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Anand Agricultural University, Anand
Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur
AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.
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ThesisItem Open Access DETECTION OF GENES FOR VIRULENCE ASSOCIATED FACTORS, ANTIBIOTIC RESISTANCE AND PLASMID PROFILE AMONG PASTEURELLA MULTOCIDA ISOLATES OBTAINED FROM ANIMALS AND AVIAN SPECIES IN GUJARAT(DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Parmar Rahul A.; Dr. B. B. BhanderiThe present study was undertaken with a view of isolation and identification of P. multocida from suspected animal and avian species of Gujarat. These isolates were studied for their biochemical behavior, in vitro antibiotic sensitivity pattern, P. multocida species specific PCR (PM-PCR), molecular characterization by PCR assay for capsular typing, detection of various virulence associated genes by PCR, detection of antibiotic resistance gene and plasmid profile.ThesisItem Open Access SERODETECTION OF Brucella ANTIBODIES AND CHARACTERIZATION OF Brucella ISOLATES FROM REPRODUCTIVE DISORDERS OF CATTLE AND BUFFALOES IN AND AROUND ANAND(Department of Veterinary Microbiology College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand, 2016) Ritesh N. Patel; Dr. Ashish RoyBrucellosis is a zoonotic infection caused by gram negative, facultative intracellular bacteria Brucella that are pathogenic for a wide range of animals and human beings. The disease has a considerable impact on human and animal health, as well as socioeconomic impacts, especially in which rural income relies largely on livestock breeding and dairy products. Brucella organisms are coccobacilli or short rods, arranged singly and less frequently in pairs or small groups. The genus Brucella belong to the α- proteobacteria group and consists of six recognized species: Brucella abortus, Brucella melitensis, Brucella suis, Brucella ovis, Brucella canis, Brucella neotomae and the strains recently discovered in marine mammals and in common vole (Microtus arvalis) and published under the respective species names of Brucella ceti, Brucella pinnipedialis and Brucella microti. Diagnosis is usually based on serological tests and/or cultivation. Serological evidence suggested that brucellosis is highly endemic in many parts of India.ThesisItem Open Access DETECTION OF PASTEURELLA MULTOCIDA LOAD IN EXPERIMENTALLY INFECTED MICE AND ITS DETECTION BY DIRECT BLOOD AND TISSUE POLYMERASE CHAIN REACTION(DEPARTMENT OF VETERINARY MICROBIOLOGY COLLEGE OF VETERINARY SCIENCE AND ANIMAL HUSBANDRY ANAND AGRICULTURAL UNIVERSITY ANAND, 2017) Patel Brijeshkumar Pravinbhai; Dr. B. B. BhanderiThe present study was undertaken to ascertain enumeration of P. multocida from blood and tissue at different time interval to experimental inoculation in mice and to confirm by cultural, biochemical, P. multocida PCR (PM-PCR), whole blood PCR and Tissue PCR. The blood and tissue was stored on FTA card at different temperature and PM-PCR was carried out at different time interval.ThesisItem Open Access STUDIES ON IMMUNOMODULATION BY LEVAMISOLE ALONG WITH VACCINATION IN CHICKS AGAINST RANIKHET DISEASE AND IN CALVES AGAINST HAEMORRHAGIC SEPTICAEMIA(AAU, Anand, 1985) VYAS, GIRISH P.; DHOLAKIA, P. M.The present study was aimed assessment of the immunomodulatory effect of levamisole along with vaccination in chicks against RanikhetDisease (R.D.) and in calves against Haemorrhagic Septicaemia (H.S.) in relation to serum antibody titres, effect of dose of levamisole, total immunity period and electrophoretic pattern of serum proteins after immunization and treatment with levamisole. In all 560 serum samples from 140 chicks belonging to Central Poultry Research Station and 100 serum samples from 20 calves belonging to Livestock Research Station of Gujarat Agricultural University, Anand Campus, Anand were subjected to Haemaggulutination Inhibition (H.I.) test, Passive Haemagglutination (PHA) test, Sodium sulfite precipitation test and Agar gel electrophoresis.ThesisItem Open Access STUDIES ON MYCOTIC MASTITIS IN EXOTIC AND