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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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2010 - 201925
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Subject: Veterinary Microbiology × End date: 2019 × Start date: 2010 × Type: Thesis × Thesis Type: M.V.Sc. ×
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Now showing 1 - 9 of 25
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    ThesisItemOpen Access
    ISOLATION, ELECTROPHEROTYPING AND MOLECULAR CHARACTERIZATION OF BOVINE ROTAVIRUS FROM DIARRHOEAL SAMPLES OF BOVINE CALVES
    (Department of Veterinary Microbiology College of Veterinary Science and Animal Husbandry Anand Agricultural University Anand, 2019) Golaviya Akash V.; Dr. R. A. Mathakiya
    Among the infectious diseases of calves, acute gastroenteritis and neonatal calf diarrhoea are two major causes of mortality in neonates of farm animals caused by multiple etiological agents all over the world. Rotaviruses constitute the single major entity of potentially pathogenic gastroenteritis in animals and humans. Rotavirus is classified as a member of family Reoviridae, genus Rotavirus, with a distinct “cartwheel” structure when viewed in negative electron microscopy. Due to segmented nature of the Rotavirus RNA genome and wide host range, vast genetic and antigenic diversity exists amongst different isolates of Rotavirus. Bovine Rotavirus cause significant economic loss in the dairy and meat industry due to increased morbidity and mortality, treatment costs, and reduced growth rates. In neonatal calves, about 5-20% mortality rate is observed due to Bovine Rotavirus in calves. In India, the prevalence of Rotavirus in calf diarrhoea, below one month of age, ranges between 10-52%.
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    ThesisItemOpen Access
    IN VITRO EFFICACY OF STAPHYLOCOCCUS SPP. AND ESCHERICHIA COLI-SPECIFIC BACTERIOPHAGE AND DETECTION OF ANTIMICROBIAL RESISTANCE GENES IN PHAGE GENOME
    (DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2019) KULKARNI PRATIK MANOHAR; Dr. B. B. Bhanderi
    Antimicrobial resistance (AMR) has been a growing threat to the effective treatment of an ever-increasing range of infections caused by bacteria, fungi, viruses and parasites. The current lack of new antimicrobials on the horizon to replace those that become ineffective brings added urgency to the need to protect the efficacy of existing drugs, as well as to explore novel strategies for ameliorating the current situation with regards to AMR. AMR has also been observed among Staphylococcus spp., a major etiological agent for diseases such as mastitis in bovines, bumblefoot in poultry and pyoderma in dogs, and Escherichia coli, a major etiological agent for diseases such as colibacillosis in poultry, coliform mastitis in bovines, neonatal diarrhoea and joint ill in calves. Bacteriophages (phages) are viruses capable of infection and replication in bacterial cells and the potential of phages as a therapeutic tool has been known since phages have been known to exist.
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    ThesisItemOpen Access
    DETERMINATION OF ANTIBIOTIC SUSCEPTIBILITY BY PHENOTYPIC AS WELL AS GENOTYPIC METHODS AND MOLECULAR CHARACTERIZATION OF AVIAN PATHOGENIC ESCHERICHIA COLI
    (DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2018) Bhargav B. Limbachiya; Dr. R. A. Mathakiya
    The poultry production chain has constant advances in intensive management of animals and operations technology in genetics, nutrition and animal health. Higher quality products and food security ensure the credibility. However, the intensification of the production may cause increase health problems. Among various diseases, colibacillosis is one of the most important disease, which causes heavy economic losses in broilers as well as in layers. It is caused by pathotype APEC belongs to the group ExPEC
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    ThesisItemOpen Access
    PRE AND POST THERAPY MICROBIOLOGICAL AND MOLECULAR DETECTION AND QUANTITATION OF BACTERIA CAUSING CLINICAL MASTITIS IN COWS
    (DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2018) Vatalia Dip J.; Dr. B. B. Bhanderi
