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2017 - 201818
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Subject: Agricultural Biotechnology × End date: 2018 × Start date: 2017 ×
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Now showing 1 - 9 of 18
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    ThesisItemOpen Access
    Identification of Single Chain Fragment Variable Clones Expressing Monoclonal Antibody Against Potato Leaf Roll Virus Coat Protein
    (University of Agricultural Science, Dharwad, 2017-09) Shivaranjini C.; Moger, Narayan
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    Molecular Basis of Moisture Stress Tolerance in Cotton (G. hirsutum)
    (University of Agricultural Sciences, Dharwad, 2018-02) Thakur, Pranita Prabhakar; Biradar, D.P.
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    ThesisItemOpen Access
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    Molecular Interaction Between Rhizobacteria and Root Pathogens in Groundnut (Arachis hypogea L.) and Brinjal (Solanum melongena L.)
    (University of Agricultural Science, Dharwad, 2017-11) Roopa K.P.; Krishnaraj, P.U.
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    ThesisItemOpen Access
    Studies on the Molecular Basis of Resistance Against Asian Soybean Rust in Advanced Lines
    (University of Agricultural Science, Dharwad, 2017-01) Vats, Gautam; Jahagirdar, Shamarao
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    ThesisItemOpen Access
    Molecular Interaction of Actinobacteria and Arbuscular Mycorrhiza with Fusarium oxysporum f. sp. lycopersici in Tomato (Solanum lycopersicum)
    (University of Agricultural Science, Dharwad, 2017-04) Gadag, Anusha Suresh; Krishnaraj, P.U.
    Fusarium oxysporum f.sp. lycopersici (FOL) is a soil borne plant pathogen causing Fusarium wilt an important and destructive fungal plant diseases worldwide. The pathogenic strain of FOL was isolated from the infested plant samples and the identification of the isolate were done by cultural characteristics, pathogenicity test and PCR amplification by using specific primers and sequencing of ITS region in the 18S rDNA gene. Initially, 80 isolates of the actinobacteria were screened against FOL. Out of these, six isolates showed significant inhibition. Further, three of these were selected for the in vivo evaluation under greenhouse conditions. Arbuscular mycorrhiza (AM) culture was collected from the Department of Agricultural Microbiology, UAS, Dharwad. Under challenge inoculation with FOL, the actinobacterial isolate AUDT 626 alone and in combination with AM culture showed significant control of the wilt disease compared to others. Growth parameters such as shoot length, root length and total dry weight were observed to be far superior in this treatment. Finally, the transcriptional analysis of the tomato plants was carried out using suppression subtractive hybridization (SSH). SSH products were sequenced on Ion torrent NGS platform and data was processed and function was annotated to identify differentially expressed transcripts. The dominant transcripts which were expressed were pathogenesis related genes expressed during the interaction in the leaves in responce to inoculation of AUDT 626 alone and in with AM fungi in the presence of the pathogen. Results revealed 61 and 240 well-annotated genes expressed due to inoculation of AUDT 626 alone and with AM treated respectively. Upregulated transcripts correspond to the general stress, pathogen and abiotic stress and other function. Few expressed transcripts were of unknown function. Actinobacterial strain, AUDT 626 and AM fungus controlled the wilt disease in tomato probably by mediating induced systematic resistance and contributed to plant growth and development.
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    ThesisItemOpen Access
    Generation of Mapping Population and Identification of Molecular Markers Associated with Powdery Mildew Resistance in Mungbean
    (University of Agricultural Science, Dharwad, 2017-07) Pooja S.K.; Bhat, Sumangala
    The present study was conducted to evaluate mungbean accessions for powdery mildew resistance, identify polymorphic markers between powdery mildew resistant and susceptible parents and validate molecular markers previously reported to be associated with powdery mildew resistance, at the University of Agriculture Sciences, Dharwad. Phenotypic screening of 130 mungbean accessions (procured from IIPR, Kanpur and AICRP on MULLaRP, UAS, Dharwad) including susceptible check (DGGV2) under natural epiphytotic condition was carried out at the Main Agriculture Research Station during kharif and rabi, 2016 using augmented design. During kharif, four genotypes (NUL- 7, GPM- 19, V. umbellata and V. trilobata) showed immune response, three genotypes viz., COGG- 913, VAIBHAV and TARM1 showed moderate resistance, six genotypes were rated as susceptible and the remaining 119 genotypes were rated as highly susceptible. During rabi, only three genotypes viz., GPM-19, V. umbellata and V. trilobata showed immune response, four genotypes (NUL-7, COGG-913, TARM1 and Vaibhav) were moderately resistant, nine genotypes showed susceptible reaction and the remaining 114 genotypes were rated as highly susceptible to the disease. High heritability and genetic advance over mean was observed for percent disease index (PDI) among 130 mungbean accessions. The molecular experiments were conducted in the Department of Biotechnology. Out of 64 SSR markers used for polymorphism survey between powdery mildew susceptible (DGGV2) and previously identified resistant (TARM1) parents, only four markers (CEDG121, CEDG245, MB-SSR238 and GMES5773) showed polymorphism. F1s were confirmed using polymorphic marker (MB-SSR238) and confirmed F1s were selfed to obtain F2. F2 phenotypic ratio showed good fit for 3R:1S indicating resistance to powdery mildew in TARM1 line is controlled by single dominant gene. In the present study, marker MB-SSR238 (found to be linked to qPMR2) showed significant association with powdery mildew resistance through single marker analysis, explaining the phenotypic variance of 11.64 %.