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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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20011
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Subject: Veterinary Microbiology × Author: Anand, Sanjeev Kumar × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    MOLECULAR CHARACTERIZATION OF ESCHERICHIA COLI STRAINS USING PCR-SSCP AND HETERODUPLEX ANALYSIS
    (AAU, Anand, 2001) Anand, Sanjeev Kumar; Purohit, J. H.
    The present study was undertaken with a view to characterize and classify the various strains of E.coli on the basis of SSCP and heteroduplex analysis. DNA from 150 isolates of E.coli, collected earlier from autopsied birds, was extracted using either lysozyme or proteinase K method and V3 region of 168 rRNA gene was amplified by PCR using universal primer designed for that region. The PCR product was subjected to SSCP and heteroduplex analysis on different gel matrices. Five different gel matrices were tested to find out suitability for SSCP and heteroduplex analysis. Among the gels tested 0.5X MDE with 10% glycerol gave the best results. Twenty two different SSCP band patterns were obtained by electrophoresis of ssDNA on non-denaturing gel matrix. The bands of ssDNA in these patterns corresponded to 983 to 379bp equivalent mobility of dsDNA ladder. These patterns contained a minimum of two ssDNA bands to maximum seven. Total 25 bands were found. On heteroduplex analysis keeping the target DNA of Proteus vulgaris as constant, 13 distinct band patterns were observed. The bands of DNA in these patterns corresponded to 900 to 331bp equivalent mobility of dsDNA ladder. These patterns contained a minimum of three to maximum seven heteroduplex bands. The total 17 bands were observed. No relationship could be observed amongst the serotypes, biotypes and SSCP band patterns of E.coli studied. Thus PCR-SSCP and heteroduplex analysis can be used independently for the analysis of the bacterial communities.