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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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Subject: Botany × Author: Shah Kevalkumar P × End date: 2013 × Start date: 2011 ×
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    ThesisItemOpen Access
    Sex determination in pointed gourd (Trichosanthes dioica Roxb.) using RAPD and ISSR molecular markers
    (AAU, Anand, 2013) Shah Kevalkumar P; Dr. K. B. Kathiria
    Pointed gourd (Trichosanthes dioica Roxb.) commonly known as “parwal”or “patal” is one of the most nutritive cucurbit vegetables, holding a coveted position in the Indian market during the summer and rainy seasons. In T. dioica Roxb., male and female plants strictly maintain their respective sexual phenotypes, which indicates presence of a clear genetic basis of differences between them. Dioecy represents an inconvenience in pointed gourd breeding, since at present, there is no dependable method to distinguish male and female plants prior to flowering. Sex type determination in pointed gourd is very important for crop improvement process, because it accelerates identification of the fruitful plants. The use of molecular marker provides a quick and reliable identification of sex types in plants. Therefore, the present study was undertaken to identify marker associated with male and female sex expression trait in T. dioica Roxb., followed by development of SCAR. The screening of genomic DNA samples representative of male and female plants of pointed gourd with RAPD and ISSR primers, was used to discover sex specific PCR amplification product. A total of 104 RAPD primers were used for RAPD analysis, out of which 21 primers gave good results. Among these 21 primers, OPC-05 amplified a band of 1000 bp specific to male lines. This RAPD marker was eluted, sequenced and the sequence was used to design primers for SCAR marker. Simultaneously, a female specific RAPD marker of 300 bp was identified using OPK-07. This product was sequenced and primers were developed to convert this marker in to SCAR. But, it failed to amplify the DNA of female lines. Similarly 25 ISSR markers used in the present study did not give any amplification. From the sequence, a set of five primers was designed to allow amplification of male specific region. Out of five SCAR markers, only two gave amplification. But, only single SCAR (SC F/R-3) was used to amplify a product size of 628 bp in male specific DNA as another one was amplified in both the sex.