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20151
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Subject: Molecular Biology and Biotechnology × Author: YADAV SAGAR ASHOK × Start date: 1961 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    STUDIES ON PHENOLOGICAL, BIOCHEMICAL AND MOLECULAR BASIS OF ABIOTIC STRESS TOLERANCE IN COTTON
    (University of Agricultural Sciences Dharwad, 2015-06) YADAV SAGAR ASHOK; Dr. H. M. VAMADEVAIAH
    A study was conducted during 2013-14 in Agriculture Research Station, Dharwad Farm, University of Agricultural Sciences, Dharwad to validate abiotic stress tolerance in 27 genotypes of cotton on phenological, biochemical and molecular basis. Under salinity stress, reduction in seedlings fresh weight, shoot fresh weight, shoot length, shoot dry weight, root fresh weight, root length and root dry weight was significantly less in JK-4 (G11), PH 1009 (G12) and RDT-17 (G10). In genotypes JK-4 (G11) and PH 1009 (G12) significantly high percent increase in nitrate reductase and peroxidase activity was observed under salinity stress. Under moisture stress transcription factors (TF) showed alterations in the expression level. All TF genes were up-regulated in 5433 A2 A03 N83 (G16), PH 1009 (G12) and 211412 W247-85-D14-26 (G25) and while in CCH 1831 (G19) it showed down-regulation. The genotypes studied differed widely in their response to abiotic stress exhibiting that different genotypes may have different adaptation levels against abiotic stress. From phenological, biochemical and molecular studies it can be concluded that genotypes PH 1009 (G12), JK-4 (G11), 5433 A2 A03 N83 (G16) and RDT-17 (G10) are tolerant to abiotic stress and such tolerant genotypes can thus be used in introgression breeding by further confirmation.