Loading...
Anand Agricultural University, Anand
Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur
AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.
Browse
71 results
Search Results
ThesisItem Open Access ASSESSMENT OF GENETIC VARIABILITY AND CHARACTER ASSOCIATION STUDIES IN Aloe (Aloe barbadensis Mill.) FOR AGRO - BOTANICAL AND QUALITY TRAITS(AAU, Anand, 2004) DEORE, ABHIJIT A.; Sriram, S.Aloe (Aloe barbadensis Mill.), one of the priority medicinal plants of commercial importance in India, belongs to the family Liliaceae. Dry weight of juice and aloin content (gm/100 gm dry exudate) are important economic traits of this plant. These traits are manifestations of many contributing characters. As the work on genetics of these traits is scarce in the literature, the present investigation was undertaken. Information was generated on variability, character associations as well as direct and indirect effects of different characters on these economic parameters of Aloe. The field experiment was conducted during January-2002 to February-2003 at the experimental farm of Medicinal and Aromatic Plants Project, Anand Agricultural University, Anand. The experimental material (19 months old) comprised of 23 genotypes. The evaluations were made in completely randomized design. Observations were recorded on randomly selected five plants and each plant was treated as single repeat. Analysis of variance revealed significant differences among the genotypes for all 35 characters studied suggesting presence of good deal of variability in the experimental material. The characters studied were length of inflorescence, number of flowers per inflorescence, density of flowers in an inflorescence, per cent pollen fertility, number of capsules per inflorescence, number of seeds per inflorescence, number of seeds per capsule, per cent seed germination, leaf length, leaf breadth, leaf thickness at the base, number of leaves per plant, weight of single leaf, weight of gel, volume of gel, dry gel content (gm/100 ml juice), fresh weight of juice, dry weight of juice, moisture per cent in juice, fresh weight of exudate, dry weight of exudate, moisture per cent in exudate, dry exudate content (gm/100 ml exudate), cold extracted gel (gm/100 ml juice), cold extracted gel (gm/100 gm gel powder), hot extracted gel (gm/100 ml juice), hot extracted gel (gm/100 gm gel powder), aloin content (gm/100 gm fresh exudate), aloin content (gm/100 gm dry exudate), aloin content (gm/100 gm fresh juice), aloin content (gm/100 gm gel powder), barbaloin content (gm/100 gm fresh exudate), barbaloin content (gm/100 gm dry exudate), barbaloin content (gm/100 gm fresh juice) and barbaloin content (gm/100 gm gel powder). Characters, length of inflorescence, number of flowers per inflorescence, density of flowers, per cent pollen fertility, number of seeds per inflorescence, per cent seed germination, weight of leaf, weight of gel, volume of gel, fresh weight of juice, dry weight of juice, fresh weight of exudate, dry weight of exudate, cold and hot extracted gel in juice, cold and hot extracted gel in powder showed predominance of genotypic variance and also showed high magnitude of broad sense heritability coupled with high genetic advance. Hence, it is concluded that experimental material possessed potential for improvement in these traits. Correlation matrix revealed that all character paired were positively and significantly correlated with each other both at genotypic and phenotypic levels but for number of flowers per inflorescence and number of leaves per plant which were negatively correlated. Characters, length of inflorescence, number of flowers per inflorescence, leaf length, leaf breadth, leaf thickness at the base, weight of single leaf, weight of gel and fresh weight of juice showed their highly significant and high magnitude positive association with both the economic parameters viz., dry weight of juice and aloin content (gm/100 gm dry exudate). Hence, based on high magnitude correlation coefficient with economically important characters, it could be concluded that certain inflorescence and leaf characters could be exploited for improvement in this crop. Path analyses revealed that leaf thickness at the base and weight of gel had positive direct effect on both the economic parameters i.e. dry weight of juice and aloin content (gm/100 gm dry exudate). Other characters, length of inflorescence, number of flowers per inflorescence, leaf length, leaf breadth, weight of single leaf and fresh weight of juice had direct but negative effect on these economic traits. Though their correlation with economic traits was positive and significant, their direct and indirect effect on both the economic parameters was variable. In general, characters leaf thickness at the base and weight of gel, with high magnitude of direct effects may be helpful to select the elite genotypes for improvement in these two economic traits i.e. selection could be based on visibly assessable character leaf thickness at the base and later results could be confirmed in laboratory, testing for gel weight. Based on these analyses and mean values, eight elite genotypes viz., IC-112522, IC-112527, KM 12535, IC-112528, KM 11269, KM 12531, Bitter rayan and Sweet rayan were identified for further use in crop improvement programme.ThesisItem Open Access GENETIC ARCHITECTURE OF GREEN FRUIT YIELD AND QUALITY CHARACTERS IN RELATION TO GMS BASED HYBRIDS IN CHILLI (Capsicum annuum var. longum (D.C.)Sendt.)