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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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Subject: Veterinary Microbiology × Author: PATEL, RITESHKUMAR NARSINHBHAI × End date: 2017 × Start date: 2016 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    SERODETECTION OF Brucella ANTIBODIES AND CHARACTERIZATION OF Brucella ISOLATES FROM REPRODUCTIVE DISORDERS OF CATTLE AND BUFFALOES IN AND AROUND ANAND
    (AAU, Anand, 2016) PATEL, RITESHKUMAR NARSINHBHAI; Roy, Ashish
    Brucellosis is a zoonotic infection caused by gram negative, facultative intracellular bacteria Brucella that are pathogenic for a wide range of animals and human beings. The disease has a considerable impact on human and animal health, as well as socioeconomic impacts, especially in which rural income relies largely on livestock breeding and dairy products. Brucella organisms are coccobacilli or short rods, arranged singly and less frequently in pairs or small groups. The genus Brucella belong to the aproteobacteria group and consists of six recognized species: Brucella abortus, Brucella melitensis, Brucella suis. Brucella ovis. Brucella canis, Brucella neotomae and the strains recently discovered in marine mammals and in common vole (Microtus arvalis) and published under the respective species names oi Brucella ceti, Brucella pinnipedialis and Brucella microti. Diagnosis is usually based on serological tests and/or cultivation. Serological evidence suggested that brucellosis is highly endemic in many parts of India. The present study was undertaken to detect Brucella antibodies in serum, isolation of Brucella organisms from various reproductive disorders (abortion, R.O.P., endometritis, infertility and repeat breeder) and PCR based identification and characterization of Brucella isolates. For detection of Brucella antibodies in serum, two serological methods viz., i-ELISA and RBPT were carried out. For isolation of Brucella organisms Brucella agar medium (BAM) was used and for detection oi Brucella DNA by PCR three different genus specific primer pairs viz., B4/B5, JPF/JPR and F4/R2 were used. Species identification of various brucella isolates obtained from cattle and buffalo were carried out by AMOS PCR and Bruce-ladder PCR. During the study period, total 434 sera samples comprising of 361 from cattle and 73 from buffaloes were screened using i-ELISA, of which 51 (14.12%) and 22 (30.13%) were found positive, respectively. The overall seropositive sera among 434 sera samples was found to be 73 (16.80%). In present study, seroprevalence of Brucella antibodies from gynaecological disorder cases was studied from the 434 serum samples comprises of 361 from cattle and 73 buffaloes by RBPT. Forty eight sera samples were found to be positive by RBPT indicating seropositivity of 11.05%. Among the two serological tests employed for detection of Brucella antibody in serum of cattle and buffaloes, the highest positive results were obtained by ELISA (16.80%), followed by RBPT (11.05%). Prevalence of Brucella antibody in abortion and R.O.P. were studied using i- ELISA and RBPT, recorded 34.69% and 22.44% positivity in cattle and buffaloes, respectively whereas from reproductive disorders (endometritis, infertility and repeat breeder) 11.69% by i-ELISA and 7.25% by RBPT were found to be overall seropositive in cattle and buffaloes. Attempt for cultural isolation was done by inoculating 114 various samples like vaginal swabs, aborted material, milk and placenta. Of these, only 3 samples yielded Brucella organisms on Brucella agar medium. The isolates were identified by cultural, morphological, biochemical characteristics test and further confirmed by PCR using different genus and species specific primer pairs. Colony PCR was carried out using various genus specific primer pairs for the confirmation oi Brucella isolates for different gene. The desired product of 223 bp using B4/B5 (BCSP31 gene) primer pair was amplified in all the 3 isolate (including reference strain). However, desired product of 193 bp using JPF/JPR (omp2 gene) primer pairs was amplified except isolates C2 (cow). Whereas product of 905 bp using F4/R2 (16S rRNA gene) primer pairs was amplified in all the isolates. Furthermore for the species identification, multiplex PCR assays were employed that were AMOS PCR and Bruce-ladder PCR. AMOS PCR could amplify B. abortus specific primer, whereas Bruce-ladder also could able to identify all 3 isolates as B. abortus. All the isolates of Brucella abortus from cows (C1, C2 and C3) were found to be 100% sensitive to Streptomycin, Tetracycline, Amikacin, Erythromycin, Pefloxacin, Amoxyclav, Spectinomycin and Norfloxacin whereas all the three B. abortus isolates were found to be resistant to Ampicillin.