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Open Access
Morphological variations in seed and seedling characteristics of different seed sources of Acacia nilotica (L.)Willd. ex Delile
(Department of Forest Genetic Resources, OUAT, Bhubaneswar, 2016-08-01) S, Ajay Kumar; Hossain, M M
The present investigation entitled, “Morphological variations in seed and seedling characteristics of different seed sources of Acacia nilotica (Linn.) Willd. Ex Delile” was carried out in the Department of Forest Genetic Resources, College of Forestry, Orissa University of Agriculture and Technology, Bhubaneswar. The experiment was laid by CRD design with 3 treatments, there were significant variations found in seed characters except moisture content. The seed length and seed width were found to be maximum in seed source in GUJ S-3 (8.11mm and 6.77 mm, respectively)whereas minimum was recorded in MH RAN-46 (6.12 and 5.25 mm, respectively).The maximum seed thickness, weight and moisture content of 4.84 mm,23.06g and 9.97%, respectively werefound inGUJ S-1. Weight of seeds ranged from 10.28-23.06gm in MH RAN-46 and GUJ S-1 respectively. Genetic variation studies found that seed length had maximumPCV (33.13 %), GCV (32.87 %), and genetic gain (54.19 %) among all the seed characters studied. All the seed parameters are found to be phenotypically and genotypically positively correlated with each other.The Germination parameters were found to be significantly different from each other among seed sources. Germination percentage (80%), mean daily germination (2.88%) and mean germination time (16.77 days) were found maximum in seed source, GUJ S-1 whereas rest of the parameters like Germination energy (55.11%), peak value (3.5), Germination value (9.66), and Germination speed (4.24) were found to be maximum in the seed sources, GUJ S-2. The seed source MH RAN-46 recorded minimum for all germination traits. For germination parameters range of genetic variation is heritability (40.92-94.86%), PCV (61.29- 11.52%), GCV (56.06-11.22%) and genetic advance (105.64-28.06 %).The seedling traits showed significant variation among different seed sources. The seed source GUJ S-1 was found to be superior in all the growth parameters over other seed sources except MH RAN-43 in height. Seed source GUJ S-1 recorded maximum values in collar diameter (8.43 mm), number of primary branches (4.5 nos.), dry seedling weight (25.50 g), dry root weight(6.57g), seedling vigour index (11203.59), seedling volume index (6610.19), and seedling quality index (2.15) in eight months. The tallest seedling (115.33 cm) was obtained in the seed source MH RAN-43 and the longest root length (59.23 cm) was found in MH RAN-48. Most of the growth traits are found to have high heritability. The PCV, GCV of variation and genetic advance was maximum in volume index i.e., 45.74%, 42.26 % and 80.44%, respectively. Shoot fresh weight was significantly and positively correlated with collar diameter (0.863), number of primary branches (0.954), shoot dry weight (0.961), root fresh weight (0.679), quality index (0.851), vigour index (0.663) and volume index (0.761).
Open Access
Management of soil borne pathogens of cowpea (Vigna unguiculata L.) and its effect on nodulation
(Department of Plant Pathology, OUAT, Bhubaneswar, 2021) Bhavani, Kukkala Manga; Dhal, Arabinda; Senapati, Akshaya Kumar; Panda, Narayan
The investigation entitled “Management of soil borne pathogens of cowpea (Vigna unguiculata L.) and its effect on nodulation.” were carried out in the Department of Plant Pathology, College of Agriculture and AICRP on Forage Crops and Utilization, OUAT, Bhubaneswar. The symptoms of stem rot were observed on collar region, leaves and stems as yellowing and subsequent wilting of whole plant. Isolation of test fungus showed the presence of Sclerotium rolfsii Sacc. and identified on the basis of morphological characters. The combination of mixed fungicides viz., carbendazim 12% + mancozeb 63% at 0.2%, tebuconazole 10% + sulphur 65% at 0.1% and triazole group of fungicides viz., propiconazole 25%, tebuconazole 25.9%, hexaconazole 5% except tricyclazole 75% recorded inhibition of Sclerotium rolfsii growth by 100% followed by chlorothalonil 75% at 0.2% (83.34%), copper oxy chloride 50% at 0.2% (72%) and tricyclazole 75% at 0.1% concentration (66.41%) and least by carbendazim 50% at 0.15% (2.18%).The antagonist Trichoderma harzianum is most efficacious with 72.97% mycelial growth inhibition followed by Trichoderma viride with 67.89% mycelial growth inhibition. The bacterial antagonist Pseudomonas fluorescens has 59.47%mycelial inhibition. The chemical seed treatment with tebuconazole 2DS @ 1g/kg seed along with basal soil application of