Thumbnail Image

Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

Browse

20151
Reset filters
Subject: Veterinary Public Health × Author: PATEL, SWATI N. × End date: 2015 × Start date: 2015 ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    ISOLATION, IDENTIFICATION AND DETECTION OF VIRULENCE FACTOR ASSOCIATED GENES OF CAMPYLOBACTER SPP. FROM BUFFALO MEAT
    (AAU, Anand, 2015) PATEL, SWATI N.; BRAHMBHATT, M. N.
    Campylobacter spp. has been recognized as one of the most frequent cause of gastrointestinal illness in humans throughout world. In recent years, the frequency of human enteritis caused by Campylobacter spp. has exceeded those caused by salmonella especially in developed countries. The association of zoonotic Campylobacters with chronic and life threatening complications such as Guillain- Barre syndrome, reactive arthritis, haemolytic uraemic syndrome, abortion, meningitis etc. which increases the public health concern of this pathogen worldwide. Campylobacter is normal inhabitant of intestinal tract of food animals and faulty handling, lack of sanitation at processing and improper storage has been considered as the major factor contaminating all types of animal foods. Inadequately cooked meat, unpasteurized milk and contaminated drinking water are the most common sources for epidemic and sporadic food borne cases. Therefore, the present study was undertaken with the objective to isolate identify and characterize the Campylobacter spp. from raw buffalo meat intended for the human consumption available in the retail meat market in Anand, Gujarat. A total 200 samples including 75 beef, 75 liver, 25 intestinal content and 25 water samples were collected aseptically and processed immediately after arrival. The samples were enriched into 25 ml of Blood free Campylobacter broth base containing CCDA selective supplement. Mixed properly and incubated at under microaerophilic condition (85% N2, 5% O2 and 10% CO2) at 42° C for 48 hours and further inoculation on blood free Campylobacter selective agar (mCCDA). The presumptive Campylobacter isolates were further confirmed at genus and species level by the series of biochemical tests and also confirmed by polymerase chain reaction assay. The confirmed isolates were subjected for the antibiotic susceptibility test using seven antibiotics, detection of virulence determinants by PCR. In the present study, 7 out of 200 (3.5%)) samples in which 2 out of 75 (2.67 %)) buffalo beef, 3 out of 75 (4 %) liver, 2 out of 25 (8 %>) intestinal content and no any water sample were found positive for the Campylobacter spp. In all the seven isolates the only identified species was Campylobacter jejuni. All the seven isolates of Campylobacter were further processed to study antibiotic resistance and sensitivity pattern against seven antibiotics by using agar disc diffusion technique. All Campylobacter isolates were resistant to Ciprofloxacin (100%)), where as 85.71% and 28.57% resistant to Nalidixic acid and Tetracycline, respectively. All the Campylobacter isolates were sensitive to Chloramphenicol, Erythromycin, Gentamicin and Strepyomycin sulphate (100%)) followed by Tetracycline (71.42%o) and Nalidixic acid (14.28%). All the isolates were also subjected for in vitro detection of five virulence gene encoding for viz., Flagellin gene (flaA), Campylobacter adherence gene (CadF), Invasion associated marker (iamA) gene. Flagellar synthesis and modification (flgR) and Cytolethal distending toxin Subunit B gene (cdtB) by polymerase chain reaction (PCR) using specific primers. The result indicated that all the seven isolates were found positive for CadF, CdtB and JlgR gene (100%) while six isolates were positive for flaA gene (85.71%) and three isolates were positive for iamA gene (42.85%). Therefore, the presence of Campylobacter in buffalo meat confirms that raw meat consumption could be a significant risk factor for human beings and there is need for routine study of this pathogen in buffalo meat to decrease the chances of infection with Campylobacter by consumption.