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Subject: Agricultural Biotechnology × Author: Hegde, Radhika D. × End date: 2016 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Physio-Biochemical and Molecular Characterization of Gm Cotton for Moisture Stress Resistance
    (University of Agricultural Science, Dharwad, 2016-08) Hegde, Radhika D.; Vamadevaiah, H.M.
    Cotton (Gossypium sps.) is important fibre crop known as white gold. The life of nation directly or indirectly depend on cotton, as crop, cloth, culture on daily basis. Quality and quantity of cotton yield influenced by the moisture stress. Drought resistance is accompanied by number of traits and they are regulated by many genes. Hence, transcription factor technology was used to develop drought resistant transgenic cotton. With AtDREB1A gene transgenic cotton (Gossypium hirsutum L.) was developed and regenerated through somatic embryogenesis at the Agriculture Research Station (ARS), Dharwad by previous workers. The free independent events of T2 generation transgenic plants were evaluated for moisture stress in the present study. Experimental sets were carried out in the transgenic green house at ARS, Dharwad. Seeds of independent transgenic events were sown in the polythene bags separately. Up to 45 DAS, two blocks were irrigated regularly, then irrigation was withheld in one of the block. 75 DAS plants started experiencing moisture stress. During this period DREB gene fold expression was relatively higher in the transgenic events compared to non-transgenic plant. Also, its target gene LEA protein gene exhibited relatively higher level of fold expression in transgenic events. The physiological and biochemical study also revealed that higher accumulation of the osmo-protectants like, proline, reducing sugar in the transgenic events compared to the negative control. Even at severe moisture stress, transgenic events maintained significantly higher level of relative water content, lower leaf water potential which are essential for the normal cell activity. Also maintained significantly higher stomatal conductance, photosynthetic rate, canopy temperature compared to negative control. By this study characterized GM-23, GM-14, GM-28, GM-11 and GM-30 were superior five events among selected events