T. viride @ 4kg /ha enriched in FYM @ 250 kg/ha was found the best treatment with 94.2% germination which reduced the diseased by 62.1% and increased the yield by 16.6% compared to control. Among nonchemical treatments, seed treatment with T. viride along with basal application of the same bioagent was found superior recording 89.4% germination followed by P. fluorescens (87.6%). The T. viride treatment reduced the disease by 49.9% and increased the yield by 13.4% when compared to control. When seeds treated with chitosan @ 0.5% recorded 83% germination, 33.2% disease reduction and 11.6% increase in yield over control. The seed treatment with neem seed extract @ 5% resulted in 82.5 % germination, 41 % reduction in disease with 10.5 % increase in yield. It is evident that a few seed treating chemicals under test have somewhat inhibitory effect on nodulation whereas mostly have a positive effect on nodulation; or else the nodulation is not affected by fungicides and biocontrol agents. At early stage of nodulation (30 DAS) control pots exhibited highest nodulation (27.7) which is at par with seed treatment with metalaxyl 4% + mancozeb 64%, carbendazim 50%, carbendazim 12% + mancozeb 63% and Trichoderma viride 1%. However significantly less nodulation was recorded in Tebuconazole combinations either with trifloxystrobin 25% or Sulphur 65% and with Thiram 75% treatment. However, in active nodulation stage i.e., at 45 DAS, Metalaxyl 4% + mancozeb 64% recorded the highest nodulation (36.7) which is statistically at par with carbendazim 50% (31.7) and carbendazim 12% +mancozeb 63% (33.3). Hence most of the seed treating chemicals are not interfering with native rhizobial activity and rather improves nodulation activity in cowpea.
Open Access
Leaf Blight of Anthurium (Anthurium andraeanum) and its managemen
(Department of Plant Pathology, OUAT, Bhubaneswar, 2021) Mohapatra, Deepali; Mahapatra, Shyama Sundar; Senapati, Akshaya Kumar; Beura, Sashikala
Anthurium ranks ninth in the global flower trade and commands a fair price for both cut flowers and whole plants. Anthurium is the latest sensation of Indian floriculture scene and gains its fame and respected status by exhibiting its striking ensemble, which is created unitedly by its spadix and its spathe, within the economically essential ornamentals, allowing its use in interior and exterior decoration and also to its use as a cut flower. As in the most other cultivated plants, Anthurium plant too is subjected to attack by various pathogens during its growth period. Among all the diseases, leaf blight disease caused by Helminthosporium sp. and Colletotrichum gloeosporioides (Mancini et aI.,1973) is newly emerging threat to all the Anthurium growers of Odisha. Therefore, the research work was undertaken on the title ―Leaf blight of Anthurium (Anthurium andraeanum) and its management‖. The disease was first reported during 2020 from the gardens of Department of Floriculture and Landscaping, College of Agriculture, OUAT, Bhubaneswar. According to the symptomatology study, the typical brown spots began as a water-soaked lesion on the upper surface of the leaves, which usually developed from the tip or along the margin of the upper surface of the leaf. The size of these spots grew larger with age, until two or more spots merged and formed elongated reddish brown necrotic patches surrounded by a yellow halo. The centres of such spots eventually turn greyish white, with a small brown zone surrounding them. The pathogenicity of the isolated fungi Helminthosporium sp. and Colletotrichum sp. was tested on potted Anthurium plants and re-isolated fungi were found identical to the original isolated one. The growth of the fungus Helminthosporium sp. was best on Potato Dextrose Agar medium (68.20 mm) followed by Potato Carrot Infusion Agar medium (66.80mm) and the best medium for growth for Colletotrichum sp.was Potato Dextrose Agar medium (76.88 mm) followed by Carrot Agar medium (74.24mm). In vitro evaluation of fungicides against Helminthosporium sp. recorded maximum inhibition (100%) by Tebuconazole (0.1%), Tebuconazole 10% + Sulphur 65% (0.1%) and Tebuconazole 50% + Trifloxystrobin 25% (0.1%) and against Colletotrichum sp. maximum inhibition was recorded by Tebuconazole (0.1%), Difenoconazole (0.1%), Tebuconazole 10% + Sulphur 65% (0.1%) and Tebuconazole 50% + Trifloxystrobin 25% (0.1%). In vitro efficacy of different bio control agents revealed that, Pseudomonas fluorescens was most effective in inhibiting the growth of Helminthosporium sp. (54.44%) and Trichoderma viride was most effective against Colletotrichum sp. (58.88%). Further, management of the disease under in vivo condition is suggested in subsequent years to draw a conclusion.
