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20151
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Subject: Molecular Biology and Biotechnology × Author: SONALI A. PARATWAGH × Start date: 1961 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    EVALUATION OF INTROGRESSION LINES FOR PRODUCTIVITY TRAITS AND RESISTANCE TO LATE LEAF SPOT AND RUST AND AN INITIATIVE TOWARDS MARKER ASSISTED BACKCROSSING IN TMV 2 IN GROUNDNUT (Arachis hypogaea L.)
    (University of Agricultural Sciences Dharwad, 2015-03) SONALI A. PARATWAGH; Dr. RAMESH S. BHAT
    Seventy eight introgression lines (ILs) from three populations (ICGS 76 × ISATGR 278-18, DH 86 × ISATGR 278-18 and DH 86 × ISATGR 5B) involving late leaf spot (LLS) and rust susceptible varieties (ICGS 76 and DH 86) and disease resistant synthetic allotetraploids (ISATGR 278-18 and ISATGR 5B) were screened for LLS and rust resistance and productivity traits during 2013 kharif. ILs differed significantly for most of the disease resistance and productivity traits. High variability, heritability and genetic advance over mean were recorded for LLS score, rust score and pod yield (kg/ha). Significant positive association was found between the occurrence of LLS and rust. Pod yield (kg/ha), pod yield per plant, number of pods per plant and test weight showed significant negative correlation with the severity of LLS and rust. Productivity traits were significantly and positively correlated with each other. Majority of the ILs showed medium pod constriction and medium pod reticulation along with slight pod beak. Two and five lines superior to ICGS 76 and DH 86, respectively selected. Of the seven, three ILs [4 (8-2), 12 (8-10) and 5 (5-7)] were also superior over GPBD 4. The selected superior lines carried resistance allele at majority of the LLS and rust resistance-linked marker loci. These ILs with high level of resistance to LLS and rust and high productivity can be considered for variety release trials or as donor in breeding programmes. An effort towards marker assisted backcross breeding (MABC) was initiated in TMV 2 for improving LLS and rust resistance by screening the markers for polymorphism. TMV2 and GPBD 4 (donor) showed polymorphism for both LLS and rust (GM2009, IPAHM103, GM1954, GM1536, GM2301 and GM2079) resistance-linked SSR markers, which can be used for foreground selection. Of the 294 background AhTE markers, 42 (14.28%) were polymorphic, which can be employed for background selection in MABC of TMV 2.