CROSSBRED CATTLE WITH SPECIAL REFERENCE TO ITS INCIDENCE AND DIAGNOSIS(AAU, Anand, 1984) SIMARIA, MULJI B.; DHOLAKIA, P. M.The present study was taken up to assess the incidence of mycotic infections of under in apparently healthy quarters of lactating cows and diagnosis in milk samples from clinical cases, Simultaneously, the prevalence of subclinical mastitis (SCM), and pathogenicity of fungal strains were also studied. The milk samples from 150 lactating cows (53 Jersey, 30 Kankrej and 67 crossbred) were collected. In addition to this 82 milk samples from clinical cases of mastitis received in Department of Bacteriology were also included in the present study. Subclinical mastitis (SCM) was recorded as 15.33 and 5.27 per cent on animal and quarter basis with California Mastitis Test. Although, incidence of SCM was highest in Jersey herd (8.82% quarterwise), fungal isolation was as low as 2.45 per cent, Hence CMT did not prove efficient enough to detect fungal infection in apparently normal udders.ThesisItem Open Access STUDIES ON ADVANCED METHODS OF ASCERTAINING BRUCELLOSIS IN CATTLE(AAU, Anand, 1985) PATEL, J. B.; JHALA, V. M.The disease Brucellosis caused by the infection of Brucella abortus in cattle adversely affects the economy of livestock rearing due to losses of calves created by abortions in suffering animals along with loss in milk production. The disease is worldwide in distribution and calves lost due to abortions create highly adverse economic impact on the animal husbandry development. The disease also occupies a special importance being zoonotic in nature. It is# therefore, essential to diagnose the disease in a herd as early as possible for its effective control. It is now well established that the Brucella organisms being the facultative Intracellular organisms the cellular immunity plays a major role in the host resistance. In view of the above facts, the present work was carried out in cows at Livestock Research Station, Gujarat Agricultural University, Sardar Krushinagar, employing following six parametars to study their efficacy. (1) Screening of individual cow by milk ring test using ABR i.e. Aboirtus Bang Ring Antigen. (2) Above cows and the cows which were not in milk but having the history of abortion, were subjected to serum plate agglutination test. (3) All above sera samples were further examined by Serum Tube Agglutination Test (STAT). (4) Fourteen sera samples from positive, doubtful and negative reactors to STAT were sent to International Brucella Laboratory, Indian Veterinary Research Institute, Izatnagar for confirmation by complement fixation test. (5) Determination of conglutinin (K) and immunoconglutinin (IK) level in sera of positive, doubtful and negative reactors to STAT . (6) Measurement of cell mediated immunity in vitro by leucocyte migration inhibition test. Prom the results obtained it could be concluded that:- i)The serological tests namely milk ring test plate agglutination test, tube agglutination test may serve as useful tool for diagnosis of brucellosis in a herd. ii) The level of K and IK in sera of positive, doubtful or negative reactors to STAT have no significant difference and there was no significant correlation with agglutination titre, hence the determination of K and IK may not be of use in diagnosis of Brucellosis. iii) The Brucella organisms are being the facultative intracellular micro organisms, the cell mediated immunity plays an important role in resistance. The measurement of cell mediated immunity in vitro by leucocyte migration inhibition test led to the conclusion that this test may be of very great value as a modern advanced method for the diagnosis of Brucellosis where cellular immunity is considered to play a significant role.ThesisItem Open Access STUDIES ON MICROFLORA OF BOVINE SEMEN AND THEIR EFFECT ON SEMEN QUALITY(AAU, Anand, 1983) KHER, HIRABHAI N.; DHOLAKIA, P. M.Tho present study ims alaed to assess the baoterial load and types of organisms presant In saaien and their relation to semen quality. The study also included antibiotie sensitivity pattern of the isolates in vitro. Totally 45 bulls belonging