Open Access
Management of ripe fruit Rot disease in Capsicum (Capsicum annum L)
(Department of Plant Pathology, OUAT, Bhubaneswar, 2021) Sahoo, Chinmaya Kumar; Sarkar, Subrata; Senapati, A .K.; Mohanty, Anita
Capsicum (Capsicum annum L) commonly known as ‘sweet pepper’ or ‘Shimla mirch’ is native of Mexico with secondary centre of origin in Guatemala is an important and nutritious vegetable crop belongs to Family Solanaceae (Bukasov, 1930) which was introduced to Shimla hills (India) by the Britishers in the 19th century and commercially grown in Himachal Pradesh, Jammu and Kashmir, Uttarakhand, Arunachal Pradesh and Darjeeling district of West Bengal. In India, area under bell pepper cultivation is 22,000 hectares with 2.68 lakh tonnes production and productivity of 12.76 tonnes per hectare .(Annon., 2016). In Odisha it is cultivated in Mayurbhanj ,khorda , Sundergarh Odisha data was reported at 6.770 Ton th in 2017 (Anonymous 2017). Capsicum crop suffers from number of diseases under Indian condition. These include damping off (Pythium aphanidermatum and Phytophthora spp.),leafspots (Cercospora capsici and Alternariasolani), anthracnose and ripe rot (Colletotrichum capsica), fruit rot and leaf blight (Phytophthora spp.), powdery mildew (Erysiphe cichoracearum and Leveillula taurica), early blight (A. solani), wilt (Fusarium oxysporum), frog eye rot (Phaeoramularia capsicicola),leafspot (Septoria lycopersici), fruit spot(Phoma spp.), stem rot (Macrophomina phaseoli), dry rot (Sclerotium rolfsii) and fruit rot(Phomopsis spp.) as described by different workers (Chadha, K.L,2003., Gupta, V.Kand Paul , Y.S, 2003 and Gupta , S.K. and Thind, T.S, 2006 ) . Among them ripe fruit rot of capsicum is an important one since, the disease is prevalent in all the capsicum growing areas of India as well as in Odisha and considered as major hindrance for cultivation . The present study was, therefore, undertaken in the present dissertation and the following results were obtained in the course of investigation. use of fungicide is most effective management practice of any pathogen. Morphological characterization of the pathogen revealed that the fungus produced hyaline, septate, grey to olive light pink mycelia were hyaline. Conidiophores are unbranched . Conidia are hyaline, sickle shaped, nonseptate with pointed end and oil globules. Acervuli are black dot like pin head size with setae. In vitro efficacy of 7 sole compound against C. capsici showed that Difenoconazole recorded 100.0% mycelia inhibition in all the tested concentrations followed by Tebuconazole recorded complete inhibition (100.0%) at a concentration of 0.2% to onwards. However However, fungicides namely, , Azoxystrobin, Trifloxystrobin recorded cent percent inhibition at a concentration of 0.25% and 0.3% respectively . While Copper hydroxide and copper oxychloride fungicides recorded an increased inhibition with increasing concentration . Among the 3 combination compound chemicals tested against the test pathogen under in vitro condition showed that Tebuconazole 50% + Trifloxystrobin 25% WG and Azoxystrobin 11%+Tebuconazole 18.3% SC recorded complete inhibition (100.0%) at 0.25 %concentrations on wards, However, Tebuconazole 10% + Sulphur 65% WG showed complete inhibition(100%) at 0.3%. Among the different bio-control agents , Trichoderma viride was recorded maximum inhibition of mycelial growth (75.35%). However, the least inhibition was observed in Bacillus subtilis (57.75%). In vitro evaluation of plant extracts revealed that leaf extracts of neem was recorded maximum inhibition of mycelia growth (75.65%) at 20% concentration. However, least inhibition was recorded in lantana leaf extract( 24.45%).
