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Biochemical Characterization in Cotton in Response to Incidence of Sucking Pest
(University of Agricultural Science, Dharwad, 2016-06) Divya T.C.; Vamadevaiah, H.M.
The field experiment was conducted at ARS Dharwad farm during kharif 2015-16 to study the level of biochemical metabolites namely total sugars, reducing sugars, non-reducing sugars, total phenols, condensed tannin , gossypol, available potassium, and the activity of the defensive enzymes such as nitrate reductase, peroxidase, lipoxygenase and also differential gene cationic peroxidase 3 , lipoxygenase 1 expression in resistant CPD14-1 and susceptible CPD14-2 genotypes in response to sucking pest infestation at 45, 85, 125 days after sowing. The results indicated that insect infestation increased the antioxidant enzyme activities viz., nitrate reductase, peroxidase, lipoxygenase activity and total sugars, phenols, tannin, gossypol and available potassium in resistant genotype than susceptable genotype at three different days interval. A decrease in plant height, boll number and reducing sugar was observed in the insect damage leaves compared with the control. Aphid population was also significantly higher in susceptible CPD14-2 genotype than resistant CPD14-1 genotype. The relative expression of cationic peroxidase 3 and lipoxygenase 1 gene was upregulated in CPD14-1 genotype than in CPD14-2 genotype in insect damage compared to control.