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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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  • ThesisItemOpen Access
    BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF INDIAN MUSTARD [Brassica juncea (L.) CZERN & COSS] GENOTYPES
    (AAU, Anand, 2014) CHAUDHARY, JYOTSANABEN NARSINHBHAI; Y. M., Shukla
    Brassica is an agriculturally important genus containing species with highly diverse morphology and wide ranging utility. Brassica juncea L. is an important oil seed crop which belongs to family Brassicaceae. Brassicajuncea is one of the major sources of oil in the subcontinent for centuries. To improve quality and quantity of Brassica spp., the presence of sufficient genetic diversity is very important for quality, quantity and utilization of oil in human nutrition. Improvements in molecular marker technology offer a potential tool for the efficiency and affordability of variety testing. In the present study, quality of seed was tested using different qualitative tests. Biochemical markers namely isozymes (Peroxidase and Esterase), SDS-PAGE protein and molecular markers viz., SSR, ISSR were used to study the polymorphism amongst different cultivars of Indian mustard. The moisture percentage of all cultivars was found in the ranged of 3.93-10.02%. Oil content in genotype SKM 9033 showed highest oil and RAYAD- 9602 showed lowest oil. Protein content and true protein in mustard cuhivars ranged from 27.90-33.69% and 10.75-20.01%, respectively. Sinigrin content in mustard cultivars found in the range of 2.49-46.80 nmole/g. Genotype Bio-Q-44-279 had the highest sinigrin concentration 46.80 nmole/g and SKM-9033 had the lowest sinigrin concentration 2.49 ^mole/g. Isozyme pattern of peroxidase and esterase generated significant correlation amongst the clusters. Electrophoresis of seed protein showed a total 28 bands in mustard genotypes and the intensity of the bands varied among all the genotypes. Simple Sequence Repeats amplification of mustard genomic DNA using 8 primers generated 252 scorable bands with average of 31 bands per primer. Percent polymorphism ranged from 33.33% (BRMS-08) to 100%. Polymorphic information content (PIC) ranged from 0.37 (BRMS-06) to 0.83 (BRMS-07). The similarity coefficient of all mustard cuhivars ranged between 0.036 to 0.947. This result indicated existence of genetic variation occurred among different genotypes. Clustering pattern of dendrogram generated by using the pooled SSR data formed two major clusters viz. having similarity coefficient of 0.39 to 0.94. It indicated genetic variability among the different genotypes. Cophenetic correlation was found r = 0.89, reflecting very good fit of the dendrogram. Both Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) markers were efficient to assess genetic diversity within species of mustard. Dendrogram obtained from both markers showed two major clusters A and B. Cophenetic correlation were r = 0.89 for SSR and for ISSR r = 0.85, reflecting very good fit for dendrograms. SSR and ISSR showed average polymorphism of 82.91% and 96.02%, respectively. It showed that ISSR was more polymorphic than that of SSR. Cophenetic correlation of both SSR and ISSR similarity matrices were r = 0.80, suggested suitable fit for cluster analysis. From these results it could be concluded that clusters produced by SSR and ISSR were conserved and were highly correlated with each other. The resuUs showed that, ISSR was more informative than SSR in assessing genetic diversity of mustard genotypes.