Open Access
Microbes responsible for post harvest spoilage of apple and their characterization
(Department of Plant Pathology, OUAT, Bhubaneswar, 2021) Subudhi, Debasmita; Mahapatra, Shyama Sundar; Senapati, Akshaya Kumar; Nayak, Umashankar
Apple (Malus domestica Borkh.) or (Malus pumila) is commercially the most important temperate fruit and is fourth among the most widely produced fruits in the world after banana, orange and grape. China is the largest apple producing country in the world. India ranks five in the world with the apple cultivation in area of about 301 thousand hectare with total annual production of 2370 thousand metric tonnes. The present study was conducted to develop methods of post harvest management of apple fungal decays to increase its shelf life in odisha condition. Three different types of post harvest fungal pathogens were found associated with rotting of apple fruits. Blue mould rot due to P. expansum was found to cause 50% of post harvest rot of apple, though, Aspergillus niger and Aspergillus flavus were also causing rots. All the three fungi were isolated and identified based on their morphological characters. Maximum growth and sporulation of the fungi Aspergillus niger and Aspergillus flavus were observed on Potato Dextrose Agar medium followed by Czapek’s Dox Agar medium and Malt extract agar medium. Maximum radial growth and sporulation of the fungus Penicillium expansum was observed on Czapex Dox Agar medium and Potato Dextrose Agar medium followed by Malt extract agar medium. Six sole compoud fungicides and two combination products were tested in-vitro against Aspergillus niger, Aspergillus flavus and Penicillium expansum using the poisoned food technique. Among the six tested sole compound chemicals against Aspergillus niger and Aspergillus flavus , Tebuconazole and Difenoconazole recorded 100.0% mycelia inhibition in all the tested concentrations Cent percent inhibition of these two fungi was observed at 0.1% concentration of Tebuconazole 18.3%SC + Azoxystrobin11%. In case of Penicillium expansum, Tebuconazole and Difenoconazole recorded 100.0% mycelia inhibition in all the tested concentrations and Tebuconazole 18.3 % SC + Azoxystrobin 11% combination was proved to be effective at 0.05% concentration and showed 100% inbition at 0.1% concentration. In vitro assay of plant extracts showed effectiveness of Ocimum extract on both the species of Aspergillus and Penicillium expansum. Biocontrol agent Pseudomonas fluorescens showed maximum inhibition of radial growth of Aspergillus niger and Aspergillus flavus (62.93 % & 61.75 %, respectively) whereas Trichoderma viride could achieve maximum inhibition of radial growth of Penicilium expansum (67.94%).
Open Access
Characterization & antifungal efficacy of nano copper(Cu) particles against early leaf blight disease of tomato
(Department of Plant Pathology, OUAT, Bhubaneswar, 2021) Shukla, Anju; Mishra, Mihira Kumara; Senapati, Akshaya Kumar; Dwibedi, Sanat Kumar
Tomato (Solanum lycopersicum L.) belonging to family Solanaceae is an important horticultural crop cultivated throughout the world. It is rich source of vitamins like A, B, C, potassium, folate, vitamin K and minerals. The lycopenes provides attractive red colour and carotene provides attractive yellow colour. Hence, tomatoes are called as “Poor man’s apple”. The crop is affected by a number of diseases of which early blight incited by Alternaria solani is an economically important disease. Considering the importance of the disease and for developing an effective management strategy, the present study was carried out in OUAT, Department of Plant Pathology, College of Agriculture, Bhubaneswar on various aspects of management including use of copper nanoparticles and fungicides against early blight pathogen A. solani. Synthesis of copper nanoparticles from three different types of plant leaves i.e. Tulsi, Neem, Bael and two chemicals methods i.e. A, B and their characterization was carried out. In vitro efficacy of copper nanoparticles against early leaf blight pathogen along with a standard fungicide were done. A new study was also carried out to know the effect of water stored in Cu container for different durations for its antifungal efficacy against A. solani. Early blight symptoms typically begin first as small, dark, circular necrotic spots. As the lesion enlarges, concentric rings with a target-like appearance surrounded by a yellow halo are evident. Thus, isolation was made from tomato plants showing typical early blight symptoms. The mycelial growth of