    Mastitis is one of the costliest diseases of dairy cattle. Mastitis is classified as subclinical and clinical mastitis. Clinical mastitis is a condition, which is characterized by local symptoms like swelling of the udder, heat and pain or systemic symptoms like fever, anorexia, depression with milk abnormalities like milk clots, flakes, watery secretions, blood in milk. About 150 species of microorganisms have been incriminated as the causal agents of mastitis. Some times in clinical mastitis after treatment there are reoccurrence of clinical mastitis. Looking to the economic important the present study was undertaken with the following objectives viz. cultural isolation, biochemical characterization and PCR based identification of bacterial pathogens, extraction of DNA from clinical mastitic milk and PCR based identification of major bacterial pathogens, post therapy isolation, identification and quantitation of bacteria by Real-Time PCR and antimicrobial susceptibility testing of the bacterial isolates
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    ThesisItemOpen Access
    DETECTION OF ESCHERICHIA COLI AND ROTAVIRUS FROM FECAL SAMPLES OF DIARRHEIC CALVES OF CATTLE AND BUFFALOES
    (DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2018) PATEL JAYESHKUMAR VIRABHAI; R. A. Mathakiya
    Among the infectious diseases of calves, neonatal diarrhoea is a matter of major concern and is caused by multiple etiological agents. Neonatal calf diarrhea (NCD) is a multifactorial complex syndrome including infectious as well as non-infectious factors related to the animal viz. immunological and nutritional status, the environment or the management Neonatal calves are at the greatest risk of diarrhea in a first month of their life. Infectious diarrhea is the most significant cause of morbidity and mortality in neonatal dairy calves throughout the world and it can be caused by many pathogens including Enterotoxigenic Escherichia coli (ETEC) and Rotavirus.
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    ThesisItemOpen Access
    DETECTION OF GENES FOR VIRULENCE ASSOCIATED FACTORS, ANTIBIOTIC RESISTANCE AND PLASMID PROFILE AMONG PASTEURELLA MULTOCIDA ISOLATES OBTAINED FROM ANIMALS AND AVIAN SPECIES IN GUJARAT
    (DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Parmar Rahul A.; Dr. B. B. Bhanderi
    The present study was undertaken with a view of isolation and identification of P. multocida from suspected animal and avian species of Gujarat. These isolates were studied for their biochemical behavior, in vitro antibiotic sensitivity pattern, P. multocida species specific PCR (PM-PCR), molecular characterization by PCR assay for capsular typing, detection of various virulence associated genes by PCR, detection of antibiotic resistance gene and plasmid profile.
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    ThesisItemOpen Access
    SERODETECTION OF Brucella ANTIBODIES AND CHARACTERIZATION OF Brucella ISOLATES FROM REPRODUCTIVE DISORDERS OF CATTLE AND BUFFALOES IN AND AROUND ANAND
    (Department of Veterinary Microbiology College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand, 2016) Ritesh N. Patel; Dr. Ashish Roy
    Brucellosis is a zoonotic infection caused by gram negative, facultative intracellular bacteria Brucella that are pathogenic for a wide range of animals and human beings. The disease has a considerable impact on human and animal health, as well as socioeconomic impacts, especially in which rural income relies largely on livestock breeding and dairy products. Brucella organisms are coccobacilli or short rods, arranged singly and less frequently in pairs or small groups. The genus Brucella belong to the α- proteobacteria group and consists of six recognized species: Brucella abortus, Brucella melitensis, Brucella suis, Brucella ovis, Brucella canis, Brucella neotomae and the strains recently discovered in marine mammals and in common vole (Microtus arvalis) and published under the respective species names of Brucella ceti, Brucella pinnipedialis and Brucella microti. Diagnosis is usually based on serological tests and/or cultivation. Serological evidence suggested that brucellosis is highly endemic in many parts of India.
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    ThesisItemOpen Access
    DETECTION OF PASTEURELLA MULTOCIDA LOAD IN EXPERIMENTALLY INFECTED MICE AND ITS DETECTION BY DIRECT BLOOD AND TISSUE POLYMERASE CHAIN REACTION
    (DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Patel Brijeshkumar Pravinbhai; Dr. B. B. Bhanderi
    The present study was undertaken to ascertain enumeration of P. multocida from blood and tissue at different time interval to experimental inoculation in mice and to confirm by cultural, biochemical, P. multocida PCR (PM-PCR), whole blood PCR and Tissue PCR. The blood and tissue was stored on FTA card at different temperature and PM-PCR was carried out at different time interval.
