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Subject: Genetics and Plant Breeding × End date: 2003 × Start date: 2003 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    STANDARDIZATION OF IN VITRO REGENERATION PROTOCOL FOR SELECTED GENOTYPES OF SUNFLOWER (Helianthus annuus L.)
    (University of Agricultural Sciences, Dharwad, 2003) NAGHABUSHANA NAYIDU, K; GIRIRAJ, K
    An experiment was conducted in Sunflower Helianthus annuus L.) to standardize in vitro regeneration protocol for selected genotypes. The four genotypes used in the study were DSH-15B (Parental line of DSH-1), RH-857 (Restorer line of DSH-1), 6D-1 (Parental line of KBSH-1) and a popular Open pollinated variety (Morden). The various explants used were lower cotyledon, upper cotyledon, shoot tip and hypocotyls which were dissected from one week old seedlings asceptically germinated on half strength Murashige Skoog (1962) medium. Initially, Kinetin and BAP were tried alone for direct regeneration but only callus was induced. Then, at various combinations of Kinetin and IAA, different types of calli (light green, white, green compact, golden yellow coloured nodular) were induced. Among the explants, lower cotyledon was most responsive both for callus induction and shoot regeneration. The highest shoot regeneration was induced from lower cotyledon in both 6D-1 (33%) and Morden (30%) at 0.05 mg/1 IAA + 1.0 mg/1 kinetin and 0.1 mg/1 IAA +1.0 mg/1 kinetin respectively. At 0.5 mg/ 1 kinetin + 0.5 mg/1 IBA, elongation was observed in both the genotypes. About 30% of the tubes kept for elongation showed precocious flowering, which is frequently reported in sunflower. Further, of the different combinations tried for rooting, profuse rooting was induced at MS basal + lmg/1 IBA in both 6D-1 (95%) and Morden (91%) genotypes. The rooted plantlets were transferred to plastic cups for hardening. The direct regeneration was not observed in DSH 15B and RH-857 in any of the concentrations tried. However, the results observed in Morden and 6D-1 were confirmed in repeated experiments. Histological study carried out showed meristematic center amidst of the normal cells of the explant confirming direct regeneration.
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    ThesisItemOpen Access
    RELATIONSHIP OF PHENOLOGICAL TRAITS WITH PRODUCTIVITY IN CHICKPEA (Cicer arietinum L.)
    (University of Agricultural Sciences, Dharwad, 2003) SIDRAMAPPA, B; PATIL, S A
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    GENETIC VARIABILITY FOR SEEDLING VIGOUR AND ITS ASSOCIATION WITH YIELD, YIELD COMPONENTS AND DROUGHT TOLERANCE IN GREENGRAM [ Vigna radiata (L.) Wilczek]
    (University of Agricultural Sciences, Dharwad, 2003) KAVERA; SALIMATH, P M
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    MOLECULAR MARKERS, IN VITRO POLLEN RESPONSE AND THEIR ASSOCIATION WITH WILT RESISTANCE IN CHICKPEA (Cicer arietinum L.)