the fungus A. solani was light greyish to black fluffy mycelial growth with circular margin. The conidia were variable in the size of 35.3 µm 12.8 μm. The isolated fungus (A. solani) satisfied Koch’s postulates on tomato plants by employing spore suspension (2 104 spores/ml) spray inoculation technique. The average particle sizes (diameter) of Cu formulations from Neem, Tulsi and Bael were 246.5, 1,019 and 427.4 nm and that from chemicals (method A, B) were 1,537 and 867.8 nm, respectively. CuNPs yielded lowest size from Neem and highest size from chemical method A. Among the seven treatments of CuNPs, neem proved to be most effective with 75.90% growth inhibition at 500ppm & 60.24% growth inhibition at 300ppm. Chemical A showed lowest growth inhibition with 62.65% at 500 ppm & 40.96% at 300 ppm. It was evident from the results that lower sized nanoparticles showed higher antifungal efficacy compared to higher sized nanoparticles at both the concentrations clearly indicating higher penetration of lowest size CuNPs in to the fungal cells inhibiting growth and cell wall synthesis of the fungus. Also carbendazim 50 WP which was used as check exhibited much lower inhibition of mycelial growth i.e. 39.75% & 59.03% at 300 and 500ppm respectively. Therefore, dose of fungicide can be very much reduced by using nano formulations of different fungicides and hence there should be increase motivation to develop cost-efficient, high-performing nanoparticles that are environment friendly and use them as alternative to hazardous chemical fungicides. Eight fungicides were screened against A. solani under in vitro condition by poisoned food technique. Among all the treatments, Kasugamycin 3% SL was found most effective in inhibiting mycelial growth (84.34%) of A. solani @ 0.2 % while lowest mycelial growth inhibition was shown by Iprodione 50% WP @0.3% (74.69%). Seven different treatments of deionised water stored in Cu container for different durations were evaluated by poisoned food technique against A. solani. Water stored for 120 hrs and 96 hrs resulted highest mycelial growth inhibition. Water boiled in Cu container exhibited no growth inhibition suggesting nil Cu particles in the content
Open Access
Microflora associated with some common millets in Odisha and their management
(Department of Plant Pathology, OUAT, Bhubaneswar, 2021) Mahapatra, Poojashree; Biswal, Dr. (Mrs.) Gayatri; Senapati, Dr. Akshaya Kumar; Jena, Dr. Satyananda
Millets are highly nutritious, non-glutinous and non-acid forming foods. Millets have many nutraceutical and health-promoting properties especially the high fibre content. Millets act as a probiotic feeding for micro-flora in our inner ecosystem. Millets hydrate our colon to keep us from being constipated. Niacin in millet can help lower cholesterol. Millets contain major and minor nutrients in good amounts along with dietary fibre. The common millets grown in India are sorghum, pearl millet, finger millet (Major millets) foxtail, little, kodo, proso and barnyard millet (minor millets). These are one of the oldest foods known to humanity. These are one of the several species of coarse cereal grasses in the family Poaceae, cultivated for their small edible seeds. Pseudo millets are so-called because they are not part of the Poaceae botanical family, to which 'true' grains belong, however they are nutritionally similar and used in similar ways to 'true' grains. Finger millet is known as the king of millets. microflora associated with finger millet and little millet were studied in the Post Graduate Laboratory of Department of Plant Pathology, College of Agriculture, O.U.A.T during 2020-2021 with their management. The seed was collected from Pulse Research Station, Berhampur, Odisha. Four varieties of two millets like finger millet (Eleusine coracana) and little millet (Panicum sumatrense L.) seeds were used. There were Arjuna, Kalua, Bhairabi, Subhra varieties are of finger millet and Kolab, OLM-217, Saura, Tarini varieties are of little millet. Aspergillus flavus, A.niger, Fusarium oxysporum were found both associated with finger millet and little millet pathogenesis was proved by treating the seeds with respective fungus. Chemicals like Mancozeb, Difenoconazole, Copper oxychloride, Copper hydroxide used as a seed treatment in both finger millet and little millet seeds were tested in vitro and in vivo. Maximum seed germination was observed in difenoconazole with minimum seed infection followed by Mancozeb. In control, maximum seed infection was 30% and germination was 40% in vitro.
