Thumbnail Image

Thesis

Browse

2000 - 200912010 - 201740
Reset filters
Subject: Agricultural Biotechnology × Start date: 1961 × Thesis Type: M.Sc ×
Reset filters

Search Results

Now showing 1 - 9 of 41
  • Thumbnail Image
    ThesisItemOpen Access
    Identification of Single Chain Fragment Variable Clones Expressing Monoclonal Antibody Against Potato Leaf Roll Virus Coat Protein
    (University of Agricultural Science, Dharwad, 2017-09) Shivaranjini C.; Moger, Narayan
  • Thumbnail Image
    ThesisItemOpen Access
  • Thumbnail Image
    ThesisItemOpen Access
    Genetic Investigations on Heat Adaptive Traits in Bread Wheat by Using Molecular Markers
    (University of Agricultural Science, Dharwad, 2016-11) Jaya; Biradar, Suma S.
  • Thumbnail Image
    ThesisItemOpen Access
    Evaluation and Characterization of Genetically Modified Cotton G. herbaceum Var. Jayadhar for Helicoverpa armigera Resistance
    (University of Agricultural Science, Dharwad, 2016-11) Mahawar, Sonam; Katageri, I.S.
  • Thumbnail Image
    ThesisItemOpen Access
    Studies on the Molecular Basis of Resistance Against Asian Soybean Rust in Advanced Lines
    (University of Agricultural Science, Dharwad, 2017-01) Vats, Gautam; Jahagirdar, Shamarao
  • Thumbnail Image
    ThesisItemOpen Access
    Molecular Interaction of Actinobacteria and Arbuscular Mycorrhiza with Fusarium oxysporum f. sp. lycopersici in Tomato (Solanum lycopersicum)
    (University of Agricultural Science, Dharwad, 2017-04) Gadag, Anusha Suresh; Krishnaraj, P.U.
    Fusarium oxysporum f.sp. lycopersici (FOL) is a soil borne plant pathogen causing Fusarium wilt an important and destructive fungal plant diseases worldwide. The pathogenic strain of FOL was isolated from the infested plant samples and the identification of the isolate were done by cultural characteristics, pathogenicity test and PCR amplification by using specific primers and sequencing of ITS region in the 18S rDNA gene. Initially, 80 isolates of the actinobacteria were screened against FOL. Out of these, six isolates showed significant inhibition. Further, three of these were selected for the in vivo evaluation under greenhouse conditions. Arbuscular mycorrhiza (AM) culture was collected from the Department of Agricultural Microbiology, UAS, Dharwad. Under challenge inoculation with FOL, the actinobacterial isolate AUDT 626 alone and in combination with AM culture showed significant control of the wilt disease compared to others. Growth parameters such as shoot length, root length and total dry weight were observed to be far superior in this treatment. Finally, the transcriptional analysis of the tomato plants was carried out using suppression subtractive hybridization (SSH). SSH products were sequenced on Ion torrent NGS platform and data was processed and function was annotated to identify differentially expressed transcripts. The dominant transcripts which were expressed were pathogenesis related genes expressed during the interaction in the leaves in responce to inoculation of AUDT 626 alone and in with AM fungi in the presence of the pathogen. Results revealed 61 and 240 well-annotated genes expressed due to inoculation of AUDT 626 alone and with AM treated respectively. Upregulated transcripts correspond to the general stress, pathogen and abiotic stress and other function. Few expressed transcripts were of unknown function. Actinobacterial strain, AUDT 626 and AM fungus controlled the wilt disease in tomato probably by mediating induced systematic resistance and contributed to plant growth and development.
  • Thumbnail Image
    ThesisItemOpen Access
    Marker Aided Introgression of Blast Resistance Genes Pi1, Pi2 and Pi54 Into Intan Rice Variety
    (University of Agricultural Science, Dharwad, 2016-12) Debnath, Prasenjit; Prashanthi S.K.
    Rice blast caused by Magnaporthe oryzae is the most devastating fungal disease that causes approximately 80 % yield loss. Use of resistant cultivars is the most effective and economical way to control rice blast disease. “Intan” is a medium slender indica variety, popular with farmers and consumers in Karnataka but highly susceptible to blast disease. BPT5204 NIL-28, 18, 30 introgressed with Pi1, Pi2 and Pi54 which was developed in the Department of Biotechnology, UAS, Dharwad and Tetep, having Pi1 and Pi54 were used as donor parents in the crossing programme to develop F1’s, BC1F1’s and BC2F1’s. Marker assisted backcross breeding approach was adopted to introgress broad spectrum blast resistance genes Pi1, Pi2 and Pi54 independently and pyramided into Intan in 2015 & 2016. Molecular markers genic/linked, flanking and unlinked to target genes were used as foreground, recombinant and background selection markers respectively. Genic marker RM224 for Pi1 gene, tightly linked marker AP5659-5 (0.10 cM) for Pi2 gene, RM206 (0.6 cM) for Pi54 gene showed polymorphism among the parents. These polymorphic markers were employed to confirm target genes in hybrids and backcross population. F1 plants generated from ‘Intan x Tetep’ cross was confirmed for the presence of Pi1 + Pi54 genes and confirmed plants were backcrossed. Further, these F1 plants were challenge inoculated with Magnaporthe oryzae isolates and F1’s showed resistant reaction, confirming the hybridity. Foreground selection was exercised and heterozygous plants with Pi1, Pi54, Pi1 + Pi54 pyramids were identified in BC1F1 generation. Sixty three genome wide markers were subjected for polymorphism and polymorphic markers were employed for background selection. From ‘Intan x BPT5204 NIL-18’ cross, heterozygous plants for Pi2 gene were confirmed in BC2F1 generation and subjected for background selection, in which recurrent parent genome recovery ranged from 57.40 % to 81.48 %.