(AAU, Anand, 2004) PATEL, PRAGNESH R.; Patel, J. A.The present investigation comprised of 40 F1S developed from four Gms lines and ten male parents through Line x Tester mating design in chilli (Capsicum annuum var. longum (D.C.) Sendt.) was undertaken at Main Vegetable Research Station, Gujarat Agricultural University Anand during kharif planting crop season of the year 2002-03. The experimental material (40 F1S, 4 Gms lines, 10 pollen parents and one check variety) was evaluated in complete randomized block design with three replications. The main objective of the experiment was to study heterosis, gene effects and combining ability effects for green fruit yield and various yield as well as quality characters. Analysis of variance indicated presence of considerable amount of variability among genotypes for all the fourteen characters studied. Mean squares due to parents and hybrids were significant for all the characters. Whereas, parents was. hybrids contrast comparison was significant for all the attributes except average fruit weight, suggesting the existence of differences between parents and hybrids leading to evidence for presence of heterosis for all those characters. In respect to per se performance of parents, among the Gms lines ACMS-3 and ACMS-4 and among the male inbreds ACS 97-2, ACS 2000-01. ACS 2000-02, ACS 2000-03, Punjab Gucchedar and RHRC Pendent were found to be superior for green fruit yield and most of the yield as well as quality characters. Among the hybrids. ACMS-2 x Pb. Gucchedar. ACMS-3 x Pb. Gucchedar. ACMS-3 x ACS 97-2, ACMS-4 x Arka lohit and ACMS-2 x ACS 2000-02 had high per se mean for green fruit yield. These hybrids also possessed high mean values for other important characters in respect to yield and quality. In the present investigation, significant and higher estimates of relative heterosis, hetcrobeltiosis and standard heterosis were observed for green fruit yield. The hybrids ACMS-2 x Pb. Gucchedar. ACMS-4 x ACS 2000-03, ACMS-4 x ACS 2000-01 and ACMS-3 x Pb. Gucchedar exerted greater amount of heterobelliosis and standard heterosis for yield, these hybrids also depicted larger estimates of heterosis for number of fruits per plain. These lop ranking hybrids also registered significant desirable heterosis for most of the growth and quality characters in addition to important yield contributing component characters. For number of fruits per plant, hybrid ACMS-3 x Pb. Gucchedar borne the highest fruits followed by ACMS-4 x Arka lohit and ACMS-2 X Pb. Gucchedar. likewise these hybrids also depicted the maximum values of standard heterosis and larger estimates of heterobeltiosis for the said trait. These hybrids also registered higher estimates of various heterotic effects for one or more than one yield contributing component characters. In respect to earliness, hybrids ACMS-: X ACS 2000-03. ACMS-4 x ACS 2000-03 and ACMS-1 x ACS 96-33 look the minimum days for flowering. hence these hybrid had the least estimates of standard heterosis. The estimates of components of genetic variance revealed the importance of non additive gene effect for genetic control of green fruit yield and its important component character number of fruits per plant, for all the growth attributes except plant height and for all the quality parameters. Additive gene effect was importance for fruit length and fruit diameter. Whereas, both the components of genetic variance were significant for average fruit weight. For plant height and fruit shape index none of the gene effects had significant estimates.ThesisItem Open Access DIALLEL ANALYSIS FOR YIELD, ITS COMPONENTS AND QUALITY TRAITS IN ROUND FRUITED BRINJAL [Solanum melongena L.](AAU, Anand, 2003) PATEL, NISHABAHEN B.; Kathiria, K. B.Brinjal is an important solanaceous vegetable crop. Due to wide variability, it holds potential for fiirther improvement. The present investigation on brinjal comprised of a half-diallel set of 8 parents, 28 F1 hybrids and 2 hybrid check varieties GBH-1 and GBH-2. The experiment was laid out in a randomized block design with 3 replications at Main Vegetable Research Station, Gujarat Agricultural University, Anand Campus, Anand, during kharif-rabi 2001-2002. Heterosis, combining