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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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Subject: Veterinary Microbiology × End date: 1998 × Type: Thesis ×
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Now showing 1 - 9 of 26
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    ThesisItemOpen Access
    ISOLATION AND CHARACTERISTICS OF Staphylococcus aureus FROM BOVINE MILK
    (AAU, Anand, 1990) Purohit, Jayantilal Hargovind; JHALA, V. M.
    The present study was undertaken with a view to know the occurrence of Staphylococcus aureus In bovine milk In relation to species, managemental conditions, breed, method of milking, parity, stage of lactation and Involvement of the quarters as well as to observe the relationships among the certain characteristics, Including enterotoxigenicIty, of S.aureus. The isolates were also phage typed to know the possible origin. The milk samples were collected from the animals maintained at six different farms comprising of four GAU farms and two private farms. The cows were maintained at four farms whereas buffaloes were maintained at remaining two farms. A total of 925 milk samples (758 from cows and 167 from buffaloes) from the individual quarters of 234 animals comprising of 191 cows and 43 buffaloes were collected and processed for isolation and identification of S.aureus. Of these, 94 quarters (10.16 per cent) of 67 animals (28.63 per cent) revealed the presence of S.aureus. The incidence of S.aureus was more commonly encountered amongst the cows on animal basis (31.94 per cent) as well as on quarter basis (11.35 per cent) than those of buffaloes (13.95 and 4.79 per cent, respectively).
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    ThesisItemOpen Access
    STUDIES ON IMMUNOMODULATION BY LEVAMISOLE ALONG WITH VACCINATION IN CHICKS AGAINST RANIKHET DISEASE AND IN CALVES AGAINST HAEMORRHAGIC SEPTICAEMIA
    (AAU, Anand, 1985) VYAS, GIRISH P.; DHOLAKIA, P. M.
    The present study was aimed assessment of the immunomodulatory effect of levamisole along with vaccination in chicks against RanikhetDisease (R.D.) and in calves against Haemorrhagic Septicaemia (H.S.) in relation to serum antibody titres, effect of dose of levamisole, total immunity period and electrophoretic pattern of serum proteins after immunization and treatment with levamisole. In all 560 serum samples from 140 chicks belonging to Central Poultry Research Station and 100 serum samples from 20 calves belonging to Livestock Research Station of Gujarat Agricultural University, Anand Campus, Anand were subjected to Haemaggulutination Inhibition (H.I.) test, Passive Haemagglutination (PHA) test, Sodium sulfite precipitation test and Agar gel electrophoresis.
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    ThesisItemOpen Access
    STUDIES ON MYCOTIC MASTITIS IN EXOTIC AND CROSSBRED CATTLE WITH SPECIAL REFERENCE TO ITS INCIDENCE AND DIAGNOSIS
    (AAU, Anand, 1984) SIMARIA, MULJI B.; DHOLAKIA, P. M.
    The present study was taken up to assess the incidence of mycotic infections of under in apparently healthy quarters of lactating cows and diagnosis in milk samples from clinical cases, Simultaneously, the prevalence of subclinical mastitis (SCM), and pathogenicity of fungal strains were also studied. The milk samples from 150 lactating cows (53 Jersey, 30 Kankrej and 67 crossbred) were collected. In addition to this 82 milk samples from clinical cases of mastitis received in Department of Bacteriology were also included in the present study. Subclinical mastitis (SCM) was recorded as 15.33 and 5.27 per cent on animal and quarter basis with California Mastitis Test. Although, incidence of SCM was highest in Jersey herd (8.82% quarterwise), fungal isolation was as low as 2.45 per cent, Hence CMT did not prove efficient enough to detect fungal infection in apparently normal udders.
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    ThesisItemOpen Access
    STUDIES ON POSTVACCINAL IMMUNITY TO BRUCELLA ABORTUS IN VACCINATED EXOTIC CATTLE
    (AAU, Anand, 1987) RAJPUT, H. A.; DHOLAKIA, P. M.
    The disease brucellosis caused by the infection of Brucella abortus in cattle adversely affects the economy of livestock rearing due to losses of calves created by abortions in suffering animals along with loss in milk production. The disease is world wide in distribution and calves lost due to abortion creat adverse economic impact on the animal husbandry development. Being zoonotic in nature the disease also occupies a special importance. It is therefore essential to diagnose the disease in herd as early as possible for its effective control. It is now well-established that the brucella organisms being the facultative intracellular organism the cellular immunity also plays a major role in the host resistance in addition to humoral immunity.