to (a) Regional A. I. Centre, Rajkot, (b) Central Semen Collection Station, Mehsana, and (c) A. I. Centre, Godhra were studied during the year 1982-83. Bacterial load in the range frota 630 to 14375 organisms per ml for neat semen and in the range from 12300 to 130000 per ml for preputial washings had been encountered. The bacterial load from neat semen was high in summer season followed by monsoon and winter season, while that of preputial washings was high in monsoon season followed by summer and winter season. The semen quality was found fairly good , irrespective of bacterial load of ejaculates. Within the centre, bacterial load of neat semen and preputial washings was higher in buffalo bulls than that of cow bulls and was not varying according to type of sheath.ThesisItem Open Access ASSESSMENT OF NOVEL N-GENE TARGET FOR DETECTION OF PESTE DES PETITS RUMINANTS (PPR) VIRUS BY RT-PCR AND CHARACTERIZATION BY RFLP PROFILE(AAU, Anand, 2006) CHANDRA, VARTIKA; Jhala, M. K.Peste des Petits Ruminants (PPR) is an economically important viral disease of sheep and goats, characterized by high fever, ocular and nasal discharge, pneumonia, necrosis and ulceration of the mucous membrane and inflammation of gastrointestinal tract leading to severe diarrhea. The disease is caused by a Morbillivirus of the family Paramyxoviridae. The disease is highly contagious causing varying degree of morbidity and mortality in susceptible animals. In India, severity of the disease is more pronounced in goats than in sheep and with a combined susceptible population of about 200 million, it is one of the major threats to the small ruminant population of the country. The present study was aimed to study the incidence of Peste des petits ruminants virus (PPRV) in goats of selected areas of Gujarat by sandwich ELISA and to derive estimates of overall, locationwise, agewise and sexwise incidence and samplewise positivity rates. In addition, the study also involved standardization and application of novel Nucleocapsid (N) gene based RT-PCR, for diagnosis of PPR. Further, an attempt was also made to access possible genetic variation among the field and vaccine PPRVs by RFLP profile. A total of 46 clinical samples from 31 goats suspected of PPRV from selected areas of Gujarat were tested by sandwich-ELISA, of which 23 animals were found positive yielding an overall incidence rate of 74.19 percent. Out of 18 animals suspected of PPR at Clinical Service Complex, Veterinary College. Anand, 15 (83.33%) were found positive; whereas four (80%) out of five samples from Koth, two (66.67%)) out of three samples from Vasna and two (40%) out of five samples from Harsoli yielded positive results. Out of the 31 animals, 13, 11, 6 and one animals belonged to age groups of 0-12, 12-24, 24-48 and >48 months, respectively, showing the respective incidence of PPRV as 84.61, 63.63, 66.67 and 100 per cent. Incidence rate of PPR by sandwich-ELISA was 80%) and 71.43%) in males and females, respectively. The PPRV antigen could be detected slightly more in blood samples (73.33%) than in nasal swabs (66.67%o), in case where both the sample types were taken from the same animals. All the 46 clinical samples, a reference vaccine virus (Sungri isolate) and a blood sample from apparently healthy goat were processed for RNA extraction using TRI Reagent . RNA samples showing acceptable purity and concentration were reverse transcribed with random hexamers to generate cDNA templates, which were subjected to PCR amplification using in silico designed N-gene specific primer pair N1-N2. Reference vaccine virus as well as fifteen (32.61%) of the 46 clinical samples, including fourteen blood samples and one nasal swab, produced the desired amplion of approximately 463 bp with primer pair N1-N2. The amplicons from all the positive samples and the vaccine virus migrated similarly in the gel. Thirty one (67.39%)) samples including 12 blood samples, 17 nasal swabs and two tissue samples as well as the blood sample taken from apparently healthy goat failed to produce the targeted amplification with the primer pair N1-N2. Out of 46 clinical samples tested