ability and components of genetic variance and graphical analysis were studied for 14 characters, viz. days to first picking, plant height, plant spread, primary branches per plant, fruit length, fruit girth, fruit weight, fruits per plant, fruit yield per plant, 100 seed weight, fruit volume, dry matter, total soluble sugars and total phenols. The analysis of variance indicated significant differences among genotypes for all the characters. Several hybrids exhibited heterobeltiosis and economic heterosis for fruit yield and other characters. The hybrids worth for commercial exploitation of heterosis on the basis of both per se performance and estimates of heterosis were AB-00-6 x AB-01-4, PLR-1 x SKN-GR-1 and PLR-1 X Green Round. The general and specific combining ability variances were highly significant for all the traits. The sea and gca variances suggested predominant role of non-additive gene effects for the inheritance of all the traits except fruits per plant. The estimates of general combining ability effects revealed that parents AB-00-6 and PLR-1 appeared to be good general combiners for fruit yield per plant and some of the other yield attributes and quality parameters. A close relationship was found between per se performance and gca effects of parents. The estimates of specific combining ability effects indicated that cross combinations AB-00-6 x AB-01-4, PLR-1 x SKN-GR-1 and PLR-1 x Green Round were significant for fruit yield and its major components as well as quality characters. There was some degree of correspondence between per se performance and sea effects of hybrids as well as sea effects and heterobeltiosis for most of the traits. Assumptions basic to diallel were only fulfilled for the characters plant height and fruits per plant. The component and graphical analysis also revealed the importance of both additive and non-additive genetic variances for the inheritance of most of the traits. Degree of dominance indicated over-dominance for plant height while almost complete dominance was observed for fruits per plant. Positive and negative alleles were distributed asymmetrically and higher frequency of dominant genes were found in the parents for plant height and fruits per plant. The heritability estimates were observed to be high for fruits per plant and moderate for plant height. The graphical analysis indicated complementary type of gene interaction for the control of few traits. The findings have been discussed in the light of their implications in brinjal breeding programme. Present findings indicated that breeding approaches like biparental mating followed by recurrent selection, diallel selective mating etc., in addition to conventional methods are suggested for the improvement of these traits.ThesisItem Open Access HETEROSIS, CORRELATION AND PATH ANALYSIS IN BRINJAL (Solanum melongena L.)(AAU, Anand, 2004) PATEL, BHAVESHKUMAR RAMANBHAI; Jadeja, G. C.Brinjal {Solarium melongena L. 2n=24) also known as egg plant is an important vegetable crop of India. In India, brinjal occupies an area of about 0.51 million hectare with production of 8.80 million tonnes. In Gujarat, it is cultivated in 31,183 hectares giving a production of 5,43,076 tonnes. Inspite of a large number of varieties available in India, only a few are promising, and generally they are improved varieties. Though the wide genetic variability is available in this crop, it has not been utilized and exploited fully. To maintain the uniformity of fruits and to increase yield levels development of hybrid varieties as well as improved varieties having high yield with desired morphological and quality characters is also an urgent need of recent time. Keeping in view the importance of brinjal and need for its improvement, an experiment was conducted to analyze the extent of relative heterosis, heterobeltiosis and standard heterosis among thirty-one hybrids developed from fourteen selected diverse parents. Further the developed genotypes were subjected for character association study to know the association of various agronomically important and some quality traits viz., days to flowering, average fruit weight, fruit length, fruit girth, number of fruits per plant, number of functional branches, plant height, moisture, dry matter, total soluble sugar and phenol with fruit yield per plant and also among themselves. These genotypes were grown in 7 x 7 simple lattice design with two replications. The experiment was conducted at Main Vegetable Research Station, Gujarat Agricultural University, Anand Campus, Anand during kharif- rabi 2001-2002. Out of thirty-one crosses studied, extent of relative heterosis, heterobeltosis and standard heterosis were computed using the mean values. Highest amount of relative heterosis was recorded by cross Doli-5 x AB-99-4 for average fruit weight, followed by cross