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Anand Agricultural University, Anand
Anand Agricultural University (AAU) was established in 2004 at Anand with the support of the Government of Gujarat, Act No.(Guj 5 of 2004) dated April 29, 2004. Caved out of the erstwhile Gujarat Agricultural University (GAU), the dream institution of Sardar Vallabhbhai Patel and Dr. K. M. Munshi, the AAU was set up to provide support to the farming community in three facets namely education, research and extension activities in Agriculture, Horticulture Engineering, product Processing and Home Science. At present there seven Colleges, seventeen Research Centers and six Extension Education Institute working in nine districts of Gujarat namely Ahmedabad, Anand, Dahod, Kheda, Panchmahal, Vadodara, Mahisagar, Botad and Chhotaudepur
AAU's activities have expanded to span newer commodity sectors such as soil health card, bio-diesel, medicinal plants apart from the mandatory ones like rice, maize, tobacco, vegetable crops, fruit crops, forage crops, animal breeding, nutrition and dairy products etc. the core of AAU's operating philosophy however, continues to create the partnership between the rural people and committed academic as the basic for sustainable rural development. In pursuing its various programmes AAU's overall mission is to promote sustainable growth and economic independence in rural society. AAU aims to do this through education, research and extension education. Thus, AAU works towards the empowerment of the farmers.
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ThesisItem Open Access PHYSIO-BIOCHEMICAL ANALYSIS OF TOMATO cv. GAT 5 FRUITS FOR SHELF LIFE IN RESPONSE TO EXOGENEOUS MELATONIN(Department of Biotechnology B. A. College of Agriculture, Anand Agricultural University Anand, 2020) Tushar Ranchhodbhai Gadhavi; Dr. J. J. DhruvThe present investigation was undertaken on tomato fruit (Solanum lycopersicumL.) in order to estimate biochemical characterization and molecular analysis. The tomato fruits and genotypes were received from Main vegetable research centre (MVRS), Anand Agricultural University, Anand during summer 2019-20. The main objective of the experiment was to study the Physio-biochemical property of tomato fruit one variety and characterization of ten tomato (Solanum lycopersicum L.) genotypes by molecular markers. The various physio-biochemical characters were analyzed at Biochemistry Department, A.A.U., Anand, while the molecular characterization was done at Biotechnology Department, A.A.U., Anand.ThesisItem Open Access MOLECULAR STUDIES FOR APHID [Lipaphis erysimi (Kalt.)] RESISTANCE IN ADVANCED GENERATION (F4) OF Brassica INTERSPECIFIC HYBRID GM-3 × PUSA SWARNIM(DEPARTMENT OF AGRICULTURAL BIOTECHNOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2019) Desai Pallavi Kumari Onkarnath; Dr. Sasidharan. NRapeseed mustard is the third important oilseed crop in the world after soybean (Glycine max) and palm (Elaeis guineensis Jacq.) and globally, India account for 72 lakh hectare, 85.0 lakh tonnes (lt) and 1184 Kg per hectare of the total acreage , production and productivity respectively. Whereas Gujarat account for 1.90 lakh hectare , 3.06 lakh tonnes (lt) and 1611 Kg per hectare of the total acreage, production and productivity respectively. The mustard crop suffers from various biotic and abiotic stresses. Among the different pests and diseases affecting this crop, mustard aphid, Lipaphis erysimi (Kalt.) is the major limiting factor for qualitative as well as quantitative production of mustard, which infests the crops right from vegetative stage to pod stage and accounts for 27% to 69% of yield loss with 15% reduction in its oil content and 75% productivity losses. In this context, breeding for aphid resistance and identification of molecular markers for this trait could be a valuable approach for development of resistant genotype.ThesisItem Open Access IN SILICO IDENTIFICATION OF microRNAs AND THEIR VALIDATION IN PEARL MILLET [Pennisetum glaucum L.](AAU, Anand, 2016) AMIT