Thumbnail Image

Theses (PG)

Browse

2018 - 20194
Reset filters
Subject: Veterinary Parasitology × End date: 2021 × Thesis Type: M.V.Sc. ×
Reset filters

Search Results

Now showing 1 - 4 of 4
  • Thumbnail Image
    ThesisItemOpen Access
    MOLECULAR CHARACTERISATION AND IDENTIFICATION OF IMMUNOREACTIVE POLYPEPTIDES OF FILARID NEMATODES OF DOGS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCIES UNIVERSITY, BIDAR, 2019) ARAVINDA, K.; ANANDA, K. J.
    A study on the molecular characterization and identification of immunoreactive polypeptides of filarid nematodes of dogs in Mangalore, Karnataka state was conducted. Different methods were used for detection of microfilariosis in dogs. A total of 250 dogs screened during the present study, 62 % harboured microfilaria. In the present study, three methods used for detection of microfilariosis in dogs. Modified knott’s method detected 94.19 % of the positive samples, direct wet smear and giemsa’s stained blood smear examination detected 61.93 and 57.42 % positive samples. Morphological studies of microfilaria were done by using giemsa’s and methylene blue phosphate staining techniques revealed microfilariae were unsheathed with blunt head and a tapering tail and cuticle appeared striated. The length and width of microfilaria when measured by giemsa’s and modified knott’s technique were 292.2 ± 1.267 μm, 334.9 ± 4.573 μm, 10.65 ± 0.068 μm and 6.862 ± 0.133 μm respectively. The histochemical staining technique revealed acid phosphatase activity at anal pore region of microfilaria by showing red azo dye indicating the microfilaria were of D. repens. Confirmatory of identification of species was done by PCR using species specific primers showing amplification of mitochondrial cytochrome oxidase subunit 1 (COI) gene with single amplicon 350 bp indicated D. repens. The protein profile of microfilarial and adult worm antigen of D. repens was evaluated using SDS-PAGE. The protein profile of microfilarial antigen showed six polypeptides ranging between 183 kDa to 29 kDa. A total of thirteen polypeptides were identified in adult worm antigen ranging from 208 kDa to 29 kDa. The immunoreactive polypeptides detected on western blot with microfilarial and adult worm antigen of D. repens using known positive serum with anti-dog IgG conjugate at 1:1000 dilution revealed 50 kDa and 29 kDa. Key words: Molecular characterization, Immunoreactive polypeptides, filarid nematodes, dog, Mangalore.
  • Thumbnail Image
    ThesisItemOpen Access
    EVALUATION OF LARVAL EXTRACTS OF CHRYSOMYA MAGGOTS FOR ANTIBACTERIAL PROPERTY
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-08) GEETHA, N.; Dr. PLACID E DʼSOUZA
    In the present study, the larval extracts of Chrysomya maggots was tested for antibacterial property. The maggots were collected from wounds and carcasses in and around Bengaluru, India. The maggots from wounds were morphologically identified as C. bezziana and of carcasses were C. megacephala and C. albiceps. For larval extracts, the larvae were collected from the fly colony of C. megacephala which was successfully maintained in the laboratory, whereas larvae of C. bezziana collected from the fresh wounds due to their obligation of oviposition on fresh wounds. About two hundred, third instar larvae of Chrysomya specie
  • Thumbnail Image
    ThesisItemOpen Access
    PREVALENCE AND MOLECULAR DETECTION OF BABESIA SPECIES IN SHEEP AND GOATS
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) SHAKUNTALA KAGE; Dr. G. S. MAMATHA
  • Thumbnail Image
    ThesisItemOpen Access
    EVALUATION OF LARVAL EXTRACTS OF CHRYSOMYA MAGGOTS FOR ANTIBACTERIAL PROPERTY
    (KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR, 2018-07) GEETHA, N.; Dr. PLACID E DʼSOUZA
    In the present study, the larval extracts of Chrysomya maggots was tested for antibacterial property. The maggots were collected from wounds and carcasses in and around Bengaluru, India. The maggots from wounds were morphologically identified as C. bezziana and of carcasses were C. megacephala and C. albiceps. For larval extracts, the larvae were collected from the fly colony of C. megacephala which was successfully maintained in the laboratory, whereas larvae of C. bezziana collected from the fresh wounds due to their obligation of oviposition on fresh wounds. About two hundred, third instar larvae of Chrysomya species were incubated in 2 ml of ultra-pure distilled water for one hour, centrifuged to 10,000X g and supernatant was used as excretions/ secretions (ES). The ES and whole larval extract of C. megacephala and ES of C. bezziana larvae of third instars were tested for antimicrobial property against Staphylococcus aureus (MRSA and MSSA), Escherichia coli, and Pseudomonas aeruginosa by agar well diffusion method, disc diffusion method and Minimum inhibitory concentration. The ES of C. bezziana was not effective. Contrastingly, the ES of C. megacephala showed zone of inhibition at the lowest concentration of 0.23 µg by Agar well diffusion method except for P. aeruginosa but ES was found effective by disc diffusion method against all the three bacteria. The minimum inhibitory concentration was 1.35 mg for S. aureus. Whereas the whole larval homogenate failed to show the antimicrobial property.