KS-331 x AB-99-4 for fruit yield per plant. Study on heterobeltiosis and relative heterosis showed similar findings. In both the cases the cross Doli-5 x AB-99-33 reported the highest value for the average fruit weight followed by cross Doli-5 x AB-99-33 for average fruit length. The highest expression of standard heterosis was found for cross Doli-5 x GP-217 for number of fruits per plant followed by cross BB-71 x AB-99-33 for phenol content. Hybrid BB-71 x Green Castor long was found to be a good as it exhibited moderate, high and very high significant to highly significant values of heterosis, heterobeltiosis and standard heterosis for different traits studied except for moisture content. Parent BB-71 was found to be a very promising combiner as it's crosses showed significant and highly significant values for most of the traits under study, this suggests the need to exploit the present material for combining ability study to work out good breeding plan for the improvement of the present status of the crop. Correlation coefficient analysis reported that, only days to flowering was having desirable significant effect on fruit yield per plant in negative direction, whereas all the other traits exhibited non significant association. Number of functional branches showed highly significant positive correlation with number of fruits per plant and significant positive correlation with fruit length respectively. Path coefficient analysis based on genotypic correlation revealed that days to flowering is the major yield attributing trait, suggesting earliness as the highly influencing trait to be considered as very important trait for selecting high yielding genotypes. The indirect effect of number of functional branches on fruit yield via number of fruits per plant was moderate and coupled with highly significant positive correlation value, indicated the importance of selection of highly functional branched genotypes. The finding of the present investigation lead to the conclusion that the scope of improving the present status of brinjal crop through heterosis breeding is high. Earliness coupled with high functional branching habit of the plant is the desirable ideotype to be selected as per the character association study for effective results.ThesisItem Open Access LINE X TESTER ANALYSIS FOR YIELD AND QUALITY TRAITS IN RED CHILLI (Capsicum annuum var. longum (DC) Sendt.)(AAU, Anand, 2004) SHAH, KEYUR ASHWINKUMAR; Bhalala, M. K.Chilli is an important vegetable crop from Solanaceae family. Due to wide variability, it holds potential for further improvement. The present investigation on chilli comprised of 4 GMS based females, 9 males, 36 F1 hybrids and 2 check varieties S-49 and Jwala. The experiment was laid out in randomized block design with 3 replications at Main Vegetable Research Station, Gujarat Agricultural University, Anand Campus, Anand, during kharif-rabi 2002-2003. Heterosis, combining ability and components of genetic variance were studied for 10 characters viz. days to flower initiation, plant height, fruit length, fruit girth, fruits per plant, fruit weight, red ripe fruit yield per plant, ascorbic acid content capsaicin content and moisture content. The analysis of variance indicated significant differences among genotypes for all the characters. Several hybrids exhibited heterobelriosis and economic heterosis for red ripe fruit yield and other characters. The hybrids worth for commercial exploitation of heterosis were ACMS-3 X ACS-2000-2, ACMS-4 X RHRC Pendant, ACMS-4 X ACS-97-2, ACMS-3 X LCA-301 and ACMS-3 X RHRC Pendant. The analysis of combining ability suggested predominant role of non-additive gene action for days to flower initiation, capsaicin content and moisture content. While both additive and non-additive gene actions were observed to be important for the traits namely plant height, fruit length, fruit girth, fruits per plant, fruit weight, red ripe fruit yield per plant and ascorbic acid content. The estimates of gca effects revealed that GMS lines ACMS-3 and ACMS-4 and male parents ACS-2000-2, ACS-2000-3, RHRC Pendant and GC-1 appeared to be good general combiners for red ripe fruit yield per plant and some of the other yield attributes and quality parameters. The estimates of specific combining ability effects indicated that cross combinations ACMS-3 X LCA-301, ACMS-1 X ACS-2000-1, ACMS-4 X ACS-97-2 and ACMS-3 X ACS-2000-2 were good cross combinations for red ripe fruit yield, its major components as well as quality characters. The three hybrids ACMS-3 X ACS-2000-2, ACMS-4 X ACS-97-2 and ACMS-3 X LCA-301, which showed high per se performance, higher heterotic effects and significant sea effects could be exploited for heterosis and for developing high yielding varieties following appropriate breeding methodology.ThesisItem Open Access HETEROSIS AND COMBINING ABILITY IN PEARL MILLET (Pennisetum glaucum (L.) R.Br.)