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    ThesisItemOpen Access
    STUDIES ON ADVANCED METHODS OF ASCERTAINING BRUCELLOSIS IN CATTLE
    (AAU, Anand, 1985) PATEL, J. B.; JHALA, V. M.
    The disease Brucellosis caused by the infection of Brucella abortus in cattle adversely affects the economy of livestock rearing due to losses of calves created by abortions in suffering animals along with loss in milk production. The disease is worldwide in distribution and calves lost due to abortions create highly adverse economic impact on the animal husbandry development. The disease also occupies a special importance being zoonotic in nature. It is# therefore, essential to diagnose the disease in a herd as early as possible for its effective control. It is now well established that the Brucella organisms being the facultative Intracellular organisms the cellular immunity plays a major role in the host resistance. In view of the above facts, the present work was carried out in cows at Livestock Research Station, Gujarat Agricultural University, Sardar Krushinagar, employing following six parametars to study their efficacy. (1) Screening of individual cow by milk ring test using ABR i.e. Aboirtus Bang Ring Antigen. (2) Above cows and the cows which were not in milk but having the history of abortion, were subjected to serum plate agglutination test. (3) All above sera samples were further examined by Serum Tube Agglutination Test (STAT). (4) Fourteen sera samples from positive, doubtful and negative reactors to STAT were sent to International Brucella Laboratory, Indian Veterinary Research Institute, Izatnagar for confirmation by complement fixation test. (5) Determination of conglutinin (K) and immunoconglutinin (IK) level in sera of positive, doubtful and negative reactors to STAT . (6) Measurement of cell mediated immunity in vitro by leucocyte migration inhibition test. Prom the results obtained it could be concluded that:- i)The serological tests namely milk ring test plate agglutination test, tube agglutination test may serve as useful tool for diagnosis of brucellosis in a herd. ii) The level of K and IK in sera of positive, doubtful or negative reactors to STAT have no significant difference and there was no significant correlation with agglutination titre, hence the determination of K and IK may not be of use in diagnosis of Brucellosis. iii) The Brucella organisms are being the facultative intracellular micro organisms, the cell mediated immunity plays an important role in resistance. The measurement of cell mediated immunity in vitro by leucocyte migration inhibition test led to the conclusion that this test may be of very great value as a modern advanced method for the diagnosis of Brucellosis where cellular immunity is considered to play a significant role.
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    ThesisItemOpen Access
    STUDIES ON MICROFLORA OF BOVINE SEMEN AND THEIR EFFECT ON SEMEN QUALITY
    (AAU, Anand, 1983) KHER, HIRABHAI N.; DHOLAKIA, P. M.
    Tho present study ims alaed to assess the baoterial load and types of organisms presant In saaien and their relation to semen quality. The study also included antibiotie sensitivity pattern of the isolates in vitro. Totally 45 bulls belonging to (a) Regional A. I. Centre, Rajkot, (b) Central Semen Collection Station, Mehsana, and (c) A. I. Centre, Godhra were studied during the year 1982-83. Bacterial load in the range frota 630 to 14375 organisms per ml for neat semen and in the range from 12300 to 130000 per ml for preputial washings had been encountered. The bacterial load from neat semen was high in summer season followed by monsoon and winter season, while that of preputial washings was high in monsoon season followed by summer and winter season. The semen quality was found fairly good , irrespective of bacterial load of ejaculates. Within the centre, bacterial load of neat semen and preputial washings was higher in buffalo bulls than that of cow bulls and was not varying according to type of sheath.
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    ThesisItemOpen Access
    ON THE OCCURRENCE OF GRAM NEGATIVE BACTERIAL INFECTIONS IN HATCHERIES WITH AN ATTEMPT TO ISOLATE SALMONELLA ORGANISMS
    (AAU, Anand, 1988) HATHI, A. V.; Dholakia, P. M.
    Present study was aimed to isolate and identify the bacterial organisms with emphasis on gram negative bacteria present in cloacal swab of appeirently healthy layers, droppings and litter samples of layer house, poultry feeds, fluff in the hatohery, air of the hatchery and fresh egg shell + CAM. The study also included serotyping of E.coli cultures and antibiotic sensitivity pattern of Escherichia ooli isolates in vitro. Totally 47, 51 and 65 samples of various sources were collected from the (A) Intensive Poultry Development Block, Makarba (Ahmedabad), (B) I.P.D.B., Baroda and (C) I.P.D.B., Surat, respectively. Of 47 collected samples of I.P.D.B. Makarba (Ahmedabad), yielded 90 bacterial isolates while of 51 samples of I.P.D.B. Baroda, 37 samples were sterile for bacterial organisms. Rest of the samples yielded 28 bacterial isolates whereas 65 samples of I.P.D.B. Surat yielded 107 bacterial isolates.
