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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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2002 - 20063
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Subject: Biochemistry Ɨ End date: 2009 Ɨ Start date: 2000 Ɨ Type: Thesis Ɨ Thesis Type: M.Sc Ɨ
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Now showing 1 - 3 of 3
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    ThesisItemOpen Access
    SALICYLIC ACID-INDUCED BIOCHEMICAL CHANGES AGAINST ROOT-KNOT NEMATODE (Meloidogyne incognita) IN CHICKPEA, Cicer arietinum (L.)
    (AAU, Anand, 2002) ARUNA, K. V. S.; TALATI, J. G.
    Investigations were carried out at the Departments of Biochemistry and Nematology, B.A.College of Agriculture, G.A.U., Anand during rabi 2001-2002, to study the effect of exogenous salicylic acid on the induction of resistance through biochemical changes in chickpea, Cicer arietinum (L.) against root-knot nematode (Meloidogyne incognita). Study on development of PR proteins by exogenous SA revealed that SA applied as seed treatment was found to be more effective in inducing PR proteins in chickpea seedlings compared to foliar spray or their combinations. Another experiment was conducted to study the effect of SA on different resistance inducing biochemical parameters viz., sugars, amino acids, proteins, phenols and enzymes using three methods of SA application viz., seed treatment, seed treatment + spray and foliar spray. The results conclusively proved that although the application of SA in all the three methods, effectively enhanced production of all biochemical parameters under study in chickpea. However, seed treatments produced more significant effects than the other treatments. Among the different concentrations used under each method of application, 1500 ppm was more effective followed by 1000 ppm and 500 ppm. This implies that resistance in plant against M. incognita increases with increase in concentration of SA, irrespective of the method of application. Enzyme activities of peroxidase and polyphenol oxidase increased more at later stages of growth (45 & 60 DAS) in contrast to enzyme activities of phenylalanine amonia lyase (PAL), Tyrosine ammonia lyase (TAL) and Ī²-glucosidase in the initial growth stages (30 & 45 DAS). Total soluble sugars decreased with increase in plant age whereas free amino acids, proteins and phenols increased with plant age. No isozymes of peroxidase were detected in both root and leaves due to the SA treatments. All these biochemical changes brought about by the application of SA induced resistance in chickpea against M. incognita which was observed by a decrease in the root-knot index. The third experiment organized to study the effect of SA in inducing resistance at two inoculum levels of M. incognita, showed no significant variation in total phenols and enzyme activities of peroxidase, polyphenol oxidase and phenylalanine ammonia lyase with increase in the inoculum level. The decrease in the root-knot index due to the SA treatments was more at lower inoculum level (1000 J2/plant) than at higher inoculum level (10,000 J2/pIant). All the three experiments conclusively proved that exogenous SA induced plant defence mechanism in chickpea by various biochemical changes which effects the life cycle and development of M. incognita.
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    ThesisItemOpen Access
    BIOCHEMICAL TECHNIQUES FOR RICE (Oryza sativa L.) VARIETY IDENTIFICATION
    (AAU, Anand, 2004) SINGH, DIWAKAR; Talati, J. G.
    Investigations were carried out at the Departments of Biochemistry, Agriculture Botany & Biotechnology, B.A. College of Agriculture, A.A.U., Anand and Department of Animal Genetics & Breeding, College of Veterinary Sciences, A.A.U., Anand, to study and compare biochemical techniques along with traditional techniques for Rice (Oryza sativa L.) variety identification. Study on morphological and chemical tests revealed that it could be useful for grouping of rice varieties/genotypes, while, modified phenol test was useful to identify all fourteen rice varieties/genotypes. Proximate analysis was carried out to study the nutritional composition of milled rice. Nutritional composition showed little variation and thus, was not useful for varietal identification. Peroxidase, polyphenol oxidase and āˆ-amylase enzyme activity showed significant differences and could be applied for varietal identification at 5 & 10 days after germination (DAG), 10 DAG and 20 & 30 DAG respectively, while, acid phosphatase and esterase enzyme activity could not provide useful information regarding varietal identification and thus, was not found useful. Electrophoresis of peroxidase (5 & 10 DAG) and esterase (5, 10 & 20 DAG) isozymes was useful for identification of varieties as well as identification of parents and hybrid and may serve as marker isozyme for rice varieties. Similarly Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of seed storage protein and leaf protein (5 DAG) showed variability and could be effectively useiffor identification of rice varieties/genotypes on the basis of variations in banding pattem and intensity of bands. Randomly amplified polymorphic DNA (RAPD) produced total 180 useful markers of which 77% were polymorphic. All fourteen varieties/genotypes were classified into three different clusters, where variety P-203 was most diverge one. Varieties could be identified on the basis of specific banding pattern. Parents and hybrid also produced differences in banding pattern and could be identified. IR 58025 A line could also be differentiated from its B line on the basis of differences in banding pattern. All the tests conclusively proved that RAPD, SDS-PAGE and isozyme electrophoresis offer a potentially quick, simple, rapid and reliable method for rice varieties/genotypes identification and recognition of lines. Modified phenol test also proved to be rapid and cheap laboratory chemical test for identification of rice varieties, whereas, other chemical and morphological tests can supplement for varietal identification. RAPD and SDS-PAGE were more useful than isozyme electrophoresis because potential number of loci warn more compared to isozymes.
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    ThesisItemOpen Access
    BIOCHEMICAL CHARACTERIZATION AND EFFECTS OF OSMOPROTECTANTS UPON SODIUM CHLORIDE SALINITY STRESS IN RICE CULTIVARS
    (Anand Agricultural University, Anand, 2006) MUNGARA BALAKRISHNA; Dr. Y.M. Shukla
    Salinity is one of the severe abiotic stresses among the different stresses of paddy crop. Four varieties of paddy procured from Main Rice Research Station, Anand Agricultural University, Nawagam were : Jaya, Dandi, CSR-27 ā€“ all tolerant and GR-3 ā€“ susceptible. These varieties were differing in their degree of salt tolerance. All the varieties were grown in earthen pots up to 15 days following the normal practice of pot culture. Fifteen DAG seedlings after gently uprooting were treated with 100, 150 and 200 mM sodium chloride solution for 24, 48 and 72 h time intervals. Various physiological and biochemical observations were recorded for germination percentage, root length, shoot length, seedling vigour index, chlorophyll, total soluble sugars, proteins, SDS-PAGE, proline content, antioxidants enzymes such as super oxidase dismutase, peroxidase, polyphenol oxidase, catalase, esterase and their isoenzymes and sodium, potassium ions