(AAU, Anand, 2004) MISTRY, JIGAR GIRISHKUMAR; Saiyed, M. P.Pearl millet is one of the important staple food crops of India ranking fourth in acreage. It being a drought tolerant crop has become the important component of dry land farming. The present investigation on pearl millet comprised of 5 (CMS) based females, 12 males, 60 F1 hybrids and 3 check hybrids, GHB-526, GHB-558 and MH-169. The experiment was laid out in randomized block design with 3 replications at Plant Breeding Farm, B. A. College of Agriculture, Gujarat Agricultural University, Anand Campus, Anand, during summer 2002-2003. Heterosis, combining ability effects and components of genetic variance were estimated for 14 characters viz., days to 50 per cent flowering, days to maturity, plant height, number of tillers per plant, effective tillers per plant, number of leaves per plant, leaf area index, fodder yield per plant, earhead girth, earhead length, earhead weight, test weight, grain yield per plant and protein content. The analysis of variance indicated significant differences among genotypes, parents and parents Vs hybrids for all the characters. High magnitude of parents Vs hybrids contrast comparisons indicated presence of conspicuous heterosis. Eight hybrids showed positive standard heterosis for grain yield. The highest standard heterosis for grain yield and yield attributes was recorded with hybrid ICMA 98222 x 179 SB followed by JMSA 9701 x J 2405. The perusal of data for combining ability analysis indicated that the variance due to general combining ability was non significant for all the characters, whereas its counter part variance due to specific combining ability was significant for all the attributes except days to 50 per cent flowering, days to maturity and earhead girth. However, the estimates of potence ratio and degree of dominance revealed preponderance of non additive genetic variance for days to maturity, plant height, effective tillers per plant, number of leaves per plant, leaf area index and test weight; whereas, equal importance of both the variances was observed for days to 50 per cent flowering, number of tillers per plant, fodder yield per plant, earhead length, earhead weight, grain yield per plant and protein content The estimates of gca effects revealed that CMS lines ICMA 98222 and JMSA 9701 and male parents 179 SB, 180 SB and J 2405 appeared to be good general combiners for grain yield per plant and some of the other yield attributes and protein content as well. The estimates of specific combining ability effects indicated that, the cross combinations ICMA 98222 x 179 SB, ICMA 97222 x 180 SB, ICMA 2002 X 179 SB, ICMA 9902 x 234 SB and ICMA 98222 x 178 SB were found to be good specific combiners for grain yield, other yield attributes and protein content. The hybrids ICMA 98222 x 179 SB, JMSA 9701 x J 2405, ICMA 97222 x J 2405, ICMA 98222 x J 2405 and ICMA 97222 x 180 SB, showed high per se performance, higher heterotic effects and significant sea effects and hence these hybrids could be exploited for heterosis and for development of high yielding inbreds through appropriate breeding methodology.ThesisItem Open Access GENETIC VARIABILITY AND ASSOCIATION ANALYSIS IN SLENDER GRAIN RICE {Oryza sativa L.)(AAU, Anand, 2005) PRATAPSINH, MAKAWANA BHAVANISINH; Jadeja, G. C.Rice (Oryza sativa L.) is one of the most important staple food crops of tlie world. The nature and magnitude of genetic variability, interrelationships and direct and indirect effects were studied for different yield and quality traits, in different genotypes of slender grain rice. The experiment comprised of slender grain rice, sown in randomized block design with two replications. It was conducted at Main Rice Research Station, Anand Agricultural University, Nawagam, during kharif 2004. Genetic variability, correlation and path coefficient analysis were studied for 25 characters viz., days to 50% flowering, number of tillers per plant, plant height, panicle length, grain yield per plant, 1000-grian weight, hulling per cent, milling per cent, head rice recovery, grain length, grain breadth, grain L/B ratio, kernel length, kernel breadth, kernel L/B ratio, kernel length after cooking (KLAC), kernel breadth after cooking (KBAC), kernel elongation ratio (KER), breadth expansion ratio (BER), water uptake (WU), volume expansion ratio (VER), amylose content, protein content, alkali spreading value (gelatinization temperature) and cooking time. The analysis of variances revealed significant differences among the genotypes for all the characters. All the traits exhibited predominance of genotypic variance in total variance and the magnitude of heritability (broad sense) was also high. Moderate estimates of genotypic coefficient of variances were observed for grain yield per plant, 