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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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Subject: Veterinary Public Health × Author: DHANDE, MANOJ S × End date: 2019 × Start date: 2010 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    ISOLATION, IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF AEROMONAS FROM MARKET MILK
    (AAU, Anand, 2010) DHANDE, MANOJ S; Brahmbhatt, M. N.
    The aim of the present study was to isolate, identify and characterize Aeromonas species from raw milk in an around Anand market. A total of 160 samples were processed for estimating prevalence o^ Aeromonas spp. Out of those 160 samples tested, 29 Aeromonas isolates were recovered. On the basis of biochemical characterization 19 (13.6%) isolates of ^. sobria, 6 (4.3%) isolates of A. hydrophila and 4 (2.8%) isolates oiA. caviae were detected in raw milk. When different selective culture media were evaluated for isolation of Aeromonas spp. from raw milk it was found that percent recovery of Aeromonas isolates were more from Ampicillin Dextrin Agar (82.75%) as compared to Aeromonas Starch DNAse agar (62.06%)) All 29 Aeromonas isolates were tested for presence of aerolysin, haemolysin and enterotoxin gene. None of the isolate showed presence of aerolysin and enterotoxin, while overall prevalence of haemolysin gene was 78.78 per cent. In A. hydrophila, 54.54 per cent; A. caviae, 37.5 per cent and in A. sobria, 91.48 per cent isolates were found to possess haemolysin gene. All the isolates of Aeromonas were subjected to antimicrobial dmg sensitivity test against gentamicin, chloramphenicol, ciprofloxacin, kanamycin, bacitracin, rifampicin, tetracycline, ampicillin, penicillin G and erythromycin. Maximum sensitivity pattern was recorded with chloramphenicol (86.20 per cent), bacitracin (31.03 per cent) gentamicin and rifampicin (20.68 per cent) each, erythromycin (17.24 per cent), kanamycin (6.89 per cent). The resistance pattern of Aeromonas isolated from raw milk to various antibiotics was observed as cent per cent resistance towards tetracycline, ampicillin, penicillin G, ciprofloxacin (93.10 per cent), kanamycin (72.41 per cent), bacitracin (68.96 per cent) followed by gentamicin (65.51 per cent), rifampicin (62.06 per cent), erythromycin (58.62 per cent. Owing to the potential hazard of pathogenic Aeromonas spp., it was concluded that it is necessary to put more emphasis on food hygiene. Therefore, the surveillance of potential contaminant is crucial for sustenance of public health.