KUMAR; Dr. R. S. FougatThe endogenous small non-coding functional microRNAs (miRNAs) are short in size, ranging from ~21 to 24 nucleotides in length and play a pivotal role in gene expression in plants and animals by negative regulation or silencing of genes either by cleavage or blocking of translation of homologous mRNA. Although various highthroughput and expensive techniques like forward genetics and direct cloning are employed to detect miRNAs in plants but comparative genomics complemented with novel bioinformatic tools paced the way for efficient and cost-effective identification of miRNAs through homologous sequence search with previously known miRNAs. Many miRNAs have been identified and investigated extensively in plant species with available sequences in NCBI database. However, only one miRNA has been identified in pearl millet and reported on PMRD. Peal millet is widely grown crop in arid and semi-arid regions of world hence this crop has great importance in dry land agriculture. In this study, an attempt was made to identify and characterize conserved miRNAs in pearl millet expressed sequence tag (EST) sequences; genome survey sequences (GSS) and nucleotide sequences using bioinformatics approach. For ii identification of novel miRNAs in pearl millet, a total 7240 known mature miRNAs of plant kingdom were searched for homology against 6014 unigene sequences resulting in identification of 14 potential miRNA candidates belonging to 11 different families. The majority of predicted pearl millet miRNAs were 21 nucleotides in length and they were found on both the 5′ and 3′ arms of the stem-loop hairpin secondary structure. The pearl millet pre-miRNAs varied in length from 60 to 109 nt. Five out of 14 predicted miRNAs were confirmed in young plants of pearl millet using quantitative real time polymerase chain reaction (qRT-PCR). The psRNATarget server predicts 34 potential target genes and their probable functions. Most of the pearl millet miRNA target gene involved in stress response, transcriptional regulation, signal transduction, DNA replication proteins, male sterility, metabolic enzymes, plant growth and development. The results of the present study will shed more light on the understanding of molecular mechanisms of miRNA in pearl millet which may aid in development of novel and precise techniques to understand some post-transcriptional gene silencing mechanism in response to stress toleranceThesisItem Open Access GENDER IDENTIFICATION USING LOOP MEDIATED ISOTHERMAL AMPLIFICATION (LAMP) IN PAPAYA (Carica papaya L.) var. Madhubindu(AAU, Anand, 2016) RATHOD DIVYARAJSINH VIRENDRASINH; Dr. Subhash N.Experiments were conducted to screen and validate suitable male specific markers in the identification of sex in papaya (cv. Madhubindu). From the reported markers W11, T12, Napf and CPSM 90 showed detectable results in sex determination and was used for further experimentation with routinely used PCR method as well as loop mediated isothermal amplification (LAMP). Using screened markers unknown samples were identified with 100% efficiency of the marker used in predicting the sex type. At the time of flowering all the predicted males as well as female were confirmed visually. Moreover, after the detection with PCR, identification of papaya DNA samples were carried out with turbidity method, fluorescence method (Calcein, SYBR green, HNB, EtBr dyes). Using four primers i.e. W11, T12, Napf and CPSM90, LAMP reaction was performed initially with confirmed male and female DNA samples. Among the various dyes used, detection with calcein dye was most revealing and intense fluorescence colour was obtained. Among various primers used T12 primer was found most prominent. With the use of T12 primer and calcein detection system, various experiments were conducted for optimization of reaction time, temperature, starting quantity of DNA and concentration of enzyme. Thus in this study 4 ii male specific markers were developed for sex identification using both PCR and loop mediated isothermal amplification (LAMP). LAMP reaction carried out by T12 primer using calcein dye showed promising results in terms of rapid reaction time (< 1h), isothermal condition (55°c), a high efficiency (20 ng of DNA required in reaction mixture) and concentration of enzyme (8 units of the enzyme).ThesisItem Open Access “EFFECT OF LIMITED IRRIGATION ON QUALITY OF DURUM WHEAT (Triticum durum Desf.)”