    (University of Agricultural Sciences, Dharwad, 2003) BRINDHA, S; RAVIKUMAR, R L
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    COMPARISON OF MATING AND SELECTION SCHEMES FOR IMPROVING PRODUCTIVITY AND ITS RELATED TRAITS IN GREENGRAM [Vigna radiata (L.) Wilczek]
    (University of Agricultural Sciences, Dharwad, 2003) KATARAKI, PRAVEEN G; KAJJIDONI, S T
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    ASSESSMENT OF GENETIC VARIABILITY IN NIGER (Guizotia Abyssinica Cass) GERMPLASM
    (University of Agricultural Sciences, Dharwad, 2003) SREEDHAR, R. V.; PRASAD
    ABSTRACT NOT AVAILABLE
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    ThesisItemOpen Access
    GENETIC STUDY ON COTTON PLANT TYPES
    (University of Agricultural Sciences, Dharwad, 2003) KANAVI, M S P; PATIL, S S
    To increase productivity of cotton there is a need for identifying plant types suitable for the situation concerned. Plant types of varieties were compared for productivity and plant type traits. These plant types were utilized in developing inter and intra plant type crosses to compare the influence of plant type diversity on heterosis for different traits. Compact varietal plant types were superior than robust because of efficient packing of bolls, better utilization of three-dimensional space and increased plant population per unit area. Intra plant type (robust x robust) crosses were developed through 8x8 diallel mating design and interplant type (robust x compact) crosses were developed through 10x3 line x tester mating design. Most of the inter and intraplant type crosses expressed significant heterosis over mid parent for the characters studied. GCA variance was found to be significant and higher in magnitude than significant SCA x'ariances for all the characters. There were potential inter and intraplant type crosses involving both parents with high gca effects for yield and yield component characters in desirable direction. The robust parents RAB-5, RAH-223 and 4350504 were the most potential combiners based on per cent gca method in diallel study. Based on simple pooled and per cent gca method the lines 4350407,BCCH-3C3 and RAH-223 are recognized as the most potential parents and RACH-221 was recognized as the most potential tester in line x tester analysis. Among the crosses studied, robust x compact crosses were more potential, this is because there is blending of desirable traits coming from robust and compact plant types, such as utilization of additional three dimensional space as seen in robust and efficient packing of bolls as observed in compact plant types. Path analysis has revealed that lint yield followed by number of bolls per plant had highest positive direct effect on seed cotton yield.
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    ThesisItemOpen Access
    EFFECT OF BLOOMING DATES ON YIELD, YIELD CONTRIBUTING AND FIBRE QUALITY PARAMETERS AND INTROGRESSION OF FIBRE QUALITIES IN Gossypium spp.
    (University of Agricultural Sciences, Dharwad, 2003) MANOHAR, K K; KATAGERI, I S
    Cotton is a long duration crop. The flowering starts generally after 45 to 50 days and continued upto 150 days after sowing. It indicates nearly 100 or more than 100 days are involved only for kapas production. So, the boll development period would subject to different macro or micro environmental changes. They will thus affect the yield of seed cotton and its quality. Therefore, the present study was aimed at screening cotton varieties which are less influenced by environmental factors during flowering to harvesting by way of studying effect of blooming dates on kapas yield and quality of fibre properties. Other study carried out was with an objective of identifying strong and long fibred G. hirsutum cotton. The present study was undertaken during kharif 2001-02 at Agricultural Research Station, Oharwad. The effect of blooming dates on yield and fibre properties was carried out in advance generation genetic lines belonging to G. hirsutum, G. arboreum and G. herbaceum. Selection of recombinant lines for superior fibre properties was carried out in F4 generation interspecific cross, DCH-32. Among G. hirsutum lines, CPD-431 and CPD-755 showed insignificant difference between means of first four weeks and second four weeks for number of flowers per plant, boll weight, ginning outturn and lint index to indicate their stability or their lesser sensitiveness to micro-climatical changes. Similarly, PA-304 and GAM-69 among the G. arboreum lines and DDhc-13 in G. herbaceum lines have respectively showed insignificant difference between the means of first four weeks and second four weeks for number of flowers, boll weight, ginning outturn, seed index and number of flowers, boll weight, seed cotton yield and lint index to indicate their lesser sensitiveness to micro-climatical changes. Recombinant G. hirsutum lines with superior fibre properties from G. barbadense were obtained. They recorded 30 - 33 mm fibre length with fibre strength of 22 - 24 g per tex.
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    ThesisItemOpen Access
    STABILITY ANALYSIS OF PUBLIC AAID PRIVATE BRED HYBRIDS OF KHARIF SORGHUM [Sorghum bicolor (L.) Moench]
    (University of Agricultural Sciences, Dharwad, 2003) MRUTHYUNJAYA, N; NAYAKAR, N Y
    ABSTRACT NOT AVAILABLE