Thumbnail Image

Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

Browse

20131
Reset filters
Subject: Veterinary Parasitology × Author: CHOURASIA, REETIKA × End date: 2013 × Start date: 2013 × Type: Thesis × Thesis Type: M.V.Sc. ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    Transcriptome Analysis of Paramphistomum cervi of water buffalo (Bubalus bubalis) using next generation sequencing
    (AAU, Anand, 2013) CHOURASIA, REETIKA; PATEL, P. V.
    Rumen flukes are economically important parasites (Platyhelminthes: Trematoda: Digenea) that attack livestock adversely thereby affecting their productivity. In spite of its economic importance, molecular biology of the Paramphistomum cervi and its interaction with its hosts is still unknown. Advances in transcriptomic and bioinformatics provide biologically relevant insights into parasites, their developmental stages and their relationships with their hosts at the molecular level. The present study elucidates the first transcriptome and gene expression profiling of the adult stage of Paramphistomum cervi using next-generation (high throughput) sequencing and advanced in silico analyses. Expression level for predicted proteins of Paramphistomum cervi of buffalo were determined and classified based on homology, gene ontology and pathway mapping. These findings are expected to provide new insights into the genetic architecture and pathophysiology of Paramphistomum cervi and for the development of improved interventions for disease control. It will also facilitate a more fundamental understanding of Paramphistomes biology, evolution and the host-parasite interplay. Moiphological characteristics of adult fluke were identified as conical shape, elongate, curved ventrally, with evenly curved dorsal and ventral borders. Cuticle is provided with prominent tubercules/papillae on anterior l/3rd to half of the body. Tubercles are more extensive ventrally. Acetabulum is subtemiinal. hitestinal caeca have 7 nearly identical bends with ventrally directed temiinal part. Testes are tandem, oval or angularly oval or spherical and are deeply lobed. Gross examination of affected rumen showed, anaemic rumen with atrophied, degenerated and sloughing tips of villi. Removal of flukes revealed marked knobs at the attachment sites. Histopathology of rumen revealed proliferation of epithelium in the vicinity of flukes, along with villous atrophy and infiltration of macrophages and eosinophils. Transcriptome analysis of adult stage of Paramphistomum ceni was carried out at Department of Animal Biotechnology. Total RNA was extracted from parasites using TRIzol® (Invitrogen, UK)/ RNeasy® mini kit and mRNA isolation from the total RNA was carried out by using mRNA isolation kit. The quality and quantity of RNA and mRNA checked by running the sample on NanoDrop ND-1000 spectrophotometer. Concentration of RNA of adult fluke was 2,608 ng/µl and mRNA was 100 ng/µl. The cDNA library was constructed using the Ion Total RNA-Seq Kit v2. According to Qubit®Fluorometer, concentration of cDNA was 1.19 ng/µl and based on Aligent 2100 Bioanalyzer concentration of cDNA is 1.25 ng/µl.