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20171
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Subject: Seed Science and Production Technology × Author: Rosada, Siqueira Jovita × End date: 2018 × Start date: 2010 ×
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    ThesisItemOpen Access
    Biopriming with Seed Endophytes and its Effect on Seedling Vigour in Greengram [Vigna radiata (L.) Wilczek]
    (University of Agricultural Science, Dharwad, 2017-07) Rosada, Siqueira Jovita; Chandrashekhar S.S.
    An investigation was undertaken to assess the presence of seed endophytes in ten different genotypes of greengram viz., DGGV-2, IPM -2-14, IPM -2-14, TM -96-2, VGG, Vamban 2, China mung, Sona mung, MH-2-15 and IPM-2-03 and their ability to produce Indole-3-acetic acid and solubilize mineral insoluble phosphates and also to assess the efficacy of isolated endophytes on seedling growth in both laboratory and green house conditions. A total of 128 isolates obtained from the 10 genotypes out of which, 13 were actinobacteria and 115 were bacteria. Screening for plant growth promoting activities revealed that out of 128 isolates AUUB 417, AUUB 433, AUUB 440, AUUB 465, AUUB 507, AUUB 454 and AUUB 456 were positive for the IAA production and AUUB 456 and AUUB 507 showed positive for phosphate solubilzation. These seven isolates were used for seed treatment in all ten varieties. Among the seed treatments, variety DGGV-2 recorded higher germination (89.79 %), root length (22.46 cm), shoot length (20.56 cm), vigour index (3874), seedling fresh weight, seedling dry weight, lower electrical conductivity and moisture content compared to other varieties under both laboratory and green house conditions. Seeds treated with the isolate AUUB 456 recorded higher germination (87.90%), root length (22.31 cm), shoot length (19.12 cm), vigour index (3649), seedling fresh weight, seedling dry weight, lower electrical conductivity and moisture content as compared to seeds treated with other isolates under both laboratory and green house conditions. In the interactions of varieties and isolates (V  I) DGGV-2 with AUUB 417 and DGGV-2 with AUUB 433 showed higher vigour index in laboratory conditions and DGGV-2 with AUUB 417, DGGV-2 with AUUB 456 and IPM -2-14 with AUUB 456 showed higher seedling vigour under green house condition.