1000-grain weight, head rice recovery and grain L/B ratio; while the quality components tike kernel L/B ratio, breadth expansion ratio, water uptake, alkali spreading value (gelatinization temperature) and cooking time recorded moderate to high genotypic coefficient of variances. High genetic advance (% of mean) coupled with high heritability, were observed for agro-morphological traits viz., plant height, grain yield per plant, 1000-grain weight and grain L/B ratio; whereas the quality traits viz., kernel LiB ratio, breadth expansion ratio, water uptake, alkali spreading value (gelatinization temperature) and cooking time also showed similar trend. Most of the remaining traits recorded moderate genetic advance coupled with high heritability. The characters like grain yield per plant, test weight, head rice recovery and grain L/B ratio exhibited moderate genotypic coefficient of variances, with high heritability and high genetic advance. Parallel trend was observed for the quality characters like kernel L/B ratio, breadth expansion ratio, water uptake alkali score (gelatinization temperature) and cooking time; which indicated the better scope for improvement of these traits by an effective selection programme. The correlation coefficient analysis indicated significant and positive association of grain yield with plant height and grain breadth. Interrelationships among important yield components viz., panicle length, 1000-grain weight and grain length was also noticed. Hulling and milling per cent exhibited significant positive association with each other and with water uptake and volume expansion ratio. Kernel length an important from traders' viewpoint, showed association with grain length, grain L/B ratio, kernel L/B ratio and kernel length after cooking. The quality components like kernel elongation ratio and water uptake showed significant positive association with kernel length after cooking. The protein percent showed negative correlation with water uptake; while alkali score (GT) and water uptake were positively associated. Alkali score (gelatinization temperature) and cooking time showed strong positive association. The path analysis with grain yield as dependent variable, indicated that the high direct effects were observed for plant height, grain length and head rice recovery; while the indirect effects were predominant for panicle length, test weight and grain breadth. The path analysis for quality components with kernel length after cooking, showed high direct effects of kernel length and kernel elongation ratio; whereas selection for kernel L/B ratio, water uptake, alkali score (gelatinization temperature) and cooking time can indirectly improve the cooking quality. Based on mean values of morphological traits the genotypes IET 19004, IET 19035, IET 190037, lET 19044 and lET 19045 were identified as elite genotypes; while IET 19001, IET 19009 and IET 19035 were superior with respect to the pre-cooking quality traits. The genotypes lET 19023, IET 19033, IET 19035 and IET 19036 were superior genotypes based on post-cooking quality traits.ThesisItem Open Access STUDIES ON VARIABILITY, CORRELATION AND PATH ANALYSIS IN ISMBGOL (Plantago Ovata Forsk.)(AAU, Anand, 2000) Aher, Adhir Raosaheb; Jadeja, G. C.Isabgol {Plantago ovata Fprsk.) is an important medicinal plant. Seed husk is a drug having laxative property. As the information on genetic parameters is meager, an investigation was undertaken to estimate variability, correlations and direct and indirect effects of different characters contributing towards seed yield by path analysis. The experiment was conducted at the farm of Medicinal and Aromatic Plants Project, Gujarat Agricultural University, Anand Campus, Anand during 1998-99 Rabi season. The experimental material comprised 64 Isabgol genotypes. The experiment was conducted in 8x8 simple lattice design with two replications. Out of sixteen characters studied, eleven characters viz., days :o 50% flowering, days required to complete anthesis, days to maturity, average length of spike, average plant height, seed yield per plant, number of seeds per spike, 1000 seed weight, harvest index, swelling factor and husk yield per plant showed significant variation among genotypes, while five characters, nam.ely average number of leaves per plant, number of flowers per spike, number of tillers at harvestir.g stage, effective spikes per plant and husk content showed non-significant differences. The five characters viz., days to 50% flowering, seed yield per plant, 1000 seed weight, swelling factor and husk yield per plant showed high genotypic variance as well as high heritability. The plant height also showed high genetic advance. The higher estimates of GCV were obtained for seed yield per plant, husk yield per plant, number of leaves per plant and effective spikes per plant. The