(Anand Agricultural University, Anand, 2016) Arpita Jayeshbhai Dalwadi; Dr. J. G. TalatiDurum wheat (Triticum durum Desf.) is the only tetraploid species of wheat which is cultivated worldwide. Durum wheat contains high protein, as well as its strength and structures and interactions of the grain storage proteins gliadin and glutenin make durum good for special uses, the most well-known being pasta, spaghetti and macaroni, extensively in bread making.ThesisItem Open Access “MORPHOLOGICAL, BIOCHEMICAL AND MOLECULAR DIVERSITY ANALYSIS IN FODDER MAIZE (Zea mays L.)”(Anand Agricultural University, Anand, 2016) Mohammedtarik Mahiyuddin Saiyad; Dr. Sushil KumarAn investigation was carried out at Department of Biotechnology, Main Forage Research Station and Department of Biochemistry, B. A College of Agriculture, A. A. U., Anand to study morphological, biochemical and molecular diversity analysis in fodder maize (Zea mays L.) genotypes procured from different sources. A set of twenty one polymorphic SSR primers out of 80, were used to evaluate the genetic diversity among genotypes. A total of 133 alleles, ranging from 2 (umc1060, umc1623) to 14 (dupssr34) were detected with a mean of 6.33 allele per locus. The dissimilarity matrix, generate dendograms with the unweighted Neighbor- Joining (NJ). NJ clustering grouped 75 genotypes into three main clusters A, B and C with 28, 28 And 19 genotypes, respectivelyThesisItem Open Access “GENE EXPRESSION STUDIES DURING SOMATIC EMBRYOGENESIS IN DATE PALM (Phoenix dactylifera L.) var. Barhee(Anand Agricultural University, Anand, 2016) A J DHANYA; G. B. PatilDate palm (Phoenix dactylifera L., 2n=36) is an ancient important dioecious fruit tree of semi-arid and arid regions of the world. It is one of the nourishing natural foods available to human that consist of 70% carbohydrates (mostly sugars) and 15 to 30% water. The flesh of dates contains 60 to 65% sugar, about 2.5% fiber, 2% protein and less than 2% of each fat, minerals, and pectin substances (Okereet al., 2011). It is rich in iron, potassium, calcium, and with very low sodium contentThesisItem Open Access “CHARACTERIZATION OF OKRA (Abelmoschus esculentus L. Moench) GENOTYPES THROUGH BIOCHEMICAL AND MOLECULAR MARKERS”(Anand Agricultural University, Anand, 2016) KINJAL HARIBHAI BHUTAKA; Dr. J. J. DhruveOkra (Abelmoschus esculentus L. Moench) is known in many englishspeaking countries as lady's fingers is a flowering plant in the Malvaceae family. Okra is cultivated for its fibrous fruits or pods containing round seeds. The fruits are harvested when immature, and it can be cooked in a variety of ways. Morphological attributes like plant height, fruit length, fruit girth, fruit volume and fruit density were measured, as well as various phytochemicals from seed viz., flavanoids, glycosides, saponins, tannins, alkaloids were studied and it resulted flavanoids, glycosides, saponins are present but tannins and alkaloids are absentThesisItem Open Access “GENETIC DIVERSITY ANALYSIS AND AMPLIFICATION OF SSR MARKERS ASSOCIATED WITH WBPH (Sogatella furcifera, Horvath) RESISTANCE IN RICE (Oryza sativa L.)”(Anand Agricultural University, Anand, 2010) NIKAM VIVEK SUDHAKAR; Dr. G. C. JadejaRice (Oryza sativa L.) is the most important food crop in the world and a model cereal species for molecular researches. Rice is the staple food in many parts of the world, including many developing countries in Asia, Africa, and Latin America. Through genetic studies, eight major genes conferring resistance to WBPH in rice germplasm Wbph 1 to Wbph 8 have been reported. Among these, wbph 4 is recessive, whereas, the other seven are dominant genes. Besides major genes, QTLs have been reported for WBPH resistance
