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Anand Agricultural University, Anand

Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.

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  • ThesisItemOpen Access
    “DETECTION OF nifH GENE, PRELIMINARY CHARACTERIZATION BY ARDRA AND PROTEIN PROFILING OF NATIVE DIAZOTROPHS AZOTOBACTER, ACETOBACTER AND AZOSPIRILLUM”
    (Anand Agricultural University, Anand, 2012) PRAJAPATI RONAKKUMAR RAMESHBHAI; Dr. R. V. Vyas
    The native diazotrophic bacterial isolates (ABA-1, ABA- 10, ABA-14, ABA-2010, ACG-2 and ASA-1) and four reference strains (MTCC-446 and MTCC-124 (Azotobacter chroococcum), MTCC-1226 (Acetobacter diazotrophicus) and MTCC-2306(Azospirillum lipofarum) were used for the study. Bacterial culture were revived on their specific nitrogen free medium. All diazotrophic bacteria were characterized by Amplified ribosomal DNA restriction analysis (ARDRA), detection of nifH gene by polymerase chain reaction (PCR) and whole cell protein profiling by SDS-PAGE. Phylogenetic analysis of 16S rDNA indicated that these diazotrophic bacterial isolates belonged to respective genus Azotobacter, Acetobacter and Azospirillum and closely related to standard strains. Whole cell protein profiling by SDS-PAGE showed the specific polypeptide band of Fe protein (60 kDa) responsible for nitrogenase enzyme in all native nitrogen fixing bacterial isolates as well as standard cultures viz., (MTCC-446 and 124), (MTCC-1226) and (MTCC-2306) at three