllospheric methylotrophic bacteria were isolated from leaf surface of paddy from Anand, Thasra and Nawagam Tehshil on NMS medium supplemented with methanol (1%) by Leaf imprinting technique. Three native phyllospheric efficient methylotrophic bacterial isolates (M 3, M 10 and M 15) selected for detailed studies and found compatible with three rhizospheric methylotrophic bacteria (AAU reference strains) viz. Bacillus aerius AAU M 8, Panibacillus illinoisensis AAU M 17 and B. megaterium AAU M 29, and were used for consortium development to test its efficacy on paddy cv. Gurjari. Screening and characterization through 16S rRNA sequencing was carried out to identify three selected isolates. Beneficial native phyllospheric methylotrophic bacteria have additional ability to promote plant growth through one or more mechanisms (PGPR traits) viz. phosphorus and potash solubilization; ii production of phytohormones like indole-3-acetic acid (IAA) and siderophores production; tolerance to high pH and protection against pathogenic fungi as antagonist and possessing nifH gene for nitrogen fixation. Production of useful enzymes by isolates like methane degradation sMMO and MDH, as well as lipase and protease were confirmed in the laboratory. Plant growth promotion activity of phyllospheric methylotrophic bacterial isolates was studied in vitro through seed treatment of rice seeds were found to improve germination with better root and shoot. The experiment laid out in Randomized block design with four replication at Agricultural Research Station for irrigated crops, Thasra during 2014 and 2015. The treatments comprised of (T1: 100% RDF, T2: 80% RDF, T3: 60% RDF, T4: 80% RDF + consortium, T5: 60% RDF + consortium, T6: Only consortium and T7: Absolute control). The results indicated that T4 treatment showed significantly higher growth parameters viz., plant height, chlorophyll content, number of effective tiller/plant, root length, shoot fresh weight, shoot dry weight, yield attributing component viz. number of penicle/hill, length of penicle, number of grain/penicle, grain weight, grain yield, straw yield and higher methylobacterium count with better net return and saving of NPK chemical fertilizers (20%) followed by T1, T2 and T5 treatments over control.