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    ThesisItemOpen Access
    SEROPREVALENCE AND DIAGNOSIS OF BLUETONGUE VIRUS BY REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION
    (AAU, Anand, 1998) Hinsu, T. V.; Kher, H. N.
    Bluetongue (BT) is an insect transmitted viral disease of several species of domestic and wild ruminants. The disease is a cause for serious concern to livestock industry due to staggering direct and indirect economic losses. In many countries like India having considerable sheep population, the disease has become endemic. Severity of the infection depends upon the species and breed of animals, serotypes/strains of the virus and prevalent ecosystem. The present study was aimed to find out prevalence of Bluetongue virus (BTV) antibodies in domestic ruminants and of Epizootic haemorrhagic disease virus (EHDV) antibodies in cattle of Kutch district of Gujarat state. Agar gel immunodiffusion (AGIO) and competitive enzyme-linked immunosorbent assay (c-ELISA) employed for antibody detection were also compared in terms of their sensitivity and specificity. An attempt was also made to standardize Reverse transcriptase-polymerase chain reaction (RT-PCR) for detecting BTV nucleic acid. Out of 162 sera tested, 87 (53.70%) and 126 (77.78%) were found to be positive for BTV antibodies by AGID and c-ELISA respectively. Specieswise, 24.56 and 63.16% of sheep, 80.00 and 96.36% of goats and 58.00 and 74.00% of cattle revealed antibodies to BTV by AGIO and c-ELISA respectively. The highest prevalence rate was found in Northern-east region (70.69 and 86.21%), followed by Central (48.72 and 75.64%) and Southern-west region (30.77 and 65.38%) of the Kutch district, respectively by AGID and c-ELISA. Female animals (63.03 and 86.81%) showed more prevalence than male animals (36.63 and 66.20%), as determined by AGID and c-ELISA respectively. In sheep, higher prevalence rate in native Patanwadi breed (47.06 and 86.16%) was observed than the crossbreds (11.76 and 43.48%) by AGID and c-ELISA respectively. A total of 50 cattle sera tested for BTV antibodies were also tested for EHDV antibodies by EHDV-AGID test. Of these, 9 (18.00%) were found positive for EHDV antibodies. Amongst these positive sera, five reacted specifically to EHDV antigen, without crossreacting to BTV antigen. Comparison of c-ELISA and AGID tests for the detection of BTV antibodies, revealed that the former test was better in terms of sensitivity and specificity. Of the total 162 serum samples, 87 and 126 samples reacted positively in AGIO and c-ELISA respectively. Eighty six samples were found positive and 35 negative by both the tests; while 40 samples detected positive by c-ELISA were negative by AGIO. Only one sample reacted positively to AGIO but negative in c-ELISA. This sample turned out to be positive for EHDV antibodies. Relative sensitivity and specificity of AGIO to c-ELISA were 68.25 and 97.22% respectively and overall agreement between both the tests was 74.69%. RT-PCR was employed for detecting BTV nucleic acid using BTV groupspecific segment 7 prime and BHK-21 cells adopted BTV serotype 1. This was attempted essentially to standardize this highly sensitive technique, so as to use it routinely in future for the field samples. The study revealed an amplified product of 500 bp specific to the primer used in the study.
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    ThesisItemOpen Access
    SEROEPIDEMIOLOGY, ISOLATION AND PATHOGENICITY OF BLUETONGUE VIRUS
    (AAU, Anand, 1996) Chandel, Bharat Singh; Kher, H. N.
    Bluetongue (BT) is an infectious, non contagious disease of domestic and wild ruminants. Bluetongue virus ( BTV ) causes severe disease in sheep, which i s transmitted by insect vectors (Culicoides spp.) . The ability of BTVs to inflict pathological changes in susceptible sheep depends on the virulence of a particular viral isolate , susceptibility of the host and a number of environmental factors related to climatic conditions. The present study was aimed at the seroepidemiology, prevalence of BTV serotypes in sheep, isolation, propagation and identification of local isolates and pathogenicity of BTV in natural and experimental cases of sheep. This study also covered the seroprevalence of epizootic haemorrhagic disease virus (EHDV) in cattle and buffaloes as it is related to orbivirus group. A seroepidentiological survey of BTV precipitating antibodies was carried out by agar gel immunodiffusicm ( AGID ) test in different species of livestock in Gujarat. Out of 1623 sera tested, 407 (25.07%) were found to be positive for BTV antibodies.