  • Thumbnail Image
    ThesisItemOpen Access
    Generation of Mapping Population and Identification of Molecular Markers Associated with Powdery Mildew Resistance in Mungbean
    (University of Agricultural Science, Dharwad, 2017-07) Pooja S.K.; Bhat, Sumangala
    The present study was conducted to evaluate mungbean accessions for powdery mildew resistance, identify polymorphic markers between powdery mildew resistant and susceptible parents and validate molecular markers previously reported to be associated with powdery mildew resistance, at the University of Agriculture Sciences, Dharwad. Phenotypic screening of 130 mungbean accessions (procured from IIPR, Kanpur and AICRP on MULLaRP, UAS, Dharwad) including susceptible check (DGGV2) under natural epiphytotic condition was carried out at the Main Agriculture Research Station during kharif and rabi, 2016 using augmented design. During kharif, four genotypes (NUL- 7, GPM- 19, V. umbellata and V. trilobata) showed immune response, three genotypes viz., COGG- 913, VAIBHAV and TARM1 showed moderate resistance, six genotypes were rated as susceptible and the remaining 119 genotypes were rated as highly susceptible. During rabi, only three genotypes viz., GPM-19, V. umbellata and V. trilobata showed immune response, four genotypes (NUL-7, COGG-913, TARM1 and Vaibhav) were moderately resistant, nine genotypes showed susceptible reaction and the remaining 114 genotypes were rated as highly susceptible to the disease. High heritability and genetic advance over mean was observed for percent disease index (PDI) among 130 mungbean accessions. The molecular experiments were conducted in the Department of Biotechnology. Out of 64 SSR markers used for polymorphism survey between powdery mildew susceptible (DGGV2) and previously identified resistant (TARM1) parents, only four markers (CEDG121, CEDG245, MB-SSR238 and GMES5773) showed polymorphism. F1s were confirmed using polymorphic marker (MB-SSR238) and confirmed F1s were selfed to obtain F2. F2 phenotypic ratio showed good fit for 3R:1S indicating resistance to powdery mildew in TARM1 line is controlled by single dominant gene. In the present study, marker MB-SSR238 (found to be linked to qPMR2) showed significant association with powdery mildew resistance through single marker analysis, explaining the phenotypic variance of 11.64 %.
  • Thumbnail Image
    ThesisItemOpen Access
    Molecular Interaction of Arabidopsis-Rhizobacteria for Induced Systemic Tolerance Under Water Deficit Condition
    (University of Agricultural Science, Dharwad, 2017-07) Basavaarya, B.R.; Krishnaraj, P.U.
    Root colonization by actinobacteria induces systemic drought tolerance in Arabidopsis thaliana. Actinobacterial cultures were taken from culture repository of Department of Biotechnology, UAS, Dharwad on February, 2015. Experiment was conducted in vitro at the Department of Biotechnology. Collected isolates were screened under water deficit condition using Poly Ethylene Glycol 6000 (PEG) and tested for the production of Indole Acetic Acid (IAA), a plant growth promoting harmone. Three actinobacterial cultures were tested for their rhizospheric interaction with Arabidopsis thaliana plants under water deficit conditions. The isolate AUDT 651 promoted good root (10.68 cm) and shoot (11.38 cm) growth better than AUDT 545 and AUDT 605. Illumina RNA seq was performed to identify differentially expressed genes from plants inoculated with or without actinobacteria under drought stressed conditions and normal growth conditions. Only genes having a log2-fold change ≥ 2.0 or ≤ -2.0 and an adjusted p-value < 0.05, were included in present analysis of differential gene expression. While genes involved in metabolism, transport and abiotic stress tolerance were up regulated in actinobacterial inoculated conditions, stress responsive genes like scavenging enzymes (catalase, superoxide dismutase, peroxidase), LEA protein and ethylene-responsive transcription factor were down regulated. Transcripts of photosynthetic genes (cytochrome b6-f complex subunit, photosystem II reaction center protein), and transporter genes (detoxification protein, chloroplastic) were uniquely expressed in actinobacterial colonized plants. These data show how gene expression in plants in water deficit condition can be influenced by microbial colonization leading to plant protection, induced by the presence of actinobacteria in the rhizosphere.