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    ThesisItemOpen Access
    SERODETECTION OF Brucella ANTIBODIES AND CHARACTERIZATION OF Brucella ISOLATES FROM REPRODUCTIVE DISORDERS OF CATTLE AND BUFFALOES IN AND AROUND ANAND
    (AAU, Anand, 2016) PATEL, RITESHKUMAR NARSINHBHAI; Roy, Ashish
    Brucellosis is a zoonotic infection caused by gram negative, facultative intracellular bacteria Brucella that are pathogenic for a wide range of animals and human beings. The disease has a considerable impact on human and animal health, as well as socioeconomic impacts, especially in which rural income relies largely on livestock breeding and dairy products. Brucella organisms are coccobacilli or short rods, arranged singly and less frequently in pairs or small groups. The genus Brucella belong to the aproteobacteria group and consists of six recognized species: Brucella abortus, Brucella melitensis, Brucella suis. Brucella ovis. Brucella canis, Brucella neotomae and the strains recently discovered in marine mammals and in common vole (Microtus arvalis) and published under the respective species names oi Brucella ceti, Brucella pinnipedialis and Brucella microti. Diagnosis is usually based on serological tests and/or cultivation. Serological evidence suggested that brucellosis is highly endemic in many parts of India. The present study was undertaken to detect Brucella antibodies in serum, isolation of Brucella organisms from various reproductive disorders (abortion, R.O.P., endometritis, infertility and repeat breeder) and PCR based identification and characterization of Brucella isolates. For detection of Brucella antibodies in serum, two serological methods viz., i-ELISA and RBPT were carried out. For isolation of Brucella organisms Brucella agar medium (BAM) was used and for detection oi Brucella DNA by PCR three different genus specific primer pairs viz., B4/B5, JPF/JPR and F4/R2 were used. Species identification of various brucella isolates obtained from cattle and buffalo were carried out by AMOS PCR and Bruce-ladder PCR. During the study period, total 434 sera samples comprising of 361 from cattle and 73 from buffaloes were screened using i-ELISA, of which 51 (14.12%) and 22 (30.13%) were found positive, respectively. The overall seropositive sera among 434 sera samples was found to be 73 (16.80%). In present study, seroprevalence of Brucella antibodies from gynaecological disorder cases was studied from the 434 serum samples comprises of 361 from cattle and 73 buffaloes by RBPT. Forty eight sera samples were found to be positive by RBPT indicating seropositivity of 11.05%. Among the two serological tests employed for detection of Brucella antibody in serum of cattle and buffaloes, the highest positive results were obtained by ELISA (16.80%), followed by RBPT (11.05%). Prevalence of Brucella antibody in abortion and R.O.P. were studied using i- ELISA and RBPT, recorded 34.69% and 22.44% positivity in cattle and buffaloes, respectively whereas from reproductive disorders (endometritis, infertility and repeat breeder) 11.69% by i-ELISA and 7.25% by RBPT were found to be overall seropositive in cattle and buffaloes. Attempt for cultural isolation was done by inoculating 114 various samples like vaginal swabs, aborted material, milk and placenta. Of these, only 3 samples yielded Brucella organisms on Brucella agar medium. The isolates were identified by cultural, morphological, biochemical characteristics test and further confirmed by PCR using different genus and species specific primer pairs. Colony PCR was carried out using various genus specific primer pairs for the confirmation oi Brucella isolates for different gene. The desired product of 223 bp using B4/B5 (BCSP31 gene) primer pair was amplified in all the 3 isolate (including reference strain). However, desired product of 193 bp using JPF/JPR (omp2 gene) primer pairs was amplified except isolates C2 (cow). Whereas product of 905 bp using F4/R2 (16S rRNA gene) primer pairs was amplified in all the isolates. Furthermore for the species identification, multiplex PCR assays were employed that were AMOS PCR and Bruce-ladder PCR. AMOS PCR could amplify B. abortus specific primer, whereas Bruce-ladder also could able to identify all 3 isolates as B. abortus. All the isolates of Brucella abortus from cows (C1, C2 and C3) were found to be 100% sensitive to Streptomycin, Tetracycline, Amikacin, Erythromycin, Pefloxacin, Amoxyclav, Spectinomycin and Norfloxacin whereas all the three B. abortus isolates were found to be resistant to Ampicillin.