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Subject: Agricultural Meteorology × Author: Rajashkar M. Uppin × End date: 2017 × Type: Thesis ×
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    ThesisItemOpen Access
    Isolation and Charcterization of butanol Producing Clostridiumisolates from Diverse Sources
    (UAS, Dharwad, 2013) Rajashkar M. Uppin; Geeta G. Shirnalli
    There is an incessant fluctuation in oil prices besides increasing stress from environmental pollution. The microbial production of biofuel from renewable sources is gaining interest. Biobutanol has advantages over traditional fuel ethanol in terms of energy density and hygroscopicity. Therefore, the present study was conducted to isolate and characterize butanol producing Clostridium sp from diverse sources. In the present study, 42 samples including soil, spoilt fruits etc. were collected from various natural sources from different locations of zone 8 of North Karnataka. A total of 130 biobutanol producing Clostridium isolates were isolated following standard Hungates roll tube technique. All the isolates that were Gram positive and forming endospores were considered to be Clostridium sp. Among these 47 isolates showed positive for indole production and gelatin liquefaction which are the main characters of Butanol producing Clostridium sp. Based on the rifampicin sensitivity, curd formation and riboflavin production the isolates were tentatively identified as, C. acetobutylicum, C.NCP 262, C. saccharoper butylacetonicum and C. beijerinckii. Fifteen isolates were selected based on their sources for detection of organic acids viz., acetic, propionic and butyric acid by high performance liquid chromatography. The highest production of acetic acid 37.11 mg ml-1 was detected in CL-26(3) and the highest butyric acid of 3.49 mg ml-1 was detected in CL-12(2) and the highest production of propionic acid was found in CL-40(4) was 30.69 mg ml-1, The strain ATCC 824 produced 30.80 mg ml-1 of acetic acid, 6.85 mg ml-1 of butyric acid and 0.61 mg ml-1 of propionic acid respectively. The wide variations in the production of organic acids indicated genetic variability. Majority of the isolates belong to C. acetobutylicum, which was habituated in diverse sources. Thus, efficient strains can be screened for butanol production from biomass.