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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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20151
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Subject: Veterinary Pathology × Author: ACHARYA, ASHOK T. × Start date: 1980 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    COMPARATIVE STUDY ON CYTOLOGY AND HISTOPATHOLOGY FOR DIAGNOSIS OF CANINE NEOPLASMS
    (AAU, Anand, 2015) ACHARYA, ASHOK T.; Ghodasara, D. J.
    The present research work was carried out to know agreement between cytological and histopathologcal diagnosis of canine neoplasms. Diagnostic accuracy, sensitivity and specificity of fine needle aspiration cytology (FNAC) were determined, using histopathology as a reference method. Research work was carried out at Veterinary college, Anand, in the Department of Veterinary Pathology. Study materials were collected from different breeds of dog received at Department Veterinary Surgery and Radiology, Veterinary college, Anand. The objectives of the current research were to study the gross morphology of tumour masses, cytological and histopathological diagnosis of tumours, and comparison between these two methods. The present study comprised of 40 cases of canine spontaneously occurring tumours. Gross morphologic study included size, shape, weight, consistency, colour of cut surface, and location of all tumour masses. Pre-operatively, aspirates were collected from all tumour masses using 22 G needles and 5 ml syringes to evaluate cytological diagnosis of tumours. 95% ethanol was used as a fixative. All the cases were proceeded for cytological diagnosis by employing FNAC technique and stains employed were Papanicolaou, MGG, H and E, Giemsa and Wright's. Tissue materials were collected for histopathological examination. Labrador was the most commonly affected breed Females showed more cases as compared to male dogs. In most of cases 5 to 12 years old animals were affected. Cytologically, tumours were categorized as benign or malignant based on general and nuclear criteria. Tumours were classified as epithelial, mesenchymal, round cell tumours. Cytologically, 11 cases were diagnosed as malignant and 29 were reported as benign. Cytologically, 21 declared as epithelial, 14 as round cell and 5 as mesenchymal tumours. Epithelial tumours included mammary gland tumours (15), perianal gland adenoma (1), basal cell carcinoma (1), squamous cell carcinoma (2), seminoma (1), and melanoma (1). Round cell tumours were TVT (11) and mast cell tumour (mastocytoma) (3), while mesenchymal tumours were fibrosarcoma (1) and lipoma (4). Exfoliation was higher in epithelial and round cell tumours than mesenchymal tumours. Cytological features of malignant tumours were presence of large numbers of neoplastic cells, anisocytosis, anisokaryosis, presence of mitotic figures, nuclear abnormalities (like double nucleus, nuclear molding), nucleolar abnormalities (such as multiple nucleoli, anisonucleosis), abnormal chromatin and increased nuclear: cytoplasmic ratio. Benign tumours did not show such features although in certain tumours nuclear and nucleolar abnormality, mitotic figures and abnormal chromatin were found. In present study, May-Grunwald- Giemsa (MGG) and Hematoxylene and eosin stain proved best staining ability with best cellular details obtained when stained with these two methods. All tissue sections were stained with H and E method, but for confirmation of certain tumours, like mastocytoma (toluidine blue) and fibrosarcoma, (masson's trichrome) special stains were used. All tumours were microscopically examined and definitive diagnosis was made. Histopathologically, 11 tumours were diagnosed as malignant while 29 were diagnosed as benign. Histopathologically also 21 tumours were diagnosed epithelial origin, 14 round cell, and 5 as mesenchymal. Among 15 mammary tumours, 10 cases were included in benign, while remaining 5 were included to malignant category. All TVTs, mastocytomas and lipomas were diagnosed as benign. Finally, comparison between cytological and histopathological diagnosis was made, as latter was kept reference method. In 95% (38/40) cases, correct correlation was recorded between cytological and histopathological diagnosis. FNAC showed 90.90% sensitivity and 96.55% specificity for diagnosis canine neoplasms. It may be concluded from present study that cancer is the most important cause Of death in canines. Proper early diagnosis of canine neoplasms helps the clinicians to recognize them in time, to provide a correct prognosis and a therapy as effective as possible. As per present cytological investigation, the diagnostic accuracy for differentiation between malignant and benign canine neoplasms was very high (95%). This report may open the way up for using FNAC much more as a reliable and useful diagnostic procedure for evaluation of neoplasms in canines. However, proper technique and ample exercise are of vital importance for high success rate in cytological diagnosis of canine neoplasms.