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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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Subject: Animal Biotechnology × End date: 2014 × Start date: 2012 × Type: Thesis × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    Evaluation of Gender Pre-selection through Sperm Enrichment Techniques Using Real-Time PCR in Bovines
    (AAU, Anand, 2013) ROY, LATISH CHANDRA; Panchal, M. T.
    The present work was undertaken to assess the efficiency of the classical X and Y sperm enrichment methods, viz., sperm swim-up, gravity sedimentation, Sephadex filtration, and discontinuous Percoll density gradient centrifiigation, using the modem molecular technique of Real-Time PCR or qPCR in buffalo and cow bulls, in the Department of Animal Biotechnology and Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science & AH, Anand Agricultural University, Anand. Total of 12 semen ejaculates six each from buffalo and cow bull, diluted at the rate of 1:10 with Tris-fructose-yolk- glycerol (TFYG), were collected from the breeding bulls stationed at Semen Station, Amul Research and Development Association (ARDA), Ode, Anand and subjected to the different enrichment techniques. In sperm swim-up four time bound fractions were collected using a special swim-up tube. Similarly with gravity sedimentation, three fractions, with Sephadex filtration four fractions and with discontinuous Percoll density gradient centrifugation the bottom sperm pellet was collected. DNA was extracted from all the collected fractions and the control semen samples and was further used for Real-Time PCR. Four different neat semen dilutions were taken as standards for the Real-Time PCR, viz., 100.00, 75.00, 50.00 and 25.00 per cent, consisting of 50.00, 37.50, 25.00 and 12.50 per cent X-chromosome bearing sperms,' respectively, considering the theoretical ratio of 1:1 for X and Y sperms in an ejaculate. With gravity sedimentation, the X-chromosome bearing sperm percentage in the three collected fractions of semen samples, ranged from 19.96 to 43.99 (31.90±4.44), 41.10 to 46.93 (45.01±1.32) and 38.90 to 48.07 (43.67±1.40) per cent, respectively and 21.09 to 51.32 (35.22±4.00), 35.46 to 51.32 (43.26±2.19) and 34.86 to 42.31 (39.19±1.14), respectively, in buffalo and cow bulls with an overall mean Xchromosome bearing sperm percentage of 40.19±2.08 and 39.22±1.67 in buffalo and cow bull semen, respectively. In the four semen fractions obtained post-filtration through Sephadex (G-lOO) gel, X-chromosome bearing sperm percentage values ranged from 42.51 to 52.08 (46.08±1.42), 42.75 to 52.51 (47.69±1.68), 41.43 to 50.20 (45.96±1.22) and 42.36 to 49.17 per cent (44.80±0.98) as compared to control semen samples having the range of 46.76 to 50.20 per cent (48.48±0.4) in buffalo bull and 27.82 to 48.00 (41.99±3.00), 33.02 to 52.59 (43.75±2.67), 35.09 to 52.79 (43.48±2.35) and 33.32 to 50.15 (42.50±2.20) per cent, compared to the control with the range of 45.94 to 50.11 (47.67±0.76) in the cow bull semen samples. The overall mean was obtained to be 46.13±0.67 and 42.93±1.21 per cent, in buffalo and cow bulls, respectively. The X-chromosome bearing sperm percentage in the three swim-up fractions retrieved ranged from 42.00 to 59.57 (48.62±2.60), 42.16 to 50.61 (45.91±1.29) and 30.13 to 51.93 (44.09±3.15) per cent, respectively, and 33.60 to 45.84 (42.30±1.80), 34.61 to 47.28 (42.86±1.84) and 31.23 to 62.58 (44.07±4.17) per cent, respectively, with an overall values of 46.21± 1.41 and 43.08±1.54, in buffalo and cow bulls, respectively. For control buffalo and cow bull semen samples the X-chromosome bearing sperm values ranged from 46.76 to 50.20 (48.48±0.48) and 45.94 to 50.11 (47.67±0.76) per cent, respectively. With discontinuous Percoll gradient centrifugation, the X-chromosome bearing sperm percentage obtained in the bottom pellet ranged from 43.79 to 51.83 (48.93±1.93) percent against the control value of 45.07 per cent in buffalo bulls and 48.20 to 56.89 (52.42±1.23) per cent, compared to the 50.56 per cent of the control, in cow bull semen samples. No detrimental effect was observed on individual motility of the sperms following any of the sperm enrichment procedures. None of the four methods evaluated proved efficient enough in altering the sex ratio of the sperms. No significant differences in X-chromosome bearing sperms were observed in any of the methods as compared to control, except in gravity sedimentation, where, a highly significant difference was found between the different fractions, both in buffalo and cow bulls.