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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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20151
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Subject: Veterinary Pathology × Author: ABHILASH, T. × Start date: 1980 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    PATHOLOGICAL STUDIES ON SUSPECTED INFECTIOUS BRONCHITIS-QX STRAIN INFECTION IN LAYER BIRDS
    (AAU, Anand, 2015) ABHILASH, T.; JOSHI, B. P.
    The present research work was carried out to know the pathological alterations caused by Infectious Bronchitis virus in a commercial layer flock as well as to ascertain the strain of the virus involved in the infection. The pathological study comprised the information in relation to production drop, mortality, duration of mortality, clinical signs, gross and histopathological examination of visceral organs, serological estimation of antibody titer, isolation of virus by egg inoculation and its further confirmation and characterization by RT-PCR and subsequent gene sequencing. During the study mortality was observed in the affected flock in an even manner ranging from 0.48 to 0.78 percent throughout the period of 26-54 weeks and the total mortality observed was 15.3% during the period. From 20th week onwards th the flock showed a drop in egg production and at the 26th week peak production reached up to 89.5% followed by a decline. The egg production drop up to 54 weeks of age ranged between 1.9 to 21.3% with an average production drop of 15.1 percent. The affected birds showed typical clinical manifestation of penguin gait appearance with pendulous abdomen from 32 weeks onwards. The eggs laid by the birds were misshapened or soft shelled with watery albumin. Gross lesions were characterized by cystic oviducts with accumulation of large quantities of clear water like fluid in majority of the affected birds. In a comparatively less number of cases there observed accumulation of abnormally formed eggs or large masses of fibrinous yolk material in the oviduct. In certain instances internal layers and small cystic right oviducts were observed. No significant gross morphological lesions were noticed in organs other than oviduct. The microscopic lesions were mainly observed in the oviduct, trachea and kidney. They were of the nature of severe decrease to complete absence of glandular tissue, atrophy of the villi with loss of mucosal epithelium and infiltration of mononuclear cells and plasma cells in the lamina propria of the affected oviduct; deciliation and desquamation of lining epithelium, focal areas of congestion, mononuclear inflammatory cells infiltration in lamina propria of trachea; and mild to moderate interstitial nephritis with intertubular haemorrhages in the kidney parenchyma. The inoculation of supernatant obtained after trituration and centrifiigation of pooled tissue samples (oviduct, trachea, kidney and caecal tonsils) in 9-11 day old SPF embryonating eggs resulted in death of all the embryos following incubation up to 7 days post inoculation after the third passage. The typical dwarfing and curling showed by all the dead embryos confirmed the presence of the IB virus. Diagnostic RT-PCR with SI gene region was successful for targeted amplification and detection of IBV. Sequence analysis showed that the isolate was like 4/91 strain of IBV. Based on major presenting clinical signs of penguin gait appearance and gross pathological lesions of cystic dilatation of oviduct in the affected layer flock and similar earlier reports of IB-QX infection in layer birds having same clinical signs and gross lesions in other countries, the infection was suspected as IB-QX. But it turned out to be 4/91 strain of IBV instead of QX strain on viral gene sequencing. In India this is the first study concluding the involvement of IBV 4/91 strain in inducing reproductive tract damages particularly cystic oviducts in commercial layer birds leading to production drop.