for PPRV, 31 and 15 samples were positive by sandwich-ELlSA and RT-PCR, respectively. "None of the samples negative in sandwich- ELISA yielded positive amplification in RT-PCR. Relative to sandwich-ELlSA, sensitivity and specificity of the RT-PCR was 48.39 and 100.00 per cent, respectively. Overall agreement between the two tests was 65.22 per cent. RT-PCR products (463 bp) amplified with primer pair N1-N2, were further digested with two restriction enzymes viz. Pst-I and Alu-I for RFLP analysis. RT-PCR products of the representative samples from each of the three locations and the reference vaccine virus produced similar RFLP patterns with each of the two REs, indicating absence of genetic variation at the corresponding restriction sites of these two REs. Digestion of RT-PCR products with Alu-l yielded a fragment of 395 bp, whereas the other fragment was not visible. Similarly, digestion with Pst-I yielded a single fragment of405bp.ThesisItem Open Access SEROEPIDEMIOLOGY AND ELECTROPHEROGRAM STUDIES OF BLUETONGUE VIRUS(AAU, Anand, 2000) Bhalodiya, M. B.; Jhala, M. K.Bluetongue (BT) is an insect transmitted viral disease of several species of domestic and wild ruminants. The disease is a cause for serious concern to livestock industry due to staggering direct and indirect economic losses. In many countries like India having considerable sheep population, the disease has become endemic. Severity of the infection depends upon the species and breed of animals, serotypes/strains of virus and prevalent ecosystem. The present study was aimed to find out prevalence of Bluetongue virus (BTV) antibodies in cattle and sheep of different regions of the Gujarat State. Avidin-Biotin enzyme-linked immunosorbent assay (AB-ELISA) and indirect enzyme-linked immunosorbent assay (I-ELISA) employed for antibody detection were also compared in terms of their sensitivity and specificity. An attempt was also made to standardize polyacrylamide gel electrophoresis (PAGE) for detecting BTV. Out of 527 cattle and sheep sera tested, 343 (65.08%) and 396 (75.14%) were found to be positive for BTV antibodies by AB-ELISA and IELISA respectively. Specieswise, 69.04 and 77.57 percent of cattle and 55.55 and 63.33 percent of sheep revealed antibodies to BTV by AB-ELISA and l-ELISA respectively. The highest prevalence rate was found in South Gujarat region (67.94 and 78.63 percent) by AB-ELISA and l-ELISA respectively and female animals (65.53 and 77.84 percent) showed more prevalence than male animals (64.35 and 70.79 percent). Districtwise seroprevalence study revealed highest incidence in Valasad district (68.51%), followed by Navsari (67.70%), Kutch (60.74%), Junagadh (57.69%) and Rajkot (51.72%) district by AB-ELISA, whereas IELISA yielded highest prevalence rate in Valasad (80.55%) followed by Navsari (77.82%), Rajkot (69.96%), Junagadh (69.23%)) and Kutch (66.96%>) district. In cattle, highest incidence was observed in Kankrej (75.52%)) by ABELISA, while HF cross-yielded highest positive samples (84.72%) by l-ELISA. Lowest incidence was seen in Gir breed by both the tests (51.51 and 66.66 percent), while in sheep, higher prevalence rate in native Patanwadi breed (64.28 and 73.21 percent) was observed than the crossbred (41.17 and 47.05 percent) by AB-ELISA and I-ELISA respectively. Comparison of l-ELISA and AB-ELISA for the detection of BTV antibodies, revealed that the former test was better in terms of sensitivity and specificity. Out of a total 527 serum samples tested 343 (65.08%) and (75.14%) reacted positively in AB-ELISA and l-ELISA respectively. l-ELISA detected BTV antibodies in 54 (10.25%) samples, which were negative by AB-ELISA. Relative sensitivity and specificity of AB-ELISA to l-ELISA were 86.63 and 99.23% respectively and overall agreement between both the tests was 89.56%. RNA-PAGE was employed for detecting BTV using a cell culture propagated BTV serotype 1. This was attempted essentially to standardize this highly sensitive technique, so as to use if routinely in future for the field samples. The study revealed 10 RNA segments with a migration pattern resembling to serotype 1 of BTV.