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Author: Arati Yadawad × End date: 2013 × Start date: 2013 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    Pyramiding of Leaf Rust Resistance Genes, Lr24 and Lr28 into Bread Wheat Variety, DWR162 (Triticum aestivum L.) Through Marker Assisted Selction
    (UAS Dharwad, 2013) Arati Yadawad; R. R. Hanchinal
    The present investigation was undertaken to pyramid two effective leaf rust resistance genes Lr24 and Lr28 for genetic enhancement of a ruling variety of Peninsular Zone, DWR 162 using marker assisted selection. ‘PBW 343’ near isogenic lines with both Lr24 and Lr28 genes were used as donor for introgression of these two genes employing marker assisted backcrossing. The SCAR markers, SCS1302607 and SCS421570 linked to Lr24 and Lr28 genes facilitated the identification of individual plants possessing the targeted genes. BC1F2 and BC2F1 generation plants had high resemblance with recurrent parent ‘DWR 162’. DUS characterization revealed that six plants in BC1F2 and 132 plants in F2 were highly resembled ‘DWR 162’ with phenotypic rust resistance and better yield potential. Further, molecular characterization of these plants with SCAR markers revealed that four plants in BC1F2 and 38 plants in F2 had both Lr24 and Lr28 genes. Plants selected with both leaf rust resistance genes Lr24 and Lr28 showed enhanced leaf rust resistance without much deviation in the traits of adaptation. High genetic variability, heritability and genetic advance in F2 population of DWR 162 X PBW 343 and high frequency of transgressive segregants recorded over better parent for spike length, spikelets per spike, plant height and grain yield per plant with upper class values for yield related traits and leaf rust resistance indicated the potentiality of this population for yield and leaf rust resistance. Genetic variability studies in F3 population indicated high potentiality of this population for grain yield per plant and leaf rust resistance. Nine recombinant lines were identified in F3 that were superior in yield parameters with both leaf rust resistance genes which had high resemblance of ‘DWR 162’. These lines were also found to be superior in agronomic traits depicting the potentiality of these lines in developing genetically enhanced DWR 162 with leaf rust resistance.