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Subject: Plant Physiology × End date: 2016 × Start date: 2014 ×
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    ThesisItemOpen Access
    Isolation, Characterization, Screening for Bioefficacy and Mass Multiplication of Fluorescent Pseudomonads
    (University of Agricultural Science, Dharwad, 2016-08) Lamani, Beeresh; Kulkarni, Shripad
    A random survey conducted during kharif 2015 in Belagavi, Dharwad and Haveri districts of northern Karnataka revealed that black rot of cabbage is a major disease in all the districts surveyed. Maximum disease index of 23.36 per cent was noticed in Belagavi district followed by Dharwad (20.17 %) and the least disease index was observed in Haveri (18.65 %). Fluorescent pseudomonads were observed in all districts surveyed and eight fluorescent pseudomonads were isolated and identified based on their morphological characters, physiological and biochemical tests. Isolates were circular and creamy white in colour. They were showed positive for oxidase, catalase, gelatin liquefaction and fluorescence tests. In vitro studies revealed that isolate KKL-FP showed maximum inhibition of Xanthomonas campestris pv. campestris by producing 18.33 mm inhibition zone. In the pot culture experiment, fluorescent pseudomonad (KKL- FP) was tested for its bioefficacy against black rot of cabbage. In seed treatment method, 10 g/kg and in seedling dip method, 20 g/ l produced maximum number of leaves and head weight per plant. Seed treatment with hot water and KKL- FP (10 g/ kg) + seedling dip (20 g/l) + 2 sprays (10 g/l) was the best treatment to reduce black rot incidence in integrated disease management. Fungicides such as carbendazim, copper hydroxide, copper oxychloride and hexaconazole were compatible at 0.1 and 0.2 per cent concentrations. Bactericides such as Bacterinashak, Bacterinol and Streptocycline were non compatible at 100, 250, 500 and 750 ppm concentrations except Bronip was compatible up to 750 ppm concentrations. The growth of fluorescent pseudomonad (KKL- FP) strain was good in King’s B broth. Temperature of 25-30 0C and 7-8 pH favoured the maximum growth and multiplication. Talc as a carrier material maintained viable cfu count (22.33 × 107 cfu/g) even up to six months of storage under normal condition.