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Subject: Plant Biochemistry × Author: Mali, Komal V. × End date: 2017 × Start date: 2012 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Enzymatic and Phytochemical Response to Spot Blotch (Bipolaris sorokiniana) Disease in Wheat
    (University of Agricultural Science, Dharwad, 2016-06) Mali, Komal V.; Mirajkar, Kiran K.
    Wheat is the important cereal crop in the world and second most important food crop of the country that contributes nearly one third of the total food grain production. Spot blotch is serious disease of wheat in warmer and humid regions of the world caused by Bipolaris sorokiniana infection. This work was conducted to evaluate the possible roles of antioxidative enzyme superoxide dismutase, catalase, glutathione reductase, phytochemical precursor enzyme phenylalanine ammonia lyase (PAL) and the pathogenesis-related proteins (PR-proteins) peroxidase (POX), total phenol content, lignin content and chlorophyll content in the resistant (NIDW295, PDW314) and susceptible (Bijaga yellow, A-9-301) durum wheat genotype in response to Bipolaris sorokiniana. 30 day after sowing inoculation was done and parameters were analysed in sample collected from uninoculated (control) and 48 hr after inoculation. Fungal inoculation induced significantly higher level of antioxidative enzyme superoxide dismutase (52.3 %), catalase (24.89 %), peroxidase (95.5 %), glutathione reductase (93.4 %) activity in resistant genotype (NIDW 295, PDW314) than susceptible genotype (Bijaga Yellow and A-9-301). Fungal inoculation induced significantly higher level of high PAL and peroxidase activity in NIDW295 and PDW314 than Bijaga Yellow and A-9-301. The total phenol and lignin content were significantly higher in resistant genotype but susceptible showed least increase. Increase in PAL and POX activity could be a determining factor for high phenolic production, lignin content and antifungal role by cell wall strengthening to enhance resistance against pathogen. Percent decrease in total chlorophyll content was higher in susceptible (Bijaga Yellow, A-9-301) than (NIDW295, PDW314). Overall study revealed that the higher amount of total phenols, lignin, antioxidant enzyme activity, phytochemical precursor enzyme PAL and chlorophyll content play an important role in defense mechanism of plants against wheat spot blotch infection in case of resistant varieties can be used as novel tool that can determined as biochemical marker for selection of resistant line against spot blotch.