Open Access
Techniques for maximization of yield and shelf life of straw mushroom, Volvariella volvacea
(Department of Plant Pathology, OUAT, Bhubaneswar, 2021) Kar, Sribidya; Pani, Bikram Keshari; Senapati, Akshaya Kumar; Mishra, Mihira Kumara
Paddy straw mushroom has already been established as a successful commercial enterprise in Odisha. However, low and inconsistent yield from paddy straw substrate, improper management of polythene cover during cropping and poor shelf life have been identified as major concerns today. Therefore, the present investigation was carried out to maximize the yield and shelf life of straw mushroom by adopting some innovative techniques. It was revealed that maximum production( 1200 g), productivity(17.1 % BE) and economic return (C:B ratio- 1: 2.9) could be achieved by supplementation of paddy straw substrate with 5 % cotton waste compared to current practice of using paddy straw alone (720 g, 10.2 % BE, C:B ratio- 1: 1.4). Allowing polythene cover on beds up to 5 days, then removing it for surface drying and restoring back till pinhead initiation sustained significantly higher mushroom production (1110 g, 15.8 % BE, C:B ratio- 1: 2.7) compared to the current practice (720 g, 10.2 % BE, C:B ratio- 1: 1.4) of covering beds continuously till pinning. Storage temperature of 150 C was sustained shelf life well up to 96 h compared to 24 h shelf life recorded in current practice of storing mushrooms at ambient temperature. Mushroom packaging in perforated brown kraft paper carry bag performed best in terms of lowest veil opening (13 %), minimum weight loss (20.4 %) and better maintenance of texture ( up to 16 h) compared to 19.6 % veil opening, 24.4 % weight loss and maintenance of texture up to 12 h recorded in current practice of packaging mushrooms in perforated polythene carry bags. Pretreatment of fruiting bodies with CaCl2 (0.5 %) maintained higher degree of colour, texture, appearance and sustained overall shelf life up to 30 h. In case of current practice of storing mushrooms without any pretreatment, the storage life was up to 18 h only.
Open Access
Symptomatology and etiology of leaf spot disease of Giloy (Tinospora cordifolia Thunb.)
(Department of Plant Pathology, OUAT, Bhubaneswar, 2021) Raju, Riya; Senapati, Akshaya Kumar; Kumar, Sandeep; Samantaray, Devi Prasad
Giloy (Tinospora cordifolia) is one of the most important medicinal plant with various anti-inflammatory and antipyretic properties. It is also recommended as one of the important prophylactic care measures against Corona (SARS-CoV-2) virus. These plants grow extensively in different states of India, including Odisha. Only few diseases have been reported in the herb in which majority are foliar. These foliar diseases cause severe damage to the medicinal herb resulting in deterioration of its phytochemical constituents and medicinal value alongwith drastic reduction in yield. Keeping in view the importance of the leaf spot diseases occurring in Giloy which adversely affects the shrub, the present investigation was carried out with a perspective to study the symptoms of leaf spot disease predominantly occurring in and around Bhubaneswar, Odisha and to detect, identify and characterise its causal organism.Samples from all the places of collection were more or less similar with little variations. The leaf spots that appeared on the plantwere circular to irregular in shape with light grey to ash centre having dark brown margins. These spots were more evident on the ventral side of leaves while the corresponding dorsal side were yellow in colour. Later, the spots coalesced to form larger blighted areas which manyatimes covered the entire leaf surface and lead to total chlorosis, drying and defoliation of leaves.The present description of symptoms of the leaf spot diseaseis regarded as a new encounter.Pathogenicity test of isolates fromleaf samples collected from different places were done and out of 15 isolates only 9 isolates produced the symptoms similar to that of original sample. Cultural and morphological characterisation of the virulent isolates weredone. On PDA culture plates, all the isolates had white fluffy aerial mycelial growth initially and greyish black mycelium later, with little variations. The microscopic studies divulged thehyphae that washyaline, multi-celled, septate, irregularly branched, and thin (2.94 µm in diameter) initially and later thickened(4.5 µm in diameter) and changed to light grey in colour. The fungus produced obclavate to muriform,beaked,golden-brownconidia with 2-3 transverse septa and several longitudinal septa borne in chains on straight, erect, septate, golden-brown conidiophores. Based on the cultural and morphological characterisation,the fungus was identified as Alternaria alternata and was further confirmed with the help of mycologists from the Department of Plant Pathology, OUAT, Bhubaneswar. No earlier worker has reported the infection of Giloy byAlternaria alternata, hence, to the best of my knowledge it is a new report. An effort was also made to determine most suitable medium for appreciable growth and sporulation of Alternaria alternata in the laboratory, using five different culture media. Among all the media used for study, the PDA was found to be the best support formycelial growth of the fungus as well as its sporulation. It was followed by Richard’s Agar in both the aspects. No earlier work reported nutritional requirement of Alternaria alternatacausing leaf spot of Giloy. The present work therefore, is bound to cut the first turf forfuture workers

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