characters like seed yield per plant and husk yield per plant exhibited high heritability and high expected genetic advance as per cent of mean, indicating better scope for improvement of characters by effective selection programme. The estimates of correlation coefficient revealed that seed yield per plant showed perfect positive association with husk yield at genotypic level. It also showed positive significant association with all the chaiacters except days to 50% flowering and days to maturity. Days to maturity showed negative correlation with seed yield. On overall basis, the correlation study indicated that three characters, namely plant height, 1000 seed weight and number of seeds per spike were most important characters for increasing seed yield, in turn increasing the husk yield. According to path analysis, the characters like husk yield per plant, harvest index and number of seeds per spike were hiving positive direct effect as well as significant positive association with seed yieM, while days to maturity were having negative direct effect and negative significant association with seed yield. In general, husk yield per plant, harvest index and number of seeds per spike having high direct effects arid plant height and length of spike with indirect effects may be helpful in selecting the elite genotypes for further treeding programme. Based on mean performance thirteen elite genotypes viz., RJ-129 (V5), MSB-2 (V7), MSB-8 (V8), JI- 28 (V29), JI-18 (V31), JI-6 (V32), FR-138 (V33), DRP-72 (V46), G.I.I (V47), DRP-46- 187 (V50), FR-185 (V55), MB-29 (V59) and DPP-73 (V62) were identified for further use.ThesisItem Open Access GENOTYPE-ENVIRONMENT INTERACTION STUDIES IN TOMATO (Lycopersicon esculentum MiLL) UNDER IN VITRO CONDITIONS(AAU, Anand, 2000) Chikkale, Firoz A.; Patel, D. R.Tomato (Lycopersicon esculentum Mill) is an important vegetable crop grown all over the world. Different in vitro techniques are widely attempted in this crop as an aid to conventional breeding. Exploitation of advanced in vitro techniques in this crop needs an efficient regeneration of a cell, tissue or organ into complete plant. Additionally, such a system is necessary for the possible improvement of tomato through transformation technique. Hence, present study was undertaken at Plant Tissue Culture Laboratory, Department of Plant Breeding and Genetics B. A. College of Agriculture, Gujarat Agricultural University, Anand Campus, Anand during 1998-2000, with a view to develop such system for few promising genotypes of tomato under advance evaluation at Vegetable Research Project, Anand. The objectives of the study were (1) to identify the best media for in vitro seed germination, (2) to identify the best explant and genotypes for callusing and organogenesis, (3) to study response of different phytohormones to organogenesis, followed by optimization of culture media for maximum regeneration of plantlets, (4) to study response of different phytohormones for shoot tip culture and induction of rooting and (5) to study acclimatization of in vitro raised plantlets. The characters studied were percentage seed germination, days to callus initiation, percentage callusing, fresh as well as dry weight of callus, callus colour, texture and intensity, biomass increase, number of shoots, length of longest shoot, days to root initiation, number of roots, length of longest root and percentage survival. Three explants viz. cotyledon and hypocotyl for callusing and organogenesis and shoot tip for shoot tip culture experiments were used from 15 days old aseptically grown seedling of four genotypes viz., Pusa Ruby, Junagadh Ruby, ATL-11 and ATL-16. MS (1962) and its modifications, LS (1965) and White's (1963) were used as basal media with addition of various levels of different growth regulators viz., lAA, NAA, BA, IB A, Z and Kn. Vitamins free MS (1962) basal medium appeared as best media for germination of all four genotypes with higher percentage germination (84.17%). Among the lAA : BA and NAA : BA combinations, lAA : BA was found superior for callusing and organogenesis. Hypocotyl exhibited significantly superior performance over cotyledon explant for days to callus initiation, percentage callusing, fresh and dry weight of callus, biomass increase and length of shoot whereas, cotyledon was found better for number of shoots. Significant differences among the genotypes and environments for most of the characters using both the explants indicated genotype specific response to various growth media. Significant G x E interactions suggested that genotypes were highly influenced by differences in growth media with respect to callus initiation, number and length of shoots. In general, all genotypes performed better on MS + 0.1 mgl-1 IAA + 0.25 mgl-1 BA and MS +0.2 mgl-1 IAA + 0.5 mgl-1 BA for callusing and organogenesis, respectively.